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over time ligation - (Nov/28/2005 )

What happens if I make a ligation more than 16 hours?



At which temperature?


Blunt or sticky ends?


At 16C I generally do for 24 hrs(during weekends ).. works better for me...


It doesn't matter over 16h and may has some good effect on your ligation efficiency.


I made the experience, that people who ligate over night just want to go home biggrin.gif