over time ligation - (Nov/28/2005 )
What happens if I make a ligation more than 16 hours?
At which temperature?
Blunt or sticky ends?
At 16C I generally do for 24 hrs(during weekends ).. works better for me...
It doesn't matter over 16h and may has some good effect on your ligation efficiency.
I made the experience, that people who ligate over night just want to go home