Protocol:GFP reporter assay - (Aug/10/2006 )
Can anyone provide me with the detail protocol of GFP reporter assay.
Simple, examine your cells under a fluorescence microscope using FITC (green) filter.
Thanks, one more stupid question. Can I do it in a 96- well plate and What is the wavelength?
ur microscope will have a filter for it.
excitation peak at 395 nm & emission peak is at 509 nm-GFP
excitation peak is 490 nm with the peak emission kept at 509 nm - EGFP
our scope has 480/535 set of filters.