How to get GFP producing bacteria? - help? (Oct/22/2007 )
Hi!
I wonder if anyone knows where I can obtain GFP producing bacteria? I only want to study the growth, not to look at protein expression or anything.
To 'make' own GFP strains is unfortunetly not within the capacity of my lab 
I don't quite get it...
if you don't want to produce the GFP, then why do you need it to be GFP+???
Anyway, you can obtain the GFP plasmid and transform it into any strain of your choice... Someone in the labs near yours may have it.
Carolina Biological sells kits to high schools for doing transformations. They may even have control strains that are already transformed, but you should have no trouble with one of their kits. They are cheap, also.
if you don't want to produce the GFP, then why do you need it to be GFP+???
Anyway, you can obtain the GFP plasmid and transform it into any strain of your choice... Someone in the labs near yours may have it.
Perhhaps I was a little bit unclear
I want to produce GFP, but not as a reporter gene or anything, just for flourescence.
Acctually, I'm working in a lab that deals with celulosic fibre and wood chemistry, so no plasmid in sight. But thanks for the suggestion!
ooh they were cheap! and seemed to be simple, too. but unfortunately I live in Sweden, that don't sell any products here =(
Best way is to ask around. Every genetics lab has a regular E. coli expressing GFP constitutively, from a chromosomal copy or a multicopy plasmid.
But how do you want to study growth? Using a spectrofluorimeter? A confocal microscope?
Growth is usually basically studied using plate counts on selective media...
Regards,
T.
if you don't want to produce the GFP, then why do you need it to be GFP+???
Anyway, you can obtain the GFP plasmid and transform it into any strain of your choice... Someone in the labs near yours may have it.
Perhhaps I was a little bit unclear
I want to produce GFP, but not as a reporter gene or anything, just for flourescence.
Acctually, I'm working in a lab that deals with celulosic fibre and wood chemistry, so no plasmid in sight. But thanks for the suggestion!