BrdU incorporation and GFP staining - (Sep/02/2006 )
Can anyone suggest methods of how to do IF for both BrdU incorporation and GFP staining in mammalian cells? The normal method of BrdU IF requires heat or acid to denature the DNA with seems to be destroying the GFP epitope. I know Roche offers a kit that has anti-Brdu antibody with nucleases (no acid or heat needed) but the kit comes with enough BrdU labelling to label a city but only enough antibody for a few slides and it can't be saved/reused. They refuse to sell the antibody seperately and the kit is VERY expensive.
Try the Ab from Amersham: it comes with DNase as well and they give you a lot