invisible GFP - (Dec/03/2004 )
I just received cells tranfected with receptor-GFP.
I can't see GFP by direct microscopy. The person who send me cells see a great GFP signal...
But by Western and IF I can say that GFP is in my cells ( and also the receptor ).
She send cells twice but it's always the same problem.
Anyone already have the same probl ?
Thanks for reply
That may sound a little too simple, but do you watch your cells in the dark?? GFP needs to be excited so it can emit some green light.
Excitation wavelength : 488nm
Emission wavelength : 509nm
The light from a room is enough, generally.
of course i protect the cells from light during fixation etc......
and it's not a problem of wavelenght, I work often on confocal.....
thx 4 ur reply