Has anybody added artificial introns into GFP coding sequence. - (Aug/13/2007 )
Just like the topic! But I know really little about the mechanisms of intron splicing. So can anybody please tell me how could I make sure the intron I inserted can be spliced (in microinjected zebrafish embryos).
Any suggestions will be greatly appreciated!
Thank you very much.
Wei.
-xiawei-
QUOTE (xiawei @ Aug 13 2007, 08:51 AM)
Just like the topic! But I know really little about the mechanisms of intron splicing. So can anybody please tell me how could I make sure the intron I inserted can be spliced (in microinjected zebrafish embryos).
Any suggestions will be greatly appreciated!
Thank you very much.
Wei.
Any suggestions will be greatly appreciated!
Thank you very much.
Wei.
People have made vectors with intron sequence following the cDNA sequence. It helps expression by a couple of folds.
If the intron is before the cDNA sequence, I think you need to check for splice acceptor and splice donor sequences to verify if intron will be spliced out properly.
-scolix-