GFP - (May/20/2003 )
I would like to know if there will be any fluorscent if I transfact empty GFP vector into the cell? It's that a must to do some negative control during the GFP transfection experiment like transfect empty vector or no transfection? Or any other negative control should be included?
thanks very much
Is there a promotor in front of the GFP coding sequence in the vector? Otherwise GFP will be be expressed.