GFP transfection using lipofectin - (Nov/01/2001 )
I'd like to find out the conditions required to sucessfully transiently transfect HepG2 cells with a mammalian expression plasmid containing the GFP protein using lipofectin,:The amount of plasmid requiredThe amount of time required for the plasmid to be incubated on the cellsThe amount of lipofectin requiredThank you!
Normally, you would have to optimise the experiment for conditions like that. Try LifeTech's website www.lifetech.com for a massive pdf file containing everything you could know about transfections and optimisations for new cell lines.
Basically, you use a 24-well plate with different DNA concentrations down in each row and different lipofectin concentrations along each row...