question on colocalization of mitochondrion and a GFP fusion - (Dec/17/2007 )
Hi dear brothers and sisters:
I'm writing to consult you an urgent question about the colocalization of mitochondrion and a
transiently expressed GFP-nuclear encoded mitochondrial gene in onion
epidermal cells by particle bombardment.
For several times when I used the 'MitoTrcker Red CMXRos dye' purchased from invitrogene to stain the mitochondrion,
I failed to reach satisfied results：the red signals (should be emitted
from mitochondrion) seemed not overlapped with the green signals, which
should be resulted from the expression of the GFP fusion. Any one who likes to give advices please tell me your email adesses in your reply to this post, so that I can send you some pictures from my experiments. I'd
like you to help me to judge if the MitoTracker stained pattern is normal.
Hoping to hear from you as quickly as possible since I
have only limited time to improve this experiment! Thanks a lot in
advance.
Sincerely yours,
Paul
is the GFP signal in the cytoplasm or at the mitochondria?
I'm writing to consult you an urgent question about the colocalization of mitochondrion and a
transiently expressed GFP-nuclear encoded mitochondrial gene in onion
epidermal cells by particle bombardment.
For several times when I used the 'MitoTrcker Red CMXRos dye' purchased from invitrogene to stain the mitochondrion,
I failed to reach satisfied results：the red signals (should be emitted
from mitochondrion) seemed not overlapped with the green signals, which
should be resulted from the expression of the GFP fusion. Any one who likes to give advices please tell me your email adesses in your reply to this post, so that I can send you some pictures from my experiments. I'd
like you to help me to judge if the MitoTracker stained pattern is normal.
Hoping to hear from you as quickly as possible since I
have only limited time to improve this experiment! Thanks a lot in
advance.
Sincerely yours,
Paul
try to show me some images of the gfp-fusion and mitotracker