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Transformation Related Discussions
  1. prepare competent cells from existing competent cells - (reply: 2)
  2. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
  3. propagating commercial chemically competent cells - (reply: 2)
  4. Failed Gibson transformation - (reply: 7)
  5. Storing Ligated/Transformation Material before Transfection - (reply: 1)
  6. Overcoming methylation of bacterial DNA which inhibits transformation and genome - (reply: 6)
  7. Loss of protein expression by transformed bacteria - (reply: 4)
  8. Weird results with bacterial transformation - (reply: 9)
  9. How much plasmid for transformation? - (reply: 8)
  10. Alternative competent cells for QuikChange kit - (reply: 2)
  11. Transformation E.Coli help - (reply: 2)
  12. Transformation in Staphylococcus epidermidis - (reply: 1)
  13. transformation - (reply: 2)
  14. Difficult cloning/ligation/transformation blunt and sticky - (reply: 1)
  15. TOP10F´ Chemically Competent E. coli - (reply: 1)
  16. Competent cells with Hanahan's method - (reply: 2)
  17. Using more transformed bacteria for midiprep - (reply: 2)
  18. No colonies for transformation - (reply: 8)
  19. Help needed for Ligation/Transformation - (reply: 1)
  20. DNA cleanup after linearization, before transformation? - (reply: 3)
  21. Checking for efficieny competent cells - (reply: 1)
  22. Problem in plasmid digestion after transformation - (reply: 4)
  23. Transformed Agro colonies growing very slowly - (reply: 1)
  24. Transformation keeps failing - (reply: 2)
  25. Yeast high-efficiency transformation - (reply: 3)
  26. freezing competent cells without dry ice or liquid N - (reply: 3)
  27. bacterial transformation kit life-technologies - (reply: 1)
  28. transformation and protein purification calculation help; very basic - (reply: 5)
  29. Expansion of commercial competent bacterial cell lines? - (reply: 2)
  30. origami bacteria transformation - (reply: 5)
  31. Transformation of moss, Physcomitrella patens - (reply: 1)
  32. Problems with ligation and transformation - (reply: 2)
  33. Transformation of Restricted plasmid - (reply: 3)
  34. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
  35. ligation transformation - (reply: 4)
  36. Bacterial transformation problems - (reply: 1)
  37. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  38. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  39. DH5alpha transformation protocol - (reply: 1)
  40. SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
  41. colony PCR after transformation - (reply: 1)
  42. Low Transformation Efficiency with a Specific Gene - (reply: 4)
  43. Selection of E.coli competent cells - (reply: 2)
  44. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
  45. transformation problem - (reply: 8)
  46. double transformation problem - (reply: 3)
  47. competent cells - (reply: 1)
  48. Transformation colonies does not contain insert - (reply: 3)
  49. Screening for ligation and transformation result - (reply: 2)
  50. Maintainence of transformed cells - (reply: 5)
  51. How to select competent E. coli cells? - (reply: 7)
  52. SOC vs TB in transformations - (reply: 1)
  53. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  54. Ligation of large ammount of insert (no transformation) - (reply: 7)
  55. Transformation problem - (reply: 5)
  56. Transformation or Ligation problems - (reply: 4)
  57. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
  58. Freeze transformation mixture? - (reply: 2)
  59. Problems with growing out transformants in liquid LB - (reply: 3)
  60. How to transform adherent cells to suspension (CHO cells) - (reply: 2)
  61. my competent cell grow on any agar plate - (reply: 7)
  62. p14ARF and MDM2 in transformed cells - (reply: 1)
  63. Another cloning hell....no transformants - (reply: 1)
  64. Agrobacterium LBA4404 transformation - (reply: 1)
  65. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  66. surprising behavior of transformants needs immediate advice! - (reply: 8)
  67. Competent bacterial cells - (reply: 2)
  68. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 12)
  69. Transformed bacteria grows on plates but not in broth - (reply: 1)
  70. Transformation with a big plasmid - (reply: 4)
  71. SURE E. coli competent cells - (reply: 1)
  72. Subcloning - RE digest and ligation and transformation - (reply: 5)
  73. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  74. question about transformation - (reply: 1)
  75. Weird Library Transformation Results - (reply: 10)
  76. Clumpy competent cells - (reply: 3)
  77. competent cell formation - (reply: 1)
  78. Colonies from transformation plate are not growing...:( - (reply: 5)
  79. No growth following transformation - (reply: 1)
  80. Trouble Shooting for Transformation - (reply: 2)
  81. Sub-cloning and transformation - (reply: 17)
  82. Transformation of an inducible GFP gene into Aspergillus niger - (reply: 2)
  83. Problem with plasmids transformation - (reply: 6)
  84. Transformation Storage Buffer - too much MgSO4 - (reply: 2)
  85. An improve method for making Competent Cells without heat shock. How? - (reply: 2)
  86. Cloning Problem - Ligation or Transformation - (reply: 4)
  87. Using normal competent cells in electroporation method? - (reply: 1)
  88. recover old transformed DH5 - (reply: 1)
  89. tobacco transformation - (reply: 1)
  90. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  91. Methods of plasmid transformation? only heat shock? - (reply: 1)
  92. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
  93. Competent cells question: JM101 vs DH5alpha - (reply: 2)
  94. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  95. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  96. Plasmid digestion after transformation - (reply: 4)
  97. Making BL21 competent cells - (reply: 2)
  98. Transformation /Cloning Problem - (reply: 3)
  99. Transfection problem after transformation! - (reply: 5)
  100. bacterial transformation problem - (reply: 2)
  101. Transformation - (reply: 3)
  102. What does vortexing do in transformation? - (reply: 1)
  103. Host cells for transformation - (reply: 3)
  104. protocol for making e.coli competent cells under low temperature - (reply: 1)
  105. Condition for Transformation - heat shock, "A novice" - (reply: 2)
  106. Any competent or certain E Coli for mutagenesis transformation? - (reply: 1)
  107. Transformation bacteria heat chock and incubation? - (reply: 2)
  108. competent cells - (reply: 2)
  109. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  110. transformation fails, bacteria grows in control plate - (reply: 6)
  111. Transient Transformation of Nicotiana benthamiana leaves - (reply: 1)
  112. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  113. transformation of green algae - agrobac.mediated transformation... (reply: 3)
  114. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
  115. competent cells stock - (reply: 3)
  116. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
  117. ligation and transformation problem - (reply: 1)
  118. growing competent cells - (reply: 1)
  119. making more competent cells - (reply: 2)
  120. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
  121. Transformation & Minimal Plates - (reply: 5)
  122. Transformation issue - (reply: 1)
  123. yeast transformation - (reply: 1)
  124. Problems with ligation and transformation - (reply: 2)
  125. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  126. Reasons for failure of transformation - (reply: 11)
  127. Small Colonies After Transformation - (reply: 5)
  128. Ligation and transformation problem - (reply: 2)
  129. Agrobacterium EHA101 competent cells - (reply: 1)
  130. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
  131. Chemical competent E.coli always from single colony ? - (reply: 2)
  132. No colonies after transformation! - (reply: 2)
  133. questions about transformation - (reply: 4)
  134. Trouble with growth of transformed cells - (reply: 4)
  135. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
  136. Transformants Don't Grow - (reply: 4)
  137. TERT transformation of fibroblast cells - Immortilising fibroblast cells. Which companies available? (reply: 1)
  138. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
  139. DH5 Alpha Transformation - (reply: 2)
  140. TSS Transformation troubleshooting - no colonies on plate (reply: 4)
  141. Starting cells for making one's own competent cells - (reply: 7)
  142. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  143. double transformations - (reply: 1)
  144. Unusual band in transformants - (reply: 2)
  145. qPCR for transformed cells - (reply: 1)
  146. Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
  147. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
  148. H-ras transformed RK3E/tv-a cells - (reply: 1)
  149. Competent cell storage for long term use - (reply: 2)
  150. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
  151. Why does the amount of plamids transformed affect colony count? - (reply: 2)
  152. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  153. competent cells - OD600 reading (reply: 6)
  154. grow in broth but not on agar... - after transformation (reply: 2)
  155. verifying a positive transformant - (reply: 3)
  156. transformation efficiency - (reply: 7)
  157. transformed bacteria doesnt grow - (reply: 1)
  158. Transformation background problem - high background in negative control (reply: 9)
  159. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  160. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  161. What is the role of DMSO in yeast transformation - (reply: 1)
  162. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  163. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
  164. Transformation(?) issues - (reply: 3)
  165. ligation/transformation failed - (reply: 7)
  166. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
  167. BL21(DE3)LysS competent cells - (reply: 1)
  168. insert in the reverse orientation after transformation - (reply: 5)
  169. Biolistic (transformation efficiency) - (reply: 2)
  170. CaCl2 for making competent cells - (reply: 3)
  171. Extremely small bacterial colonies after transformation - (reply: 10)
  172. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
  173. help with transformation efficiency calculation! - (reply: 2)
  174. help with transformation efficiency calculation! - (reply: 1)
  175. dh5 alpha competent cells? - (reply: 2)
  176. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
  177. pre-prepararation of competent cells - (reply: 3)
  178. point mutation when transformed in BL21 - (reply: 2)
  179. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
  180. Screening colonies after transformation - (reply: 2)
  181. z competent cells - (reply: 2)
  182. macrophage transformation - (reply: 1)
  183. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
  184. have problems with electroporation transformation of PCP20 into BL21 de3 - (reply: 2)
  185. E coli transformation - (reply: 8)
  186. problem with yeast transformation - (reply: 2)
  187. Making competent cell from freeze-thawed stock - (reply: 2)
  188. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  189. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
  190. Bacteria transformation - (reply: 1)
  191. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
  192. Chosing the right competent cells - (reply: 1)
  193. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  194. ccdB survival E. coli transformation - (reply: 2)
  195. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
  196. cempetent e.coli and transformation - (reply: 2)
  197. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
  198. Yeast transformation - (reply: 1)
  199. FUNGI SILENCING - PROTOCOL TO TRANSFORM FUNGI CELLS (reply: 2)
  200. transformation medium - (reply: 2)
  201. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  202. same weird weird staph transformation results - any idea what is going on? (reply: 12)
  203. Picking strain for e coli competent cells - (reply: 6)
  204. is too much ligation reaction bad for transformation? - (reply: 4)
  205. please help!!! with transformation - (reply: 10)
  206. Failed Transformation of Tuner (DE3) cells - (reply: 1)
  207. Concentration of DNA for Transformation - (reply: 1)
  208. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  209. Non specific yeast transformations (or none at all) - (reply: 1)
  210. competent cell preparation? - (reply: 1)
  211. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  212. Competent Cells - (reply: 5)
  213. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  214. Mutation during transformation/maxiprep - (reply: 1)
  215. COMPETENT CELL PREPARATION - (reply: 6)
  216. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
  217. Transformation problem. Please help! - 1.7kb insert to 5.4kb vector; double digested w/ EcoRV & XhoI (reply: 17)
  218. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  219. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
  220. PCR problem from transformed TOPO TA vector - (reply: 3)
  221. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  222. calculating the mean change in a log transformed variable - is it possible? (reply: 1)
  223. transformation problem - (reply: 3)
  224. PCR screening of transformed bacterial colony - (reply: 6)
  225. Growing of TOP10 chemically competent cells - (reply: 4)
  226. Transform staph? - (reply: 2)
  227. YOU SHALL NOT PASS!! - bacteria will not transform..I think:/ (reply: 4)
  228. Help with transformation - (reply: 3)
  229. "false transformants" from B. subtilis transformation - how to get rid of them? (reply: 3)
  230. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
  231. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  232. help with ligation/transformation - (reply: 1)
  233. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
  234. help with transformation! thanks!! - (reply: 15)
  235. ligation - didn't got transformed colonies (reply: 1)
  236. Troubleshooting Digest, ligation, transformation - (reply: 7)
  237. Competent DH5 alpha - (reply: 4)
  238. what is co transformation scientificaly ? - what is co transformation scientificaly ? (reply: 1)
  239. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
  240. Chemically competent to electrocompetent DH5alpha - (reply: 1)
  241. To what extent competent cells be thawed before transformation? - (reply: 4)
  242. contamination in transformed bacteria - black filamentous fungi grows when culturing competent bacteria with m (reply: 8)
  243. Transformation question - (reply: 9)
  244. log transformed data - still not normal (reply: 2)
  245. making competent E coli DH5a cells - (reply: 6)