Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Transformation Related Discussions
  1. Loss of protein expression by transformed bacteria - (reply: 3)
  2. Weird results with bacterial transformation - (reply: 6)
  3. How much plasmid for transformation? - (reply: 8)
  4. Alternative competent cells for QuikChange kit - (reply: 2)
  5. Transformation E.Coli help - (reply: 2)
  6. Transformation in Staphylococcus epidermidis - (reply: 1)
  7. transformation - (reply: 2)
  8. Difficult cloning/ligation/transformation blunt and sticky - (reply: 1)
  9. TOP10F´ Chemically Competent E. coli - (reply: 1)
  10. Competent cells with Hanahan's method - (reply: 2)
  11. Using more transformed bacteria for midiprep - (reply: 2)
  12. No colonies for transformation - (reply: 8)
  13. Help needed for Ligation/Transformation - (reply: 1)
  14. DNA cleanup after linearization, before transformation? - (reply: 3)
  15. Checking for efficieny competent cells - (reply: 1)
  16. Problem in plasmid digestion after transformation - (reply: 4)
  17. Transformed Agro colonies growing very slowly - (reply: 1)
  18. Transformation keeps failing - (reply: 2)
  19. Yeast high-efficiency transformation - (reply: 3)
  20. freezing competent cells without dry ice or liquid N - (reply: 3)
  21. bacterial transformation kit life-technologies - (reply: 1)
  22. transformation and protein purification calculation help; very basic - (reply: 5)
  23. Expansion of commercial competent bacterial cell lines? - (reply: 2)
  24. origami bacteria transformation - (reply: 5)
  25. Transformation of moss, Physcomitrella patens - (reply: 1)
  26. Problems with ligation and transformation - (reply: 2)
  27. Transformation of Restricted plasmid - (reply: 3)
  28. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
  29. ligation transformation - (reply: 4)
  30. Bacterial transformation problems - (reply: 1)
  31. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  32. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  33. DH5alpha transformation protocol - (reply: 1)
  34. SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
  35. colony PCR after transformation - (reply: 1)
  36. Low Transformation Efficiency with a Specific Gene - (reply: 4)
  37. Selection of E.coli competent cells - (reply: 2)
  38. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
  39. transformation problem - (reply: 8)
  40. double transformation problem - (reply: 3)
  41. competent cells - (reply: 1)
  42. Transformation colonies does not contain insert - (reply: 3)
  43. Screening for ligation and transformation result - (reply: 2)
  44. Maintainence of transformed cells - (reply: 5)
  45. How to select competent E. coli cells? - (reply: 7)
  46. SOC vs TB in transformations - (reply: 1)
  47. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  48. Ligation of large ammount of insert (no transformation) - (reply: 7)
  49. Transformation problem - (reply: 5)
  50. Transformation or Ligation problems - (reply: 4)
  51. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
  52. Freeze transformation mixture? - (reply: 2)
  53. Problems with growing out transformants in liquid LB - (reply: 3)
  54. How to transform adherent cells to suspension (CHO cells) - (reply: 2)
  55. my competent cell grow on any agar plate - (reply: 7)
  56. p14ARF and MDM2 in transformed cells - (reply: 1)
  57. Another cloning hell....no transformants - (reply: 1)
  58. Agrobacterium LBA4404 transformation - (reply: 1)
  59. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  60. surprising behavior of transformants needs immediate advice! - (reply: 8)
  61. Competent bacterial cells - (reply: 2)
  62. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 12)
  63. Transformed bacteria grows on plates but not in broth - (reply: 1)
  64. Transformation with a big plasmid - (reply: 4)
  65. SURE E. coli competent cells - (reply: 1)
  66. Subcloning - RE digest and ligation and transformation - (reply: 5)
  67. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  68. question about transformation - (reply: 1)
  69. Weird Library Transformation Results - (reply: 10)
  70. Clumpy competent cells - (reply: 3)
  71. competent cell formation - (reply: 1)
  72. Colonies from transformation plate are not growing...:( - (reply: 5)
  73. No growth following transformation - (reply: 1)
  74. Trouble Shooting for Transformation - (reply: 2)
  75. Sub-cloning and transformation - (reply: 17)
  76. Transformation of an inducible GFP gene into Aspergillus niger - (reply: 2)
  77. Problem with plasmids transformation - (reply: 6)
  78. Transformation Storage Buffer - too much MgSO4 - (reply: 2)
  79. An improve method for making Competent Cells without heat shock. How? - (reply: 2)
  80. Cloning Problem - Ligation or Transformation - (reply: 4)
  81. Using normal competent cells in electroporation method? - (reply: 1)
  82. recover old transformed DH5 - (reply: 1)
  83. tobacco transformation - (reply: 1)
  84. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  85. Methods of plasmid transformation? only heat shock? - (reply: 1)
  86. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
  87. Competent cells question: JM101 vs DH5alpha - (reply: 2)
  88. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  89. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  90. Plasmid digestion after transformation - (reply: 4)
  91. Making BL21 competent cells - (reply: 2)
  92. Transformation /Cloning Problem - (reply: 3)
  93. Transfection problem after transformation! - (reply: 5)
  94. bacterial transformation problem - (reply: 2)
  95. Transformation - (reply: 3)
  96. What does vortexing do in transformation? - (reply: 1)
  97. Host cells for transformation - (reply: 3)
  98. protocol for making e.coli competent cells under low temperature - (reply: 1)
  99. Condition for Transformation - heat shock, "A novice" - (reply: 2)
  100. Any competent or certain E Coli for mutagenesis transformation? - (reply: 1)
  101. Transformation bacteria heat chock and incubation? - (reply: 2)
  102. competent cells - (reply: 2)
  103. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  104. transformation fails, bacteria grows in control plate - (reply: 6)
  105. Transient Transformation of Nicotiana benthamiana leaves - (reply: 1)
  106. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  107. transformation of green algae - agrobac.mediated transformation... (reply: 3)
  108. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
  109. competent cells stock - (reply: 3)
  110. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
  111. ligation and transformation problem - (reply: 1)
  112. growing competent cells - (reply: 1)
  113. making more competent cells - (reply: 2)
  114. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
  115. Transformation & Minimal Plates - (reply: 5)
  116. Transformation issue - (reply: 1)
  117. yeast transformation - (reply: 1)
  118. Problems with ligation and transformation - (reply: 2)
  119. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  120. Reasons for failure of transformation - (reply: 11)
  121. Small Colonies After Transformation - (reply: 5)
  122. Ligation and transformation problem - (reply: 2)
  123. Agrobacterium EHA101 competent cells - (reply: 1)
  124. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
  125. Chemical competent E.coli always from single colony ? - (reply: 2)
  126. No colonies after transformation! - (reply: 2)
  127. questions about transformation - (reply: 4)
  128. Trouble with growth of transformed cells - (reply: 4)
  129. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
  130. Transformants Don't Grow - (reply: 4)
  131. TERT transformation of fibroblast cells - Immortilising fibroblast cells. Which companies available? (reply: 1)
  132. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
  133. DH5 Alpha Transformation - (reply: 2)
  134. TSS Transformation troubleshooting - no colonies on plate (reply: 4)
  135. Starting cells for making one's own competent cells - (reply: 7)
  136. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  137. double transformations - (reply: 1)
  138. Unusual band in transformants - (reply: 2)
  139. qPCR for transformed cells - (reply: 1)
  140. Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
  141. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
  142. H-ras transformed RK3E/tv-a cells - (reply: 1)
  143. Competent cell storage for long term use - (reply: 2)
  144. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
  145. Why does the amount of plamids transformed affect colony count? - (reply: 2)
  146. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  147. competent cells - OD600 reading (reply: 6)
  148. grow in broth but not on agar... - after transformation (reply: 2)
  149. verifying a positive transformant - (reply: 3)
  150. transformation efficiency - (reply: 7)
  151. transformed bacteria doesnt grow - (reply: 1)
  152. Transformation background problem - high background in negative control (reply: 9)
  153. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  154. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  155. What is the role of DMSO in yeast transformation - (reply: 1)
  156. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  157. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
  158. Transformation(?) issues - (reply: 3)
  159. ligation/transformation failed - (reply: 7)
  160. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
  161. BL21(DE3)LysS competent cells - (reply: 1)
  162. insert in the reverse orientation after transformation - (reply: 5)
  163. Biolistic (transformation efficiency) - (reply: 2)
  164. CaCl2 for making competent cells - (reply: 3)
  165. Extremely small bacterial colonies after transformation - (reply: 10)
  166. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
  167. help with transformation efficiency calculation! - (reply: 2)
  168. help with transformation efficiency calculation! - (reply: 1)
  169. dh5 alpha competent cells? - (reply: 2)
  170. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
  171. pre-prepararation of competent cells - (reply: 3)
  172. point mutation when transformed in BL21 - (reply: 2)
  173. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
  174. Screening colonies after transformation - (reply: 2)
  175. z competent cells - (reply: 2)
  176. macrophage transformation - (reply: 1)
  177. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
  178. have problems with electroporation transformation of PCP20 into BL21 de3 - (reply: 2)
  179. E coli transformation - (reply: 8)
  180. problem with yeast transformation - (reply: 2)
  181. Making competent cell from freeze-thawed stock - (reply: 2)
  182. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  183. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
  184. Bacteria transformation - (reply: 1)
  185. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
  186. Chosing the right competent cells - (reply: 1)
  187. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  188. ccdB survival E. coli transformation - (reply: 2)
  189. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
  190. cempetent e.coli and transformation - (reply: 2)
  191. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
  192. Yeast transformation - (reply: 1)
  193. FUNGI SILENCING - PROTOCOL TO TRANSFORM FUNGI CELLS (reply: 2)
  194. transformation medium - (reply: 2)
  195. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  196. same weird weird staph transformation results - any idea what is going on? (reply: 12)
  197. Picking strain for e coli competent cells - (reply: 6)
  198. is too much ligation reaction bad for transformation? - (reply: 4)
  199. please help!!! with transformation - (reply: 10)
  200. Failed Transformation of Tuner (DE3) cells - (reply: 1)
  201. Concentration of DNA for Transformation - (reply: 1)
  202. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  203. Non specific yeast transformations (or none at all) - (reply: 1)
  204. competent cell preparation? - (reply: 1)
  205. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  206. Competent Cells - (reply: 5)
  207. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  208. Mutation during transformation/maxiprep - (reply: 1)
  209. COMPETENT CELL PREPARATION - (reply: 6)
  210. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
  211. Transformation problem. Please help! - 1.7kb insert to 5.4kb vector; double digested w/ EcoRV & XhoI (reply: 17)
  212. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  213. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
  214. PCR problem from transformed TOPO TA vector - (reply: 3)
  215. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  216. calculating the mean change in a log transformed variable - is it possible? (reply: 1)
  217. transformation problem - (reply: 3)
  218. PCR screening of transformed bacterial colony - (reply: 6)
  219. Growing of TOP10 chemically competent cells - (reply: 4)
  220. Transform staph? - (reply: 2)
  221. YOU SHALL NOT PASS!! - bacteria will not transform..I think:/ (reply: 4)
  222. Help with transformation - (reply: 3)
  223. "false transformants" from B. subtilis transformation - how to get rid of them? (reply: 3)
  224. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
  225. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  226. help with ligation/transformation - (reply: 1)
  227. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
  228. help with transformation! thanks!! - (reply: 15)
  229. ligation - didn't got transformed colonies (reply: 1)
  230. Troubleshooting Digest, ligation, transformation - (reply: 7)
  231. Competent DH5 alpha - (reply: 4)
  232. what is co transformation scientificaly ? - what is co transformation scientificaly ? (reply: 1)
  233. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
  234. Chemically competent to electrocompetent DH5alpha - (reply: 1)
  235. To what extent competent cells be thawed before transformation? - (reply: 4)
  236. contamination in transformed bacteria - black filamentous fungi grows when culturing competent bacteria with m (reply: 8)
  237. Transformation question - (reply: 9)
  238. log transformed data - still not normal (reply: 2)
  239. making competent E coli DH5a cells - (reply: 6)