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Transformation Related Discussions
  1. Failed Gibson transformation - (reply: 7)
  2. Storing Ligated/Transformation Material before Transfection - (reply: 1)
  3. Overcoming methylation of bacterial DNA which inhibits transformation and genome - (reply: 6)
  4. Loss of protein expression by transformed bacteria - (reply: 4)
  5. Weird results with bacterial transformation - (reply: 9)
  6. How much plasmid for transformation? - (reply: 8)
  7. Alternative competent cells for QuikChange kit - (reply: 2)
  8. Transformation E.Coli help - (reply: 2)
  9. Transformation in Staphylococcus epidermidis - (reply: 1)
  10. transformation - (reply: 2)
  11. Difficult cloning/ligation/transformation blunt and sticky - (reply: 1)
  12. TOP10F´ Chemically Competent E. coli - (reply: 1)
  13. Competent cells with Hanahan's method - (reply: 2)
  14. Using more transformed bacteria for midiprep - (reply: 2)
  15. No colonies for transformation - (reply: 8)
  16. Help needed for Ligation/Transformation - (reply: 1)
  17. DNA cleanup after linearization, before transformation? - (reply: 3)
  18. Checking for efficieny competent cells - (reply: 1)
  19. Problem in plasmid digestion after transformation - (reply: 4)
  20. Transformed Agro colonies growing very slowly - (reply: 1)
  21. Transformation keeps failing - (reply: 2)
  22. Yeast high-efficiency transformation - (reply: 3)
  23. freezing competent cells without dry ice or liquid N - (reply: 3)
  24. bacterial transformation kit life-technologies - (reply: 1)
  25. transformation and protein purification calculation help; very basic - (reply: 5)
  26. Expansion of commercial competent bacterial cell lines? - (reply: 2)
  27. origami bacteria transformation - (reply: 5)
  28. Transformation of moss, Physcomitrella patens - (reply: 1)
  29. Problems with ligation and transformation - (reply: 2)
  30. Transformation of Restricted plasmid - (reply: 3)
  31. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
  32. ligation transformation - (reply: 4)
  33. Bacterial transformation problems - (reply: 1)
  34. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  35. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  36. DH5alpha transformation protocol - (reply: 1)
  37. SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
  38. colony PCR after transformation - (reply: 1)
  39. Low Transformation Efficiency with a Specific Gene - (reply: 4)
  40. Selection of E.coli competent cells - (reply: 2)
  41. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
  42. transformation problem - (reply: 8)
  43. double transformation problem - (reply: 3)
  44. competent cells - (reply: 1)
  45. Transformation colonies does not contain insert - (reply: 3)
  46. Screening for ligation and transformation result - (reply: 2)
  47. Maintainence of transformed cells - (reply: 5)
  48. How to select competent E. coli cells? - (reply: 7)
  49. SOC vs TB in transformations - (reply: 1)
  50. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  51. Ligation of large ammount of insert (no transformation) - (reply: 7)
  52. Transformation problem - (reply: 5)
  53. Transformation or Ligation problems - (reply: 4)
  54. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
  55. Freeze transformation mixture? - (reply: 2)
  56. Problems with growing out transformants in liquid LB - (reply: 3)
  57. How to transform adherent cells to suspension (CHO cells) - (reply: 2)
  58. my competent cell grow on any agar plate - (reply: 7)
  59. p14ARF and MDM2 in transformed cells - (reply: 1)
  60. Another cloning hell....no transformants - (reply: 1)
  61. Agrobacterium LBA4404 transformation - (reply: 1)
  62. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  63. surprising behavior of transformants needs immediate advice! - (reply: 8)
  64. Competent bacterial cells - (reply: 2)
  65. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 12)
  66. Transformed bacteria grows on plates but not in broth - (reply: 1)
  67. Transformation with a big plasmid - (reply: 4)
  68. SURE E. coli competent cells - (reply: 1)
  69. Subcloning - RE digest and ligation and transformation - (reply: 5)
  70. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  71. question about transformation - (reply: 1)
  72. Weird Library Transformation Results - (reply: 10)
  73. Clumpy competent cells - (reply: 3)
  74. competent cell formation - (reply: 1)
  75. Colonies from transformation plate are not growing...:( - (reply: 5)
  76. No growth following transformation - (reply: 1)
  77. Trouble Shooting for Transformation - (reply: 2)
  78. Sub-cloning and transformation - (reply: 17)
  79. Transformation of an inducible GFP gene into Aspergillus niger - (reply: 2)
  80. Problem with plasmids transformation - (reply: 6)
  81. Transformation Storage Buffer - too much MgSO4 - (reply: 2)
  82. An improve method for making Competent Cells without heat shock. How? - (reply: 2)
  83. Cloning Problem - Ligation or Transformation - (reply: 4)
  84. Using normal competent cells in electroporation method? - (reply: 1)
  85. recover old transformed DH5 - (reply: 1)
  86. tobacco transformation - (reply: 1)
  87. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  88. Methods of plasmid transformation? only heat shock? - (reply: 1)
  89. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
  90. Competent cells question: JM101 vs DH5alpha - (reply: 2)
  91. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  92. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  93. Plasmid digestion after transformation - (reply: 4)
  94. Making BL21 competent cells - (reply: 2)
  95. Transformation /Cloning Problem - (reply: 3)
  96. Transfection problem after transformation! - (reply: 5)
  97. bacterial transformation problem - (reply: 2)
  98. Transformation - (reply: 3)
  99. What does vortexing do in transformation? - (reply: 1)
  100. Host cells for transformation - (reply: 3)
  101. protocol for making e.coli competent cells under low temperature - (reply: 1)
  102. Condition for Transformation - heat shock, "A novice" - (reply: 2)
  103. Any competent or certain E Coli for mutagenesis transformation? - (reply: 1)
  104. Transformation bacteria heat chock and incubation? - (reply: 2)
  105. competent cells - (reply: 2)
  106. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  107. transformation fails, bacteria grows in control plate - (reply: 6)
  108. Transient Transformation of Nicotiana benthamiana leaves - (reply: 1)
  109. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  110. transformation of green algae - agrobac.mediated transformation... (reply: 3)
  111. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
  112. competent cells stock - (reply: 3)
  113. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
  114. ligation and transformation problem - (reply: 1)
  115. growing competent cells - (reply: 1)
  116. making more competent cells - (reply: 2)
  117. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
  118. Transformation & Minimal Plates - (reply: 5)
  119. Transformation issue - (reply: 1)
  120. yeast transformation - (reply: 1)
  121. Problems with ligation and transformation - (reply: 2)
  122. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  123. Reasons for failure of transformation - (reply: 11)
  124. Small Colonies After Transformation - (reply: 5)
  125. Ligation and transformation problem - (reply: 2)
  126. Agrobacterium EHA101 competent cells - (reply: 1)
  127. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
  128. Chemical competent E.coli always from single colony ? - (reply: 2)
  129. No colonies after transformation! - (reply: 2)
  130. questions about transformation - (reply: 4)
  131. Trouble with growth of transformed cells - (reply: 4)
  132. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
  133. Transformants Don't Grow - (reply: 4)
  134. TERT transformation of fibroblast cells - Immortilising fibroblast cells. Which companies available? (reply: 1)
  135. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
  136. DH5 Alpha Transformation - (reply: 2)
  137. TSS Transformation troubleshooting - no colonies on plate (reply: 4)
  138. Starting cells for making one's own competent cells - (reply: 7)
  139. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  140. double transformations - (reply: 1)
  141. Unusual band in transformants - (reply: 2)
  142. qPCR for transformed cells - (reply: 1)
  143. Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
  144. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
  145. H-ras transformed RK3E/tv-a cells - (reply: 1)
  146. Competent cell storage for long term use - (reply: 2)
  147. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
  148. Why does the amount of plamids transformed affect colony count? - (reply: 2)
  149. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  150. competent cells - OD600 reading (reply: 6)
  151. grow in broth but not on agar... - after transformation (reply: 2)
  152. verifying a positive transformant - (reply: 3)
  153. transformation efficiency - (reply: 7)
  154. transformed bacteria doesnt grow - (reply: 1)
  155. Transformation background problem - high background in negative control (reply: 9)
  156. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  157. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  158. What is the role of DMSO in yeast transformation - (reply: 1)
  159. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  160. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
  161. Transformation(?) issues - (reply: 3)
  162. ligation/transformation failed - (reply: 7)
  163. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
  164. BL21(DE3)LysS competent cells - (reply: 1)
  165. insert in the reverse orientation after transformation - (reply: 5)
  166. Biolistic (transformation efficiency) - (reply: 2)
  167. CaCl2 for making competent cells - (reply: 3)
  168. Extremely small bacterial colonies after transformation - (reply: 10)
  169. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
  170. help with transformation efficiency calculation! - (reply: 2)
  171. help with transformation efficiency calculation! - (reply: 1)
  172. dh5 alpha competent cells? - (reply: 2)
  173. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
  174. pre-prepararation of competent cells - (reply: 3)
  175. point mutation when transformed in BL21 - (reply: 2)
  176. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
  177. Screening colonies after transformation - (reply: 2)
  178. z competent cells - (reply: 2)
  179. macrophage transformation - (reply: 1)
  180. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
  181. have problems with electroporation transformation of PCP20 into BL21 de3 - (reply: 2)
  182. E coli transformation - (reply: 8)
  183. problem with yeast transformation - (reply: 2)
  184. Making competent cell from freeze-thawed stock - (reply: 2)
  185. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  186. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
  187. Bacteria transformation - (reply: 1)
  188. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
  189. Chosing the right competent cells - (reply: 1)
  190. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  191. ccdB survival E. coli transformation - (reply: 2)
  192. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
  193. cempetent e.coli and transformation - (reply: 2)
  194. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
  195. Yeast transformation - (reply: 1)
  196. FUNGI SILENCING - PROTOCOL TO TRANSFORM FUNGI CELLS (reply: 2)
  197. transformation medium - (reply: 2)
  198. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  199. same weird weird staph transformation results - any idea what is going on? (reply: 12)
  200. Picking strain for e coli competent cells - (reply: 6)
  201. is too much ligation reaction bad for transformation? - (reply: 4)
  202. please help!!! with transformation - (reply: 10)
  203. Failed Transformation of Tuner (DE3) cells - (reply: 1)
  204. Concentration of DNA for Transformation - (reply: 1)
  205. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  206. Non specific yeast transformations (or none at all) - (reply: 1)
  207. competent cell preparation? - (reply: 1)
  208. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  209. Competent Cells - (reply: 5)
  210. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  211. Mutation during transformation/maxiprep - (reply: 1)
  212. COMPETENT CELL PREPARATION - (reply: 6)
  213. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
  214. Transformation problem. Please help! - 1.7kb insert to 5.4kb vector; double digested w/ EcoRV & XhoI (reply: 17)
  215. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  216. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
  217. PCR problem from transformed TOPO TA vector - (reply: 3)
  218. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  219. calculating the mean change in a log transformed variable - is it possible? (reply: 1)
  220. transformation problem - (reply: 3)
  221. PCR screening of transformed bacterial colony - (reply: 6)
  222. Growing of TOP10 chemically competent cells - (reply: 4)
  223. Transform staph? - (reply: 2)
  224. YOU SHALL NOT PASS!! - bacteria will not transform..I think:/ (reply: 4)
  225. Help with transformation - (reply: 3)
  226. "false transformants" from B. subtilis transformation - how to get rid of them? (reply: 3)
  227. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
  228. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  229. help with ligation/transformation - (reply: 1)
  230. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
  231. help with transformation! thanks!! - (reply: 15)
  232. ligation - didn't got transformed colonies (reply: 1)
  233. Troubleshooting Digest, ligation, transformation - (reply: 7)
  234. Competent DH5 alpha - (reply: 4)
  235. what is co transformation scientificaly ? - what is co transformation scientificaly ? (reply: 1)
  236. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
  237. Chemically competent to electrocompetent DH5alpha - (reply: 1)
  238. To what extent competent cells be thawed before transformation? - (reply: 4)
  239. contamination in transformed bacteria - black filamentous fungi grows when culturing competent bacteria with m (reply: 8)
  240. Transformation question - (reply: 9)
  241. log transformed data - still not normal (reply: 2)
  242. making competent E coli DH5a cells - (reply: 6)