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Transformation Related Discussions
  1. Competent cells with Hanahan's method - (reply: 2)
  2. Using more transformed bacteria for midiprep - (reply: 2)
  3. No colonies for transformation - (reply: 8)
  4. Help needed for Ligation/Transformation - (reply: 1)
  5. DNA cleanup after linearization, before transformation? - (reply: 3)
  6. Checking for efficieny competent cells - (reply: 1)
  7. Problem in plasmid digestion after transformation - (reply: 4)
  8. Transformed Agro colonies growing very slowly - (reply: 1)
  9. Transformation keeps failing - (reply: 2)
  10. Yeast high-efficiency transformation - (reply: 3)
  11. freezing competent cells without dry ice or liquid N - (reply: 3)
  12. bacterial transformation kit life-technologies - (reply: 1)
  13. transformation and protein purification calculation help; very basic - (reply: 5)
  14. Expansion of commercial competent bacterial cell lines? - (reply: 2)
  15. origami bacteria transformation - (reply: 5)
  16. Transformation of moss, Physcomitrella patens - (reply: 1)
  17. Problems with ligation and transformation - (reply: 2)
  18. Transformation of Restricted plasmid - (reply: 3)
  19. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
  20. ligation transformation - (reply: 4)
  21. Bacterial transformation problems - (reply: 1)
  22. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  23. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  24. DH5alpha transformation protocol - (reply: 1)
  25. SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
  26. colony PCR after transformation - (reply: 1)
  27. Low Transformation Efficiency with a Specific Gene - (reply: 4)
  28. Selection of E.coli competent cells - (reply: 2)
  29. Ligation/Transformation Mess-up, are they going to survive? - (reply: 5)
  30. transformation problem - (reply: 8)
  31. double transformation problem - (reply: 3)
  32. competent cells - (reply: 1)
  33. Transformation colonies does not contain insert - (reply: 3)
  34. Screening for ligation and transformation result - (reply: 2)
  35. Maintainence of transformed cells - (reply: 5)
  36. How to select competent E. coli cells? - (reply: 7)
  37. SOC vs TB in transformations - (reply: 1)
  38. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  39. Ligation of large ammount of insert (no transformation) - (reply: 7)
  40. Transformation problem - (reply: 5)
  41. Transformation or Ligation problems - (reply: 4)
  42. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
  43. Freeze transformation mixture? - (reply: 2)
  44. Problems with growing out transformants in liquid LB - (reply: 3)
  45. How to transform adherent cells to suspension (CHO cells) - (reply: 2)
  46. my competent cell grow on any agar plate - (reply: 7)
  47. p14ARF and MDM2 in transformed cells - (reply: 1)
  48. Another cloning hell....no transformants - (reply: 1)
  49. Agrobacterium LBA4404 transformation - (reply: 1)
  50. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  51. surprising behavior of transformants needs immediate advice! - (reply: 8)
  52. Competent bacterial cells - (reply: 2)
  53. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 11)
  54. Transformed bacteria grows on plates but not in broth - (reply: 1)
  55. Transformation with a big plasmid - (reply: 4)
  56. SURE E. coli competent cells - (reply: 1)
  57. Subcloning - RE digest and ligation and transformation - (reply: 5)
  58. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  59. question about transformation - (reply: 1)
  60. Weird Library Transformation Results - (reply: 10)
  61. Clumpy competent cells - (reply: 3)
  62. competent cell formation - (reply: 1)
  63. Colonies from transformation plate are not growing...:( - (reply: 5)
  64. No growth following transformation - (reply: 1)
  65. Trouble Shooting for Transformation - (reply: 2)
  66. Sub-cloning and transformation - (reply: 17)
  67. Transformation of an inducible GFP gene into Aspergillus niger - (reply: 2)
  68. Problem with plasmids transformation - (reply: 6)
  69. Transformation Storage Buffer - too much MgSO4 - (reply: 2)
  70. An improve method for making Competent Cells without heat shock. How? - (reply: 2)
  71. Cloning Problem - Ligation or Transformation - (reply: 4)
  72. Using normal competent cells in electroporation method? - (reply: 1)
  73. recover old transformed DH5 - (reply: 1)
  74. tobacco transformation - (reply: 1)
  75. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  76. Methods of plasmid transformation? only heat shock? - (reply: 1)
  77. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
  78. Competent cells question: JM101 vs DH5alpha - (reply: 2)
  79. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  80. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  81. Plasmid digestion after transformation - (reply: 4)
  82. Making BL21 competent cells - (reply: 2)
  83. Transformation /Cloning Problem - (reply: 3)
  84. Transfection problem after transformation! - (reply: 5)
  85. bacterial transformation problem - (reply: 2)
  86. Transformation - (reply: 3)
  87. What does vortexing do in transformation? - (reply: 1)
  88. Host cells for transformation - (reply: 3)
  89. protocol for making e.coli competent cells under low temperature - (reply: 1)
  90. Condition for Transformation - heat shock, "A novice" - (reply: 2)
  91. Any competent or certain E Coli for mutagenesis transformation? - (reply: 1)
  92. Transformation bacteria heat chock and incubation? - (reply: 2)
  93. competent cells - (reply: 2)
  94. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  95. transformation fails, bacteria grows in control plate - (reply: 6)
  96. Transient Transformation of Nicotiana benthamiana leaves - (reply: 1)
  97. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  98. transformation of green algae - agrobac.mediated transformation... (reply: 3)
  99. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
  100. competent cells stock - (reply: 3)
  101. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
  102. ligation and transformation problem - (reply: 1)
  103. growing competent cells - (reply: 1)
  104. making more competent cells - (reply: 2)
  105. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
  106. Transformation & Minimal Plates - (reply: 5)
  107. Transformation issue - (reply: 1)
  108. yeast transformation - (reply: 1)
  109. Problems with ligation and transformation - (reply: 2)
  110. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  111. Reasons for failure of transformation - (reply: 11)
  112. Small Colonies After Transformation - (reply: 5)
  113. Ligation and transformation problem - (reply: 2)
  114. Agrobacterium EHA101 competent cells - (reply: 1)
  115. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
  116. Chemical competent E.coli always from single colony ? - (reply: 2)
  117. No colonies after transformation! - (reply: 2)
  118. questions about transformation - (reply: 4)
  119. Trouble with growth of transformed cells - (reply: 4)
  120. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
  121. Transformants Don't Grow - (reply: 4)
  122. TERT transformation of fibroblast cells - Immortilising fibroblast cells. Which companies available? (reply: 1)
  123. Freezing home-brewed competent cells - Dry-ice first or straight to -80 C freezer? (reply: 6)
  124. DH5 Alpha Transformation - (reply: 2)
  125. TSS Transformation troubleshooting - no colonies on plate (reply: 4)
  126. Starting cells for making one's own competent cells - (reply: 7)
  127. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  128. double transformations - (reply: 1)
  129. Unusual band in transformants - (reply: 2)
  130. qPCR for transformed cells - (reply: 1)
  131. Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
  132. Ligation with EcorV and PacI - Ligation and transformation! (reply: 8)
  133. H-ras transformed RK3E/tv-a cells - (reply: 1)
  134. Competent cell storage for long term use - (reply: 2)
  135. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
  136. Why does the amount of plamids transformed affect colony count? - (reply: 2)
  137. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  138. competent cells - OD600 reading (reply: 6)
  139. grow in broth but not on agar... - after transformation (reply: 2)
  140. verifying a positive transformant - (reply: 3)
  141. transformation efficiency - (reply: 7)
  142. transformed bacteria doesnt grow - (reply: 1)
  143. Transformation background problem - high background in negative control (reply: 9)
  144. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  145. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  146. What is the role of DMSO in yeast transformation - (reply: 1)
  147. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  148. Lactobacillus electro-transformation - Lactobacillus wildtype transformation problem (reply: 4)
  149. Transformation(?) issues - (reply: 3)
  150. ligation/transformation failed - (reply: 7)
  151. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
  152. BL21(DE3)LysS competent cells - (reply: 1)
  153. insert in the reverse orientation after transformation - (reply: 5)
  154. Biolistic (transformation efficiency) - (reply: 2)
  155. CaCl2 for making competent cells - (reply: 3)
  156. Extremely small bacterial colonies after transformation - (reply: 10)
  157. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
  158. help with transformation efficiency calculation! - (reply: 2)
  159. help with transformation efficiency calculation! - (reply: 1)
  160. dh5 alpha competent cells? - (reply: 2)
  161. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
  162. pre-prepararation of competent cells - (reply: 3)
  163. point mutation when transformed in BL21 - (reply: 2)
  164. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
  165. Screening colonies after transformation - (reply: 2)
  166. z competent cells - (reply: 2)
  167. macrophage transformation - (reply: 1)
  168. PIR 1 Competent Cells - Poor Transformation Efficiency (reply: 1)
  169. have problems with electroporation transformation of PCP20 into BL21 de3 - (reply: 2)
  170. E coli transformation - (reply: 8)
  171. problem with yeast transformation - (reply: 2)
  172. Making competent cell from freeze-thawed stock - (reply: 2)
  173. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  174. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
  175. Bacteria transformation - (reply: 1)
  176. Transformation and ligation not working!!!!!!! - funny results please help (reply: 9)
  177. Chosing the right competent cells - (reply: 1)
  178. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  179. ccdB survival E. coli transformation - (reply: 2)
  180. Why, oh why, am I having so much trouble with ligations and transformations? - I need a little holiday help (reply: 4)
  181. cempetent e.coli and transformation - (reply: 2)
  182. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
  183. Yeast transformation - (reply: 1)
  184. FUNGI SILENCING - PROTOCOL TO TRANSFORM FUNGI CELLS (reply: 2)
  185. transformation medium - (reply: 2)
  186. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  187. same weird weird staph transformation results - any idea what is going on? (reply: 12)
  188. Picking strain for e coli competent cells - (reply: 6)
  189. is too much ligation reaction bad for transformation? - (reply: 4)
  190. please help!!! with transformation - (reply: 10)
  191. Failed Transformation of Tuner (DE3) cells - (reply: 1)
  192. Concentration of DNA for Transformation - (reply: 1)
  193. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  194. Non specific yeast transformations (or none at all) - (reply: 1)
  195. competent cell preparation? - (reply: 1)
  196. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  197. Competent Cells - (reply: 5)
  198. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  199. Mutation during transformation/maxiprep - (reply: 1)
  200. COMPETENT CELL PREPARATION - (reply: 6)
  201. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
  202. Transformation problem. Please help! - 1.7kb insert to 5.4kb vector; double digested w/ EcoRV & XhoI (reply: 17)
  203. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  204. Transformant plating advise - How long can I store my transofrmants at 4'C? (reply: 9)
  205. PCR problem from transformed TOPO TA vector - (reply: 3)
  206. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  207. calculating the mean change in a log transformed variable - is it possible? (reply: 1)
  208. transformation problem - (reply: 3)
  209. PCR screening of transformed bacterial colony - (reply: 6)
  210. Growing of TOP10 chemically competent cells - (reply: 4)
  211. Transform staph? - (reply: 2)
  212. YOU SHALL NOT PASS!! - bacteria will not transform..I think:/ (reply: 4)
  213. Help with transformation - (reply: 3)
  214. "false transformants" from B. subtilis transformation - how to get rid of them? (reply: 3)
  215. Ligation/Transformation Problems 9I'm not sure which!) - (reply: 3)
  216. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  217. help with ligation/transformation - (reply: 1)
  218. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
  219. help with transformation! thanks!! - (reply: 15)
  220. ligation - didn't got transformed colonies (reply: 1)
  221. Troubleshooting Digest, ligation, transformation - (reply: 7)
  222. Competent DH5 alpha - (reply: 4)
  223. what is co transformation scientificaly ? - what is co transformation scientificaly ? (reply: 1)
  224. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
  225. Chemically competent to electrocompetent DH5alpha - (reply: 1)
  226. To what extent competent cells be thawed before transformation? - (reply: 4)
  227. contamination in transformed bacteria - black filamentous fungi grows when culturing competent bacteria with m (reply: 8)
  228. Transformation question - (reply: 9)
  229. log transformed data - still not normal (reply: 2)
  230. making competent E coli DH5a cells - (reply: 6)