DH5 Alpha Transformation - (Jan/23/2011 )
I actually have a problem.
I transformed the DH5 alpha.
It was supposed to become luminescent and not grow on amp plates.
My strain did?
Any suggestions to this problem?
I don't understand. What did you transform them with? Did you have an insert disrupting an ampicillin resistance gene? Otherwise, why would you try to grow your transformed bacteria on a medium which (you thought) would kill them?
DH5 alpha doenst have an amp resistance gene...
How can you make it resistance then without adding this gene by introducing a plasmid or transformation of the DNA with this gene.