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Purification Related Discussions
  1. Plasmid purification for Gibson Assembly - (reply: 2)
  2. HSP70 contamination after Nickel purification - (reply: 3)
  3. Doubt on HisTag protein purification miniprep - (reply: 3)
  4. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
  5. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
  6. Affinity purification glycine elution chemistry - (reply: 3)
  7. storage conditions for purified protein - (reply: 2)
  8. large scale protein purification - (reply: 5)
  9. Gel Purification: When is it safe to pause? - (reply: 2)
  10. after protein purification - (reply: 4)
  11. Who can advise me a kit that would allow me to purify amplicons from quantitativ - (reply: 3)
  12. Cloning purified PCR product into cells - (reply: 5)
  13. antibody/DNAconjugation and purification - (reply: 1)
  14. Affinity Purification of 5′ Capped mRNA by Using High-Affinity GST-4E - (reply: 1)
  15. Question about MBP purification - (reply: 2)
  16. RNA purification with TRI_ low yield - (reply: 1)
  17. Why Iím not getting any band after column purification? - (reply: 1)
  18. Proteinase extraction and purification - (reply: 1)
  19. Protocols to identify or purify a bacterial beta-hemolysin from blood agar? - (reply: 4)
  20. Purification of metallothioneins? - (reply: 1)
  21. Low 260/230 ratios from DNA purification question - (reply: 1)
  22. ChIP: Inconsistent reverse crosslinking and DNA purification result - (reply: 2)
  23. Please help me :Protein expression and purification - (reply: 1)
  24. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
  25. Bacterial DNA extraction/purification from animal tissue - (reply: 1)
  26. DNA virus purification from agarose failed! - (reply: 7)
  27. Any real difference between DNA purification kits? - (reply: 9)
  28. how much purify protein nedded for IP - (reply: 9)
  29. regarding protein purification using 8m urea and sds page - (reply: 2)
  30. Protein Purification from mammalian cells - (reply: 1)
  31. Which method for DNA purification b4 cloning - (reply: 4)
  32. Purification for TOPO - (reply: 1)
  33. Purified DNA fragments are bigger than unpurified - (reply: 2)
  34. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
  35. his-tag purification - (reply: 9)
  36. IgG purification from hybridomas - (reply: 2)
  37. DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
  38. faint dna band after pcr purification - (reply: 1)
  39. transformation and protein purification calculation help; very basic - (reply: 5)
  40. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  41. Plasmid digestion and gel purification using HindIII - (reply: 3)
  42. Do protein purification under partially denatured condition - (reply: 1)
  43. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
  44. Gel purification (DNA) - (reply: 1)
  45. Viral contamination in DNA purification columns - (reply: 1)
  46. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  47. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  48. Protein Purification-catastrophe while purifying? - (reply: 2)
  49. What is the membrane made of in DNA purification kits - (reply: 3)
  50. Protein purification for adherent mammalian cells - (reply: 2)
  51. pGEX-2t purification problem - (reply: 3)
  52. Problem with strep tagged protein purification - (reply: 5)
  53. protein purification in acanthamoeba lysate - (reply: 3)
  54. Polyclonal Ab purification - (reply: 1)
  55. purify protein from native gel - (reply: 1)
  56. purifying proteins with repeats - (reply: 3)
  57. PCR RFLP is PCR product purification necessary? - (reply: 3)
  58. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  59. Protein purification from greenish supernatant of Pichia pastoris - (reply: 10)
  60. Streptavidin purification in the lab - (reply: 4)
  61. adding Tween80 to bacterial culture for protein purification ?! - (reply: 3)
  62. Phenol Chloroform Isoamyl Alcohol (25:24:21) DNA purification - (reply: 3)
  63. No DNA after PCR product purification - (reply: 9)
  64. removal of ethanol in PCR product purification - (reply: 5)
  65. Trouble with purified recombinant protein bands - (reply: 3)
  66. Protein activation after purification - (reply: 4)
  67. Contaminants in protein purification.....help! - (reply: 3)
  68. Protein expression and purification - Please help - (reply: 2)
  69. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  70. tag for protein purification - (reply: 7)
  71. Low yield PCR product after gel purification - (reply: 8)
  72. Trouble in GST- fusion protein purification - (reply: 1)
  73. low concentration protein purification - (reply: 1)
  74. Calculating amount of Beads for purification of Ig - (reply: 3)
  75. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  76. PCR product purification - (reply: 4)
  77. EMSA: cell extract vs purified protein - (reply: 2)
  78. Protein purification method to isolate elicitor with biological activity - (reply: 1)
  79. purification and binding of protein in denaturing conditions - (reply: 2)
  80. problem with Enzyme purification - (reply: 4)
  81. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
  82. Protein Purification from Polyacrylamide Gels - (reply: 3)
  83. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  84. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  85. Purifying a "protein" - (reply: 2)
  86. Help! Purifying native protein-2 years and counting... - (reply: 4)
  87. Large scale Viral RNA purification Kit - (reply: 3)
  88. phage lysate purification - (reply: 2)
  89. the virus particles purification methods - (reply: 7)
  90. Silanized equipment when purifying tRNA? - (reply: 1)
  91. Plasmid Purification and Digestion Problem - (reply: 2)
  92. Purification of FC tagged proteins - (reply: 3)
  93. Hypo-osmotic lysisbuffer for "purification" of mitochondria. - (reply: 5)
  94. GST fusion protein purification - (reply: 2)
  95. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
  96. pcr purification - (reply: 1)
  97. pcr purification doubt - (reply: 4)
  98. Mouse IgG1 purification - Protein G - (reply: 1)
  99. How to purify antioxidants enzymes from plant tissue. - (reply: 1)
  100. pcr purification - (reply: 1)
  101. Use PCR purification kit to purify restriction digestion - (reply: 2)
  102. Help with His-Tag protein purification - (reply: 2)
  103. protien purification using flag affinity - (reply: 4)
  104. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
  105. MW of PEG for Purification of DNA - (reply: 2)
  106. HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
  107. Reversed-Phase chromatography for protein purification - (reply: 3)
  108. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  109. problem with purification of gst-tagged protein - (reply: 2)
  110. Saliva and GCF DNA purification - (reply: 1)
  111. DNA isolation/purification troubleshooting. - (reply: 5)
  112. How to purify RNA from a handful number of cells? - (reply: 5)
  113. RNA purification from urine samples - (reply: 2)
  114. Purifying recombinant protein from pellet? - (reply: 2)
  115. Purifying RNA from Solution with RNEasy? - (reply: 2)
  116. Columns for ssDNA oligo purification - (reply: 2)
  117. low concentration of purified PCR product - (reply: 2)
  118. 5' RACE cDNA Concentration After SNAP Purification? - (reply: 3)
  119. what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
  120. Extra bands after protein expression/purification - (reply: 4)
  121. PCRed on restriction sites -- how do I purify it? - (reply: 8)
  122. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  123. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  124. Troubleshooting for DNA purification - (reply: 1)
  125. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  126. Insoluble Protein purification - (reply: 1)
  127. How to purify unstable protein? - (reply: 10)
  128. Purification of oligos for EMSA - (reply: 1)
  129. Hisx18 purification - (reply: 4)
  130. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  131. getting rid of imidazole in purified protein? - (reply: 2)
  132. BN-PAGE of purified membrane associated protein - (reply: 5)
  133. Anion exchange purification - (reply: 3)
  134. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  135. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  136. LDH purification - (reply: 1)
  137. NO yield after purification using Qiagen kits - Please help! - (reply: 1)
  138. A problem with IgG purification by Protein G chromatography - (reply: 4)
  139. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  140. Help: I am loosing the DNA during PCR purification - (reply: 7)
  141. FACS Sorting and Protein Purification - (reply: 5)
  142. His-Taq purification - (reply: 4)
  143. Protein purification buffer - (reply: 3)
  144. Protein purification in native conditions - (reply: 1)
  145. Butyl-agarose/sepharose purification - (reply: 1)
  146. methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
  147. type of IgG in my sample?? - proteinA purified sample (reply: 1)
  148. Help with small protein with his-tag purification - (reply: 7)
  149. Purification of DNA after MeDIP and WGA - (reply: 1)
  150. Protein purification from HEK 293 cells - (reply: 1)
  151. ~200kb Plasmid DNA purification is not working - (reply: 1)
  152. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  153. protein purification - (reply: 1)
  154. plant protein purification opinion - (reply: 3)
  155. Unable to Purify protein! - Protein purification (reply: 2)
  156. Problem with Pierce GST spin purification column - (reply: 6)
  157. HIS-tagged protein purification - (reply: 12)
  158. His-tagged protein purification problem - (reply: 2)
  159. Inclusion body purification - (reply: 1)
  160. Purification of recombinant proteins from insect cells - (reply: 2)
  161. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
  162. No DNA after PCR purification with QiaQuick? - (reply: 4)
  163. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  164. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
  165. Multiple bands from purified PCR product - (reply: 3)
  166. Troubleshooting Protein Purification using FPLC - (reply: 1)
  167. protein yield is very low after purification - protein purification (reply: 9)
  168. His tag protein purification - (reply: 7)
  169. Help with GST protein purification - (reply: 4)
  170. Purification of His tagged protein - (reply: 2)
  171. Protein purification. How to make function of recombinant protein? - (reply: 2)
  172. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
  173. purification of PCR product for cloning in a vector - (reply: 3)
  174. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
  175. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  176. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
  177. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  178. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  179. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  180. Decrease in concentration after protein purification - (reply: 3)
  181. inactive enzyme from purification - (reply: 1)
  182. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
  183. His-tagged protein purification - (reply: 3)
  184. protein purification - (reply: 3)
  185. Purification od insoluble protein (TIMP3) - (reply: 2)
  186. white residue after DNA purification - (reply: 3)
  187. Problems with qiaquick gel purification kit - cloning (reply: 10)
  188. problem in protein purification - (reply: 5)
  189. protein purification - (reply: 5)
  190. Help Me With Polyclonal Production - Rabbit Serum Purification (reply: 8)
  191. Problem with protein purification By FPLC - (reply: 4)
  192. uncut vector contamination in gel purified band - (reply: 2)
  193. Purify Chlorella Sample - (reply: 3)
  194. Purified lambda phages - (reply: 1)
  195. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  196. DNA purification from blood - (reply: 6)
  197. Preparation of Purified Inclusion Bodies - (reply: 2)
  198. salt for DNA precipitation - DNA purification (reply: 3)
  199. DNA quantification after gel purification - (reply: 6)
  200. Protein Purification by precipitation - (reply: 2)
  201. Problem with MBP and GST tag purification - (reply: 1)
  202. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
  203. Protein TAG for affinity purification - (reply: 1)
  204. M2 Flag purification - (reply: 2)
  205. Recombinant protein aggregation after purification - (reply: 2)
  206. best and simple method to purify protein - (reply: 38)
  207. strange problem with His purification - (reply: 1)
  208. ChIP purification method - (reply: 7)
  209. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
  210. antibody purification - ammonium sulfate precipitation - (reply: 7)
  211. Problem with Histag purification - (reply: 1)
  212. How to use promaga invitro translated pr. for Pierce EMSA? - translated Pr. purify needed? (reply: 3)
  213. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
  214. IMPACT system purification problem - (reply: 1)
  215. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  216. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
  217. Purification of Protein Extract for ELISA - (reply: 1)
  218. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  219. Help...purify and concentrate protein for IEF - (reply: 1)
  220. NI-NTA purification - (reply: 2)
  221. What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
  222. DNA purification - DNA purification dissolved in water (reply: 3)
  223. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  224. Purification problems his-tag - I have tried everything (reply: 3)
  225. weak purified antibody - (reply: 4)
  226. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
  227. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  228. His Tag Protein Purification - (reply: 6)
  229. Inclusion Body purification - (reply: 2)
  230. membrane protein purification - (reply: 6)
  231. Inseparable insert in gel purification of the vector - (reply: 3)
  232. Contamination in GST fusion protein purification - (reply: 2)
  233. Purification of scretory protein from bacteria - (reply: 2)
  234. Ni-NTA his tag protein purification - (reply: 1)
  235. Tips for antibody purification from crude serum? - (reply: 3)
  236. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
  237. Problems with affinity purification with M2 beads - (reply: 2)
  238. plasmid purification kit for transfection - (reply: 2)
  239. best plasmid purification kit for transfection - (reply: 1)
  240. Purify ChIP DNA using Qiagen spin filter - (reply: 8)
  241. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  242. problem in the GST fusion protein purification - (reply: 4)
  243. Troubleshooting GST purification - (reply: 4)
  244. Storing Purified Proteins - Seeking a Preservative (reply: 6)
  245. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  246. purification of a large amount of DNA - (reply: 3)
  247. Further DNA purification steps needed - Post DNAeasy Kit Purification (reply: 4)
  248. affinity purification and proteomics - (reply: 1)
  249. protein is degraded after purification - (reply: 2)
  250. problem in pcr and purification - (reply: 3)
  251. exosome purification - (reply: 2)
  252. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  253. PCR product purification - (reply: 3)
  254. Protein purification from Insect media - (reply: 1)
  255. Purifying a strange native protein - (reply: 6)
  256. Purification of virus particles - (reply: 2)
  257. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  258. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
  259. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  260. Couple questions about GST purification - (reply: 16)
  261. Rabbit IgG purification - Rabbit IgG purification (reply: 5)
  262. Trypsin Expression and Purification - (reply: 3)
  263. Purifying and cloning 69nt product - (reply: 5)
  264. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  265. dnase treatment before his-tag purification - (reply: 3)
  266. vortexing after phenol-chloroform-IMA purification - (reply: 3)
  267. His tag protein purification - (reply: 3)
  268. GST-fusion protein purification - How to purify it? (reply: 9)
  269. Gel Purification problem..Help~! - (reply: 7)
  270. mAb purification but precipitated - (reply: 13)
  271. Ab purification - (reply: 1)
  272. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  273. Problems in His-tag protein purification with TALON - (reply: 1)
  274. Endogenous protein purification - (reply: 2)
  275. purifying MMP9 - tagging sepharose beads with gelatin (reply: 1)
  276. Ask for help about the RNA purification protocol by phenol/chlorform methods - Ask for help about the RNA purification protocol by phenol/chlorform m (reply: 2)
  277. PCR and gel purification problem - did I screw this up (reply: 4)
  278. Purifying hydrogen bonded peripheral protein - (reply: 3)
  279. GST-tag protein purification - GST-tag protein purification (reply: 4)
  280. Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
  281. How to purify a transmembrane protein from others proteins? - (reply: 1)
  282. SDS Page Help - using purified protein (reply: 3)
  283. GST fusion protein purification - (reply: 1)
  284. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  285. B-per GST fusion protein purification kit - (reply: 2)
  286. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  287. Purifying protein with Neutravidin binding peptide tag - Protein purification (reply: 6)
  288. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
  289. how to concentrate/purify virus without ultracentrifugation? - (reply: 5)
  290. a doubt about PCR gel purification - (reply: 4)
  291. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
  292. Problem with DNA purification from agarose gel - (reply: 3)
  293. DNA purification - long fragments wanted - (reply: 1)
  294. purification of a basic protein - problem in elution (reply: 4)
  295. stabilization of purified protein solutions - (reply: 2)
  296. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  297. silica purification - problem with double bands after silica purification (reply: 1)
  298. Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
  299. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
  300. Gel purification of vector DNA - low yield - (reply: 26)
  301. gel purification or ligation problem - (reply: 3)
  302. Help with Purifying DNA Hairpin - (reply: 2)
  303. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  304. protein purification - (reply: 5)