Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Purification Related Discussions
  1. Please help me :Protein expression and purification - (reply: 1)
  2. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
  3. Bacterial DNA extraction/purification from animal tissue - (reply: 1)
  4. DNA virus purification from agarose failed! - (reply: 7)
  5. Any real difference between DNA purification kits? - (reply: 9)
  6. how much purify protein nedded for IP - (reply: 9)
  7. regarding protein purification using 8m urea and sds page - (reply: 2)
  8. Protein Purification from mammalian cells - (reply: 1)
  9. Which method for DNA purification b4 cloning - (reply: 4)
  10. Purification for TOPO - (reply: 1)
  11. Purified DNA fragments are bigger than unpurified - (reply: 2)
  12. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
  13. his-tag purification - (reply: 9)
  14. IgG purification from hybridomas - (reply: 2)
  15. DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
  16. transformation and protein purification calculation help; very basic - (reply: 5)
  17. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  18. Plasmid digestion and gel purification using HindIII - (reply: 3)
  19. Do protein purification under partially denatured condition - (reply: 1)
  20. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
  21. Gel purification (DNA) - (reply: 1)
  22. Viral contamination in DNA purification columns - (reply: 1)
  23. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  24. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  25. Protein Purification-catastrophe while purifying? - (reply: 2)
  26. What is the membrane made of in DNA purification kits - (reply: 3)
  27. Protein purification for adherent mammalian cells - (reply: 2)
  28. pGEX-2t purification problem - (reply: 3)
  29. Problem with strep tagged protein purification - (reply: 5)
  30. protein purification in acanthamoeba lysate - (reply: 3)
  31. Polyclonal Ab purification - (reply: 1)
  32. purify protein from native gel - (reply: 1)
  33. purifying proteins with repeats - (reply: 3)
  34. PCR RFLP is PCR product purification necessary? - (reply: 3)
  35. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  36. Protein purification from greenish supernatant of Pichia pastoris - (reply: 10)
  37. Streptavidin purification in the lab - (reply: 4)
  38. adding Tween80 to bacterial culture for protein purification ?! - (reply: 3)
  39. Phenol Chloroform Isoamyl Alcohol (25:24:21) DNA purification - (reply: 3)
  40. No DNA after PCR product purification - (reply: 9)
  41. removal of ethanol in PCR product purification - (reply: 5)
  42. Trouble with purified recombinant protein bands - (reply: 3)
  43. Protein activation after purification - (reply: 4)
  44. Contaminants in protein purification.....help! - (reply: 3)
  45. Protein expression and purification - Please help - (reply: 2)
  46. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  47. tag for protein purification - (reply: 7)
  48. Low yield PCR product after gel purification - (reply: 8)
  49. Trouble in GST- fusion protein purification - (reply: 1)
  50. low concentration protein purification - (reply: 1)
  51. Calculating amount of Beads for purification of Ig - (reply: 3)
  52. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  53. PCR product purification - (reply: 4)
  54. EMSA: cell extract vs purified protein - (reply: 2)
  55. Protein purification method to isolate elicitor with biological activity - (reply: 1)
  56. purification and binding of protein in denaturing conditions - (reply: 2)
  57. problem with Enzyme purification - (reply: 4)
  58. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
  59. Protein Purification from Polyacrylamide Gels - (reply: 3)
  60. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  61. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  62. Purifying a "protein" - (reply: 2)
  63. Help! Purifying native protein-2 years and counting... - (reply: 4)
  64. Large scale Viral RNA purification Kit - (reply: 3)
  65. phage lysate purification - (reply: 2)
  66. the virus particles purification methods - (reply: 7)
  67. Silanized equipment when purifying tRNA? - (reply: 1)
  68. Plasmid Purification and Digestion Problem - (reply: 2)
  69. Purification of FC tagged proteins - (reply: 3)
  70. Hypo-osmotic lysisbuffer for "purification" of mitochondria. - (reply: 5)
  71. GST fusion protein purification - (reply: 2)
  72. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
  73. pcr purification - (reply: 1)
  74. pcr purification doubt - (reply: 4)
  75. Mouse IgG1 purification - Protein G - (reply: 1)
  76. How to purify antioxidants enzymes from plant tissue. - (reply: 1)
  77. pcr purification - (reply: 1)
  78. Use PCR purification kit to purify restriction digestion - (reply: 2)
  79. Help with His-Tag protein purification - (reply: 2)
  80. protien purification using flag affinity - (reply: 4)
  81. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
  82. MW of PEG for Purification of DNA - (reply: 2)
  83. HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
  84. Reversed-Phase chromatography for protein purification - (reply: 3)
  85. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  86. problem with purification of gst-tagged protein - (reply: 2)
  87. Saliva and GCF DNA purification - (reply: 1)
  88. DNA isolation/purification troubleshooting. - (reply: 5)
  89. How to purify RNA from a handful number of cells? - (reply: 5)
  90. RNA purification from urine samples - (reply: 2)
  91. Purifying recombinant protein from pellet? - (reply: 2)
  92. Purifying RNA from Solution with RNEasy? - (reply: 2)
  93. Columns for ssDNA oligo purification - (reply: 2)
  94. low concentration of purified PCR product - (reply: 2)
  95. 5' RACE cDNA Concentration After SNAP Purification? - (reply: 3)
  96. what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
  97. Extra bands after protein expression/purification - (reply: 4)
  98. PCRed on restriction sites -- how do I purify it? - (reply: 8)
  99. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  100. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  101. Troubleshooting for DNA purification - (reply: 1)
  102. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  103. Insoluble Protein purification - (reply: 1)
  104. How to purify unstable protein? - (reply: 10)
  105. Purification of oligos for EMSA - (reply: 1)
  106. Hisx18 purification - (reply: 4)
  107. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  108. getting rid of imidazole in purified protein? - (reply: 2)
  109. BN-PAGE of purified membrane associated protein - (reply: 5)
  110. Anion exchange purification - (reply: 3)
  111. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  112. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  113. LDH purification - (reply: 1)
  114. NO yield after purification using Qiagen kits - Please help! - (reply: 1)
  115. A problem with IgG purification by Protein G chromatography - (reply: 4)
  116. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  117. Help: I am loosing the DNA during PCR purification - (reply: 7)
  118. FACS Sorting and Protein Purification - (reply: 5)
  119. His-Taq purification - (reply: 4)
  120. Protein purification buffer - (reply: 3)
  121. Protein purification in native conditions - (reply: 1)
  122. Butyl-agarose/sepharose purification - (reply: 1)
  123. methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
  124. type of IgG in my sample?? - proteinA purified sample (reply: 1)
  125. Help with small protein with his-tag purification - (reply: 7)
  126. Purification of DNA after MeDIP and WGA - (reply: 1)
  127. Protein purification from HEK 293 cells - (reply: 1)
  128. ~200kb Plasmid DNA purification is not working - (reply: 1)
  129. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  130. protein purification - (reply: 1)
  131. plant protein purification opinion - (reply: 3)
  132. Unable to Purify protein! - Protein purification (reply: 2)
  133. Problem with Pierce GST spin purification column - (reply: 6)
  134. HIS-tagged protein purification - (reply: 12)
  135. His-tagged protein purification problem - (reply: 2)
  136. Inclusion body purification - (reply: 1)
  137. Purification of recombinant proteins from insect cells - (reply: 2)
  138. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
  139. No DNA after PCR purification with QiaQuick? - (reply: 4)
  140. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  141. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
  142. Multiple bands from purified PCR product - (reply: 3)
  143. Troubleshooting Protein Purification using FPLC - (reply: 1)
  144. protein yield is very low after purification - protein purification (reply: 9)
  145. His tag protein purification - (reply: 7)
  146. Help with GST protein purification - (reply: 4)
  147. Purification of His tagged protein - (reply: 2)
  148. Protein purification. How to make function of recombinant protein? - (reply: 2)
  149. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
  150. purification of PCR product for cloning in a vector - (reply: 3)
  151. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
  152. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  153. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
  154. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  155. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  156. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  157. Decrease in concentration after protein purification - (reply: 3)
  158. inactive enzyme from purification - (reply: 1)
  159. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
  160. His-tagged protein purification - (reply: 3)
  161. protein purification - (reply: 3)
  162. Purification od insoluble protein (TIMP3) - (reply: 2)
  163. white residue after DNA purification - (reply: 3)
  164. Problems with qiaquick gel purification kit - cloning (reply: 10)
  165. problem in protein purification - (reply: 5)
  166. protein purification - (reply: 5)
  167. Help Me With Polyclonal Production - Rabbit Serum Purification (reply: 8)
  168. Problem with protein purification By FPLC - (reply: 4)
  169. uncut vector contamination in gel purified band - (reply: 2)
  170. Purify Chlorella Sample - (reply: 3)
  171. Purified lambda phages - (reply: 1)
  172. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  173. DNA purification from blood - (reply: 6)
  174. Preparation of Purified Inclusion Bodies - (reply: 2)
  175. salt for DNA precipitation - DNA purification (reply: 3)
  176. DNA quantification after gel purification - (reply: 6)
  177. Protein Purification by precipitation - (reply: 2)
  178. Problem with MBP and GST tag purification - (reply: 1)
  179. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
  180. Protein TAG for affinity purification - (reply: 1)
  181. M2 Flag purification - (reply: 2)
  182. Recombinant protein aggregation after purification - (reply: 2)
  183. best and simple method to purify protein - (reply: 38)
  184. strange problem with His purification - (reply: 1)
  185. ChIP purification method - (reply: 7)
  186. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
  187. antibody purification - ammonium sulfate precipitation - (reply: 7)
  188. Problem with Histag purification - (reply: 1)
  189. How to use promaga invitro translated pr. for Pierce EMSA? - translated Pr. purify needed? (reply: 3)
  190. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
  191. IMPACT system purification problem - (reply: 1)
  192. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  193. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
  194. Purification of Protein Extract for ELISA - (reply: 1)
  195. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  196. Help...purify and concentrate protein for IEF - (reply: 1)
  197. NI-NTA purification - (reply: 2)
  198. What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
  199. DNA purification - DNA purification dissolved in water (reply: 3)
  200. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  201. Purification problems his-tag - I have tried everything (reply: 3)
  202. weak purified antibody - (reply: 4)
  203. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
  204. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  205. His Tag Protein Purification - (reply: 6)
  206. Inclusion Body purification - (reply: 2)
  207. membrane protein purification - (reply: 6)
  208. Inseparable insert in gel purification of the vector - (reply: 3)
  209. Contamination in GST fusion protein purification - (reply: 2)
  210. Purification of scretory protein from bacteria - (reply: 2)
  211. Ni-NTA his tag protein purification - (reply: 1)
  212. Tips for antibody purification from crude serum? - (reply: 3)
  213. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
  214. Problems with affinity purification with M2 beads - (reply: 2)
  215. plasmid purification kit for transfection - (reply: 2)
  216. best plasmid purification kit for transfection - (reply: 1)
  217. Purify ChIP DNA using Qiagen spin filter - (reply: 6)
  218. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  219. problem in the GST fusion protein purification - (reply: 4)
  220. Troubleshooting GST purification - (reply: 4)
  221. Storing Purified Proteins - Seeking a Preservative (reply: 6)
  222. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  223. purification of a large amount of DNA - (reply: 3)
  224. Further DNA purification steps needed - Post DNAeasy Kit Purification (reply: 4)
  225. affinity purification and proteomics - (reply: 1)
  226. protein is degraded after purification - (reply: 2)
  227. problem in pcr and purification - (reply: 3)
  228. exosome purification - (reply: 2)
  229. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  230. PCR product purification - (reply: 3)
  231. Protein purification from Insect media - (reply: 1)
  232. Purifying a strange native protein - (reply: 6)
  233. Purification of virus particles - (reply: 2)
  234. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  235. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
  236. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  237. Couple questions about GST purification - (reply: 16)
  238. Rabbit IgG purification - Rabbit IgG purification (reply: 5)
  239. Trypsin Expression and Purification - (reply: 3)
  240. Purifying and cloning 69nt product - (reply: 5)
  241. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  242. dnase treatment before his-tag purification - (reply: 3)
  243. vortexing after phenol-chloroform-IMA purification - (reply: 3)
  244. His tag protein purification - (reply: 3)
  245. GST-fusion protein purification - How to purify it? (reply: 9)
  246. Gel Purification problem..Help~! - (reply: 7)
  247. mAb purification but precipitated - (reply: 13)
  248. Ab purification - (reply: 1)
  249. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  250. Problems in His-tag protein purification with TALON - (reply: 1)
  251. Endogenous protein purification - (reply: 2)
  252. purifying MMP9 - tagging sepharose beads with gelatin (reply: 1)
  253. Ask for help about the RNA purification protocol by phenol/chlorform methods - Ask for help about the RNA purification protocol by phenol/chlorform m (reply: 2)
  254. PCR and gel purification problem - did I screw this up (reply: 4)
  255. Purifying hydrogen bonded peripheral protein - (reply: 3)
  256. GST-tag protein purification - GST-tag protein purification (reply: 4)
  257. Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
  258. How to purify a transmembrane protein from others proteins? - (reply: 1)
  259. SDS Page Help - using purified protein (reply: 3)
  260. GST fusion protein purification - (reply: 1)
  261. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  262. B-per GST fusion protein purification kit - (reply: 2)
  263. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  264. Purifying protein with Neutravidin binding peptide tag - Protein purification (reply: 6)
  265. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
  266. how to concentrate/purify virus without ultracentrifugation? - (reply: 5)
  267. a doubt about PCR gel purification - (reply: 4)
  268. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
  269. Problem with DNA purification from agarose gel - (reply: 3)
  270. DNA purification - long fragments wanted - (reply: 1)
  271. purification of a basic protein - problem in elution (reply: 4)
  272. stabilization of purified protein solutions - (reply: 2)
  273. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  274. silica purification - problem with double bands after silica purification (reply: 1)
  275. Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
  276. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
  277. Gel purification of vector DNA - low yield - (reply: 26)
  278. gel purification or ligation problem - (reply: 3)
  279. Help with Purifying DNA Hairpin - (reply: 2)
  280. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  281. protein purification - (reply: 5)