Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Purification Related Discussions
  1. Why Iím not getting any band after column purification? - (reply: 1)
  2. Proteinase extraction and purification - (reply: 1)
  3. Protocols to identify or purify a bacterial beta-hemolysin from blood agar? - (reply: 1)
  4. Purification of metallothioneins? - (reply: 1)
  5. Low 260/230 ratios from DNA purification question - (reply: 1)
  6. ChIP: Inconsistent reverse crosslinking and DNA purification result - (reply: 2)
  7. Please help me :Protein expression and purification - (reply: 1)
  8. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
  9. Bacterial DNA extraction/purification from animal tissue - (reply: 1)
  10. DNA virus purification from agarose failed! - (reply: 7)
  11. Any real difference between DNA purification kits? - (reply: 9)
  12. how much purify protein nedded for IP - (reply: 9)
  13. regarding protein purification using 8m urea and sds page - (reply: 2)
  14. Protein Purification from mammalian cells - (reply: 1)
  15. Which method for DNA purification b4 cloning - (reply: 4)
  16. Purification for TOPO - (reply: 1)
  17. Purified DNA fragments are bigger than unpurified - (reply: 2)
  18. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
  19. his-tag purification - (reply: 9)
  20. IgG purification from hybridomas - (reply: 2)
  21. DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
  22. transformation and protein purification calculation help; very basic - (reply: 5)
  23. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  24. Plasmid digestion and gel purification using HindIII - (reply: 3)
  25. Do protein purification under partially denatured condition - (reply: 1)
  26. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
  27. Gel purification (DNA) - (reply: 1)
  28. Viral contamination in DNA purification columns - (reply: 1)
  29. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  30. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  31. Protein Purification-catastrophe while purifying? - (reply: 2)
  32. What is the membrane made of in DNA purification kits - (reply: 3)
  33. Protein purification for adherent mammalian cells - (reply: 2)
  34. pGEX-2t purification problem - (reply: 3)
  35. Problem with strep tagged protein purification - (reply: 5)
  36. protein purification in acanthamoeba lysate - (reply: 3)
  37. Polyclonal Ab purification - (reply: 1)
  38. purify protein from native gel - (reply: 1)
  39. purifying proteins with repeats - (reply: 3)
  40. PCR RFLP is PCR product purification necessary? - (reply: 3)
  41. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  42. Protein purification from greenish supernatant of Pichia pastoris - (reply: 10)
  43. Streptavidin purification in the lab - (reply: 4)
  44. adding Tween80 to bacterial culture for protein purification ?! - (reply: 3)
  45. Phenol Chloroform Isoamyl Alcohol (25:24:21) DNA purification - (reply: 3)
  46. No DNA after PCR product purification - (reply: 9)
  47. removal of ethanol in PCR product purification - (reply: 5)
  48. Trouble with purified recombinant protein bands - (reply: 3)
  49. Protein activation after purification - (reply: 4)
  50. Contaminants in protein purification.....help! - (reply: 3)
  51. Protein expression and purification - Please help - (reply: 2)
  52. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  53. tag for protein purification - (reply: 7)
  54. Low yield PCR product after gel purification - (reply: 8)
  55. Trouble in GST- fusion protein purification - (reply: 1)
  56. low concentration protein purification - (reply: 1)
  57. Calculating amount of Beads for purification of Ig - (reply: 3)
  58. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  59. PCR product purification - (reply: 4)
  60. EMSA: cell extract vs purified protein - (reply: 2)
  61. Protein purification method to isolate elicitor with biological activity - (reply: 1)
  62. purification and binding of protein in denaturing conditions - (reply: 2)
  63. problem with Enzyme purification - (reply: 4)
  64. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
  65. Protein Purification from Polyacrylamide Gels - (reply: 3)
  66. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  67. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  68. Purifying a "protein" - (reply: 2)
  69. Help! Purifying native protein-2 years and counting... - (reply: 4)
  70. Large scale Viral RNA purification Kit - (reply: 3)
  71. phage lysate purification - (reply: 2)
  72. the virus particles purification methods - (reply: 7)
  73. Silanized equipment when purifying tRNA? - (reply: 1)
  74. Plasmid Purification and Digestion Problem - (reply: 2)
  75. Purification of FC tagged proteins - (reply: 3)
  76. Hypo-osmotic lysisbuffer for "purification" of mitochondria. - (reply: 5)
  77. GST fusion protein purification - (reply: 2)
  78. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
  79. pcr purification - (reply: 1)
  80. pcr purification doubt - (reply: 4)
  81. Mouse IgG1 purification - Protein G - (reply: 1)
  82. How to purify antioxidants enzymes from plant tissue. - (reply: 1)
  83. pcr purification - (reply: 1)
  84. Use PCR purification kit to purify restriction digestion - (reply: 2)
  85. Help with His-Tag protein purification - (reply: 2)
  86. protien purification using flag affinity - (reply: 4)
  87. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
  88. MW of PEG for Purification of DNA - (reply: 2)
  89. HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
  90. Reversed-Phase chromatography for protein purification - (reply: 3)
  91. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  92. problem with purification of gst-tagged protein - (reply: 2)
  93. Saliva and GCF DNA purification - (reply: 1)
  94. DNA isolation/purification troubleshooting. - (reply: 5)
  95. How to purify RNA from a handful number of cells? - (reply: 5)
  96. RNA purification from urine samples - (reply: 2)
  97. Purifying recombinant protein from pellet? - (reply: 2)
  98. Purifying RNA from Solution with RNEasy? - (reply: 2)
  99. Columns for ssDNA oligo purification - (reply: 2)
  100. low concentration of purified PCR product - (reply: 2)
  101. 5' RACE cDNA Concentration After SNAP Purification? - (reply: 3)
  102. what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
  103. Extra bands after protein expression/purification - (reply: 4)
  104. PCRed on restriction sites -- how do I purify it? - (reply: 8)
  105. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  106. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  107. Troubleshooting for DNA purification - (reply: 1)
  108. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  109. Insoluble Protein purification - (reply: 1)
  110. How to purify unstable protein? - (reply: 10)
  111. Purification of oligos for EMSA - (reply: 1)
  112. Hisx18 purification - (reply: 4)
  113. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  114. getting rid of imidazole in purified protein? - (reply: 2)
  115. BN-PAGE of purified membrane associated protein - (reply: 5)
  116. Anion exchange purification - (reply: 3)
  117. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  118. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  119. LDH purification - (reply: 1)
  120. NO yield after purification using Qiagen kits - Please help! - (reply: 1)
  121. A problem with IgG purification by Protein G chromatography - (reply: 4)
  122. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  123. Help: I am loosing the DNA during PCR purification - (reply: 7)
  124. FACS Sorting and Protein Purification - (reply: 5)
  125. His-Taq purification - (reply: 4)
  126. Protein purification buffer - (reply: 3)
  127. Protein purification in native conditions - (reply: 1)
  128. Butyl-agarose/sepharose purification - (reply: 1)
  129. methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
  130. type of IgG in my sample?? - proteinA purified sample (reply: 1)
  131. Help with small protein with his-tag purification - (reply: 7)
  132. Purification of DNA after MeDIP and WGA - (reply: 1)
  133. Protein purification from HEK 293 cells - (reply: 1)
  134. ~200kb Plasmid DNA purification is not working - (reply: 1)
  135. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  136. protein purification - (reply: 1)
  137. plant protein purification opinion - (reply: 3)
  138. Unable to Purify protein! - Protein purification (reply: 2)
  139. Problem with Pierce GST spin purification column - (reply: 6)
  140. HIS-tagged protein purification - (reply: 12)
  141. His-tagged protein purification problem - (reply: 2)
  142. Inclusion body purification - (reply: 1)
  143. Purification of recombinant proteins from insect cells - (reply: 2)
  144. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
  145. No DNA after PCR purification with QiaQuick? - (reply: 4)
  146. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  147. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
  148. Multiple bands from purified PCR product - (reply: 3)
  149. Troubleshooting Protein Purification using FPLC - (reply: 1)
  150. protein yield is very low after purification - protein purification (reply: 9)
  151. His tag protein purification - (reply: 7)
  152. Help with GST protein purification - (reply: 4)
  153. Purification of His tagged protein - (reply: 2)
  154. Protein purification. How to make function of recombinant protein? - (reply: 2)
  155. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
  156. purification of PCR product for cloning in a vector - (reply: 3)
  157. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
  158. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  159. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
  160. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  161. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  162. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  163. Decrease in concentration after protein purification - (reply: 3)
  164. inactive enzyme from purification - (reply: 1)
  165. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
  166. His-tagged protein purification - (reply: 3)
  167. protein purification - (reply: 3)
  168. Purification od insoluble protein (TIMP3) - (reply: 2)
  169. white residue after DNA purification - (reply: 3)
  170. Problems with qiaquick gel purification kit - cloning (reply: 10)
  171. problem in protein purification - (reply: 5)
  172. protein purification - (reply: 5)
  173. Help Me With Polyclonal Production - Rabbit Serum Purification (reply: 8)
  174. Problem with protein purification By FPLC - (reply: 4)
  175. uncut vector contamination in gel purified band - (reply: 2)
  176. Purify Chlorella Sample - (reply: 3)
  177. Purified lambda phages - (reply: 1)
  178. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  179. DNA purification from blood - (reply: 6)
  180. Preparation of Purified Inclusion Bodies - (reply: 2)
  181. salt for DNA precipitation - DNA purification (reply: 3)
  182. DNA quantification after gel purification - (reply: 6)
  183. Protein Purification by precipitation - (reply: 2)
  184. Problem with MBP and GST tag purification - (reply: 1)
  185. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
  186. Protein TAG for affinity purification - (reply: 1)
  187. M2 Flag purification - (reply: 2)
  188. Recombinant protein aggregation after purification - (reply: 2)
  189. best and simple method to purify protein - (reply: 38)
  190. strange problem with His purification - (reply: 1)
  191. ChIP purification method - (reply: 7)
  192. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
  193. antibody purification - ammonium sulfate precipitation - (reply: 7)
  194. Problem with Histag purification - (reply: 1)
  195. How to use promaga invitro translated pr. for Pierce EMSA? - translated Pr. purify needed? (reply: 3)
  196. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
  197. IMPACT system purification problem - (reply: 1)
  198. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  199. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
  200. Purification of Protein Extract for ELISA - (reply: 1)
  201. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  202. Help...purify and concentrate protein for IEF - (reply: 1)
  203. NI-NTA purification - (reply: 2)
  204. What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
  205. DNA purification - DNA purification dissolved in water (reply: 3)
  206. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  207. Purification problems his-tag - I have tried everything (reply: 3)
  208. weak purified antibody - (reply: 4)
  209. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
  210. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  211. His Tag Protein Purification - (reply: 6)
  212. Inclusion Body purification - (reply: 2)
  213. membrane protein purification - (reply: 6)
  214. Inseparable insert in gel purification of the vector - (reply: 3)
  215. Contamination in GST fusion protein purification - (reply: 2)
  216. Purification of scretory protein from bacteria - (reply: 2)
  217. Ni-NTA his tag protein purification - (reply: 1)
  218. Tips for antibody purification from crude serum? - (reply: 3)
  219. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
  220. Problems with affinity purification with M2 beads - (reply: 2)
  221. plasmid purification kit for transfection - (reply: 2)
  222. best plasmid purification kit for transfection - (reply: 1)
  223. Purify ChIP DNA using Qiagen spin filter - (reply: 7)
  224. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  225. problem in the GST fusion protein purification - (reply: 4)
  226. Troubleshooting GST purification - (reply: 4)
  227. Storing Purified Proteins - Seeking a Preservative (reply: 6)
  228. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  229. purification of a large amount of DNA - (reply: 3)
  230. Further DNA purification steps needed - Post DNAeasy Kit Purification (reply: 4)
  231. affinity purification and proteomics - (reply: 1)
  232. protein is degraded after purification - (reply: 2)
  233. problem in pcr and purification - (reply: 3)
  234. exosome purification - (reply: 2)
  235. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  236. PCR product purification - (reply: 3)
  237. Protein purification from Insect media - (reply: 1)
  238. Purifying a strange native protein - (reply: 6)
  239. Purification of virus particles - (reply: 2)
  240. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  241. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
  242. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  243. Couple questions about GST purification - (reply: 16)
  244. Rabbit IgG purification - Rabbit IgG purification (reply: 5)
  245. Trypsin Expression and Purification - (reply: 3)
  246. Purifying and cloning 69nt product - (reply: 5)
  247. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  248. dnase treatment before his-tag purification - (reply: 3)
  249. vortexing after phenol-chloroform-IMA purification - (reply: 3)
  250. His tag protein purification - (reply: 3)
  251. GST-fusion protein purification - How to purify it? (reply: 9)
  252. Gel Purification problem..Help~! - (reply: 7)
  253. mAb purification but precipitated - (reply: 13)
  254. Ab purification - (reply: 1)
  255. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  256. Problems in His-tag protein purification with TALON - (reply: 1)
  257. Endogenous protein purification - (reply: 2)
  258. purifying MMP9 - tagging sepharose beads with gelatin (reply: 1)
  259. Ask for help about the RNA purification protocol by phenol/chlorform methods - Ask for help about the RNA purification protocol by phenol/chlorform m (reply: 2)
  260. PCR and gel purification problem - did I screw this up (reply: 4)
  261. Purifying hydrogen bonded peripheral protein - (reply: 3)
  262. GST-tag protein purification - GST-tag protein purification (reply: 4)
  263. Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
  264. How to purify a transmembrane protein from others proteins? - (reply: 1)
  265. SDS Page Help - using purified protein (reply: 3)
  266. GST fusion protein purification - (reply: 1)
  267. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  268. B-per GST fusion protein purification kit - (reply: 2)
  269. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  270. Purifying protein with Neutravidin binding peptide tag - Protein purification (reply: 6)
  271. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
  272. how to concentrate/purify virus without ultracentrifugation? - (reply: 5)
  273. a doubt about PCR gel purification - (reply: 4)
  274. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
  275. Problem with DNA purification from agarose gel - (reply: 3)
  276. DNA purification - long fragments wanted - (reply: 1)
  277. purification of a basic protein - problem in elution (reply: 4)
  278. stabilization of purified protein solutions - (reply: 2)
  279. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  280. silica purification - problem with double bands after silica purification (reply: 1)
  281. Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
  282. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
  283. Gel purification of vector DNA - low yield - (reply: 26)
  284. gel purification or ligation problem - (reply: 3)
  285. Help with Purifying DNA Hairpin - (reply: 2)
  286. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  287. protein purification - (reply: 5)