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Purification Related Discussions
  1. Doubt on HisTag protein purification miniprep - (reply: 3)
  2. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
  3. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
  4. Affinity purification glycine elution chemistry - (reply: 3)
  5. storage conditions for purified protein - (reply: 2)
  6. large scale protein purification - (reply: 5)
  7. Gel Purification: When is it safe to pause? - (reply: 2)
  8. after protein purification - (reply: 4)
  9. Who can advise me a kit that would allow me to purify amplicons from quantitativ - (reply: 3)
  10. Cloning purified PCR product into cells - (reply: 5)
  11. antibody/DNAconjugation and purification - (reply: 1)
  12. Affinity Purification of 5′ Capped mRNA by Using High-Affinity GST-4E - (reply: 1)
  13. Question about MBP purification - (reply: 2)
  14. RNA purification with TRI_ low yield - (reply: 1)
  15. Why Iím not getting any band after column purification? - (reply: 1)
  16. Proteinase extraction and purification - (reply: 1)
  17. Protocols to identify or purify a bacterial beta-hemolysin from blood agar? - (reply: 4)
  18. Purification of metallothioneins? - (reply: 1)
  19. Low 260/230 ratios from DNA purification question - (reply: 1)
  20. ChIP: Inconsistent reverse crosslinking and DNA purification result - (reply: 2)
  21. Please help me :Protein expression and purification - (reply: 1)
  22. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
  23. Bacterial DNA extraction/purification from animal tissue - (reply: 1)
  24. DNA virus purification from agarose failed! - (reply: 7)
  25. Any real difference between DNA purification kits? - (reply: 9)
  26. how much purify protein nedded for IP - (reply: 9)
  27. regarding protein purification using 8m urea and sds page - (reply: 2)
  28. Protein Purification from mammalian cells - (reply: 1)
  29. Which method for DNA purification b4 cloning - (reply: 4)
  30. Purification for TOPO - (reply: 1)
  31. Purified DNA fragments are bigger than unpurified - (reply: 2)
  32. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
  33. his-tag purification - (reply: 9)
  34. IgG purification from hybridomas - (reply: 2)
  35. DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
  36. faint dna band after pcr purification - (reply: 1)
  37. transformation and protein purification calculation help; very basic - (reply: 5)
  38. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  39. Plasmid digestion and gel purification using HindIII - (reply: 3)
  40. Do protein purification under partially denatured condition - (reply: 1)
  41. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
  42. Gel purification (DNA) - (reply: 1)
  43. Viral contamination in DNA purification columns - (reply: 1)
  44. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  45. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  46. Protein Purification-catastrophe while purifying? - (reply: 2)
  47. What is the membrane made of in DNA purification kits - (reply: 3)
  48. Protein purification for adherent mammalian cells - (reply: 2)
  49. pGEX-2t purification problem - (reply: 3)
  50. Problem with strep tagged protein purification - (reply: 5)
  51. protein purification in acanthamoeba lysate - (reply: 3)
  52. Polyclonal Ab purification - (reply: 1)
  53. purify protein from native gel - (reply: 1)
  54. purifying proteins with repeats - (reply: 3)
  55. PCR RFLP is PCR product purification necessary? - (reply: 3)
  56. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  57. Protein purification from greenish supernatant of Pichia pastoris - (reply: 10)
  58. Streptavidin purification in the lab - (reply: 4)
  59. adding Tween80 to bacterial culture for protein purification ?! - (reply: 3)
  60. Phenol Chloroform Isoamyl Alcohol (25:24:21) DNA purification - (reply: 3)
  61. No DNA after PCR product purification - (reply: 9)
  62. removal of ethanol in PCR product purification - (reply: 5)
  63. Trouble with purified recombinant protein bands - (reply: 3)
  64. Protein activation after purification - (reply: 4)
  65. Contaminants in protein purification.....help! - (reply: 3)
  66. Protein expression and purification - Please help - (reply: 2)
  67. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  68. tag for protein purification - (reply: 7)
  69. Low yield PCR product after gel purification - (reply: 8)
  70. Trouble in GST- fusion protein purification - (reply: 1)
  71. low concentration protein purification - (reply: 1)
  72. Calculating amount of Beads for purification of Ig - (reply: 3)
  73. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  74. PCR product purification - (reply: 4)
  75. EMSA: cell extract vs purified protein - (reply: 2)
  76. Protein purification method to isolate elicitor with biological activity - (reply: 1)
  77. purification and binding of protein in denaturing conditions - (reply: 2)
  78. problem with Enzyme purification - (reply: 4)
  79. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
  80. Protein Purification from Polyacrylamide Gels - (reply: 3)
  81. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  82. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  83. Purifying a "protein" - (reply: 2)
  84. Help! Purifying native protein-2 years and counting... - (reply: 4)
  85. Large scale Viral RNA purification Kit - (reply: 3)
  86. phage lysate purification - (reply: 2)
  87. the virus particles purification methods - (reply: 7)
  88. Silanized equipment when purifying tRNA? - (reply: 1)
  89. Plasmid Purification and Digestion Problem - (reply: 2)
  90. Purification of FC tagged proteins - (reply: 3)
  91. Hypo-osmotic lysisbuffer for "purification" of mitochondria. - (reply: 5)
  92. GST fusion protein purification - (reply: 2)
  93. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
  94. pcr purification - (reply: 1)
  95. pcr purification doubt - (reply: 4)
  96. Mouse IgG1 purification - Protein G - (reply: 1)
  97. How to purify antioxidants enzymes from plant tissue. - (reply: 1)
  98. pcr purification - (reply: 1)
  99. Use PCR purification kit to purify restriction digestion - (reply: 2)
  100. Help with His-Tag protein purification - (reply: 2)
  101. protien purification using flag affinity - (reply: 4)
  102. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
  103. MW of PEG for Purification of DNA - (reply: 2)
  104. HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
  105. Reversed-Phase chromatography for protein purification - (reply: 3)
  106. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  107. problem with purification of gst-tagged protein - (reply: 2)
  108. Saliva and GCF DNA purification - (reply: 1)
  109. DNA isolation/purification troubleshooting. - (reply: 5)
  110. How to purify RNA from a handful number of cells? - (reply: 5)
  111. RNA purification from urine samples - (reply: 2)
  112. Purifying recombinant protein from pellet? - (reply: 2)
  113. Purifying RNA from Solution with RNEasy? - (reply: 2)
  114. Columns for ssDNA oligo purification - (reply: 2)
  115. low concentration of purified PCR product - (reply: 2)
  116. 5' RACE cDNA Concentration After SNAP Purification? - (reply: 3)
  117. what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
  118. Extra bands after protein expression/purification - (reply: 4)
  119. PCRed on restriction sites -- how do I purify it? - (reply: 8)
  120. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  121. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  122. Troubleshooting for DNA purification - (reply: 1)
  123. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  124. Insoluble Protein purification - (reply: 1)
  125. How to purify unstable protein? - (reply: 10)
  126. Purification of oligos for EMSA - (reply: 1)
  127. Hisx18 purification - (reply: 4)
  128. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  129. getting rid of imidazole in purified protein? - (reply: 2)
  130. BN-PAGE of purified membrane associated protein - (reply: 5)
  131. Anion exchange purification - (reply: 3)
  132. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  133. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  134. LDH purification - (reply: 1)
  135. NO yield after purification using Qiagen kits - Please help! - (reply: 1)
  136. A problem with IgG purification by Protein G chromatography - (reply: 4)
  137. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  138. Help: I am loosing the DNA during PCR purification - (reply: 7)
  139. FACS Sorting and Protein Purification - (reply: 5)
  140. His-Taq purification - (reply: 4)
  141. Protein purification buffer - (reply: 3)
  142. Protein purification in native conditions - (reply: 1)
  143. Butyl-agarose/sepharose purification - (reply: 1)
  144. methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
  145. type of IgG in my sample?? - proteinA purified sample (reply: 1)
  146. Help with small protein with his-tag purification - (reply: 7)
  147. Purification of DNA after MeDIP and WGA - (reply: 1)
  148. Protein purification from HEK 293 cells - (reply: 1)
  149. ~200kb Plasmid DNA purification is not working - (reply: 1)
  150. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  151. protein purification - (reply: 1)
  152. plant protein purification opinion - (reply: 3)
  153. Unable to Purify protein! - Protein purification (reply: 2)
  154. Problem with Pierce GST spin purification column - (reply: 6)
  155. HIS-tagged protein purification - (reply: 12)
  156. His-tagged protein purification problem - (reply: 2)
  157. Inclusion body purification - (reply: 1)
  158. Purification of recombinant proteins from insect cells - (reply: 2)
  159. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
  160. No DNA after PCR purification with QiaQuick? - (reply: 4)
  161. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  162. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
  163. Multiple bands from purified PCR product - (reply: 3)
  164. Troubleshooting Protein Purification using FPLC - (reply: 1)
  165. protein yield is very low after purification - protein purification (reply: 9)
  166. His tag protein purification - (reply: 7)
  167. Help with GST protein purification - (reply: 4)
  168. Purification of His tagged protein - (reply: 2)
  169. Protein purification. How to make function of recombinant protein? - (reply: 2)
  170. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
  171. purification of PCR product for cloning in a vector - (reply: 3)
  172. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
  173. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  174. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
  175. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  176. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  177. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  178. Decrease in concentration after protein purification - (reply: 3)
  179. inactive enzyme from purification - (reply: 1)
  180. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
  181. His-tagged protein purification - (reply: 3)
  182. protein purification - (reply: 3)
  183. Purification od insoluble protein (TIMP3) - (reply: 2)
  184. white residue after DNA purification - (reply: 3)
  185. Problems with qiaquick gel purification kit - cloning (reply: 10)
  186. problem in protein purification - (reply: 5)
  187. protein purification - (reply: 5)
  188. Help Me With Polyclonal Production - Rabbit Serum Purification (reply: 8)
  189. Problem with protein purification By FPLC - (reply: 4)
  190. uncut vector contamination in gel purified band - (reply: 2)
  191. Purify Chlorella Sample - (reply: 3)
  192. Purified lambda phages - (reply: 1)
  193. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  194. DNA purification from blood - (reply: 6)
  195. Preparation of Purified Inclusion Bodies - (reply: 2)
  196. salt for DNA precipitation - DNA purification (reply: 3)
  197. DNA quantification after gel purification - (reply: 6)
  198. Protein Purification by precipitation - (reply: 2)
  199. Problem with MBP and GST tag purification - (reply: 1)
  200. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
  201. Protein TAG for affinity purification - (reply: 1)
  202. M2 Flag purification - (reply: 2)
  203. Recombinant protein aggregation after purification - (reply: 2)
  204. best and simple method to purify protein - (reply: 38)
  205. strange problem with His purification - (reply: 1)
  206. ChIP purification method - (reply: 7)
  207. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
  208. antibody purification - ammonium sulfate precipitation - (reply: 7)
  209. Problem with Histag purification - (reply: 1)
  210. How to use promaga invitro translated pr. for Pierce EMSA? - translated Pr. purify needed? (reply: 3)
  211. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
  212. IMPACT system purification problem - (reply: 1)
  213. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  214. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
  215. Purification of Protein Extract for ELISA - (reply: 1)
  216. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  217. Help...purify and concentrate protein for IEF - (reply: 1)
  218. NI-NTA purification - (reply: 2)
  219. What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
  220. DNA purification - DNA purification dissolved in water (reply: 3)
  221. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  222. Purification problems his-tag - I have tried everything (reply: 3)
  223. weak purified antibody - (reply: 4)
  224. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
  225. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  226. His Tag Protein Purification - (reply: 6)
  227. Inclusion Body purification - (reply: 2)
  228. membrane protein purification - (reply: 6)
  229. Inseparable insert in gel purification of the vector - (reply: 3)
  230. Contamination in GST fusion protein purification - (reply: 2)
  231. Purification of scretory protein from bacteria - (reply: 2)
  232. Ni-NTA his tag protein purification - (reply: 1)
  233. Tips for antibody purification from crude serum? - (reply: 3)
  234. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
  235. Problems with affinity purification with M2 beads - (reply: 2)
  236. plasmid purification kit for transfection - (reply: 2)
  237. best plasmid purification kit for transfection - (reply: 1)
  238. Purify ChIP DNA using Qiagen spin filter - (reply: 8)
  239. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  240. problem in the GST fusion protein purification - (reply: 4)
  241. Troubleshooting GST purification - (reply: 4)
  242. Storing Purified Proteins - Seeking a Preservative (reply: 6)
  243. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  244. purification of a large amount of DNA - (reply: 3)
  245. Further DNA purification steps needed - Post DNAeasy Kit Purification (reply: 4)
  246. affinity purification and proteomics - (reply: 1)
  247. protein is degraded after purification - (reply: 2)
  248. problem in pcr and purification - (reply: 3)
  249. exosome purification - (reply: 2)
  250. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  251. PCR product purification - (reply: 3)
  252. Protein purification from Insect media - (reply: 1)
  253. Purifying a strange native protein - (reply: 6)
  254. Purification of virus particles - (reply: 2)
  255. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  256. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
  257. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  258. Couple questions about GST purification - (reply: 16)
  259. Rabbit IgG purification - Rabbit IgG purification (reply: 5)
  260. Trypsin Expression and Purification - (reply: 3)
  261. Purifying and cloning 69nt product - (reply: 5)
  262. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  263. dnase treatment before his-tag purification - (reply: 3)
  264. vortexing after phenol-chloroform-IMA purification - (reply: 3)
  265. His tag protein purification - (reply: 3)
  266. GST-fusion protein purification - How to purify it? (reply: 9)
  267. Gel Purification problem..Help~! - (reply: 7)
  268. mAb purification but precipitated - (reply: 13)
  269. Ab purification - (reply: 1)
  270. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  271. Problems in His-tag protein purification with TALON - (reply: 1)
  272. Endogenous protein purification - (reply: 2)
  273. purifying MMP9 - tagging sepharose beads with gelatin (reply: 1)
  274. Ask for help about the RNA purification protocol by phenol/chlorform methods - Ask for help about the RNA purification protocol by phenol/chlorform m (reply: 2)
  275. PCR and gel purification problem - did I screw this up (reply: 4)
  276. Purifying hydrogen bonded peripheral protein - (reply: 3)
  277. GST-tag protein purification - GST-tag protein purification (reply: 4)
  278. Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
  279. How to purify a transmembrane protein from others proteins? - (reply: 1)
  280. SDS Page Help - using purified protein (reply: 3)
  281. GST fusion protein purification - (reply: 1)
  282. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  283. B-per GST fusion protein purification kit - (reply: 2)
  284. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  285. Purifying protein with Neutravidin binding peptide tag - Protein purification (reply: 6)
  286. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
  287. how to concentrate/purify virus without ultracentrifugation? - (reply: 5)
  288. a doubt about PCR gel purification - (reply: 4)
  289. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
  290. Problem with DNA purification from agarose gel - (reply: 3)
  291. DNA purification - long fragments wanted - (reply: 1)
  292. purification of a basic protein - problem in elution (reply: 4)
  293. stabilization of purified protein solutions - (reply: 2)
  294. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  295. silica purification - problem with double bands after silica purification (reply: 1)
  296. Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
  297. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
  298. Gel purification of vector DNA - low yield - (reply: 26)
  299. gel purification or ligation problem - (reply: 3)
  300. Help with Purifying DNA Hairpin - (reply: 2)
  301. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  302. protein purification - (reply: 5)