Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Purification Related Discussions
  1. Protein purification questions - (reply: 3)
  2. Why is my His-SUMO tag being cleaved during purification? - (reply: 1)
  3. low copy number plasmid purification kit - (reply: 4)
  4. Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
  5. Virus vs protein purification - (reply: 3)
  6. BAC DNA purification - (reply: 4)
  7. How to make antibody based affinity column for protein purification - (reply: 4)
  8. protein purification from agarose beads using immunoprecipation kit - (reply: 2)
  9. Fab antibody fragment purification - (reply: 2)
  10. Purification of linearized AT-rich DNA for transfection - (reply: 4)
  11. Plasmid purification for Gibson Assembly - (reply: 2)
  12. HSP70 contamination after Nickel purification - (reply: 3)
  13. Doubt on HisTag protein purification miniprep - (reply: 3)
  14. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
  15. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
  16. Affinity purification glycine elution chemistry - (reply: 3)
  17. storage conditions for purified protein - (reply: 2)
  18. large scale protein purification - (reply: 6)
  19. Gel Purification: When is it safe to pause? - (reply: 2)
  20. after protein purification - (reply: 4)
  21. Who can advise me a kit that would allow me to purify amplicons from quantitativ - (reply: 3)
  22. Cloning purified PCR product into cells - (reply: 5)
  23. antibody/DNAconjugation and purification - (reply: 1)
  24. Affinity Purification of 5′ Capped mRNA by Using High-Affinity GST-4E - (reply: 1)
  25. Question about MBP purification - (reply: 2)
  26. RNA purification with TRI_ low yield - (reply: 1)
  27. Why Iím not getting any band after column purification? - (reply: 1)
  28. Proteinase extraction and purification - (reply: 1)
  29. Protocols to identify or purify a bacterial beta-hemolysin from blood agar? - (reply: 4)
  30. Purification of metallothioneins? - (reply: 1)
  31. Low 260/230 ratios from DNA purification question - (reply: 1)
  32. ChIP: Inconsistent reverse crosslinking and DNA purification result - (reply: 2)
  33. Please help me :Protein expression and purification - (reply: 1)
  34. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
  35. Bacterial DNA extraction/purification from animal tissue - (reply: 1)
  36. DNA virus purification from agarose failed! - (reply: 7)
  37. Any real difference between DNA purification kits? - (reply: 9)
  38. how much purify protein nedded for IP - (reply: 9)
  39. regarding protein purification using 8m urea and sds page - (reply: 2)
  40. Protein Purification from mammalian cells - (reply: 1)
  41. Which method for DNA purification b4 cloning - (reply: 4)
  42. Purification for TOPO - (reply: 1)
  43. Purified DNA fragments are bigger than unpurified - (reply: 2)
  44. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
  45. his-tag purification - (reply: 9)
  46. IgG purification from hybridomas - (reply: 2)
  47. DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
  48. faint dna band after pcr purification - (reply: 1)
  49. transformation and protein purification calculation help; very basic - (reply: 5)
  50. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  51. Plasmid digestion and gel purification using HindIII - (reply: 3)
  52. Do protein purification under partially denatured condition - (reply: 1)
  53. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
  54. Gel purification (DNA) - (reply: 1)
  55. Viral contamination in DNA purification columns - (reply: 1)
  56. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  57. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  58. Protein Purification-catastrophe while purifying? - (reply: 2)
  59. What is the membrane made of in DNA purification kits - (reply: 3)
  60. Protein purification for adherent mammalian cells - (reply: 2)
  61. pGEX-2t purification problem - (reply: 3)
  62. Problem with strep tagged protein purification - (reply: 5)
  63. protein purification in acanthamoeba lysate - (reply: 3)
  64. Polyclonal Ab purification - (reply: 1)
  65. purify protein from native gel - (reply: 1)
  66. purifying proteins with repeats - (reply: 3)
  67. PCR RFLP is PCR product purification necessary? - (reply: 3)
  68. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  69. Protein purification from greenish supernatant of Pichia pastoris - (reply: 10)
  70. Streptavidin purification in the lab - (reply: 4)
  71. adding Tween80 to bacterial culture for protein purification ?! - (reply: 3)
  72. Phenol Chloroform Isoamyl Alcohol (25:24:21) DNA purification - (reply: 3)
  73. No DNA after PCR product purification - (reply: 9)
  74. removal of ethanol in PCR product purification - (reply: 5)
  75. Trouble with purified recombinant protein bands - (reply: 3)
  76. Protein activation after purification - (reply: 4)
  77. Contaminants in protein purification.....help! - (reply: 3)
  78. Protein expression and purification - Please help - (reply: 2)
  79. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  80. tag for protein purification - (reply: 7)
  81. Low yield PCR product after gel purification - (reply: 8)
  82. Trouble in GST- fusion protein purification - (reply: 1)
  83. low concentration protein purification - (reply: 1)
  84. Calculating amount of Beads for purification of Ig - (reply: 3)
  85. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  86. PCR product purification - (reply: 4)
  87. EMSA: cell extract vs purified protein - (reply: 2)
  88. Protein purification method to isolate elicitor with biological activity - (reply: 1)
  89. purification and binding of protein in denaturing conditions - (reply: 2)
  90. problem with Enzyme purification - (reply: 4)
  91. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
  92. Protein Purification from Polyacrylamide Gels - (reply: 3)
  93. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  94. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  95. Purifying a "protein" - (reply: 2)
  96. Help! Purifying native protein-2 years and counting... - (reply: 4)
  97. Large scale Viral RNA purification Kit - (reply: 3)
  98. phage lysate purification - (reply: 2)
  99. the virus particles purification methods - (reply: 7)
  100. Silanized equipment when purifying tRNA? - (reply: 1)
  101. Plasmid Purification and Digestion Problem - (reply: 2)
  102. Purification of FC tagged proteins - (reply: 3)
  103. Hypo-osmotic lysisbuffer for "purification" of mitochondria. - (reply: 5)
  104. GST fusion protein purification - (reply: 2)
  105. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
  106. pcr purification - (reply: 1)
  107. pcr purification doubt - (reply: 4)
  108. Mouse IgG1 purification - Protein G - (reply: 1)
  109. How to purify antioxidants enzymes from plant tissue. - (reply: 1)
  110. pcr purification - (reply: 1)
  111. Use PCR purification kit to purify restriction digestion - (reply: 2)
  112. Help with His-Tag protein purification - (reply: 2)
  113. protien purification using flag affinity - (reply: 4)
  114. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
  115. MW of PEG for Purification of DNA - (reply: 2)
  116. HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
  117. Reversed-Phase chromatography for protein purification - (reply: 3)
  118. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  119. problem with purification of gst-tagged protein - (reply: 2)
  120. Saliva and GCF DNA purification - (reply: 1)
  121. DNA isolation/purification troubleshooting. - (reply: 5)
  122. How to purify RNA from a handful number of cells? - (reply: 5)
  123. RNA purification from urine samples - (reply: 2)
  124. Purifying recombinant protein from pellet? - (reply: 2)
  125. Purifying RNA from Solution with RNEasy? - (reply: 2)
  126. Columns for ssDNA oligo purification - (reply: 2)
  127. low concentration of purified PCR product - (reply: 2)
  128. 5' RACE cDNA Concentration After SNAP Purification? - (reply: 3)
  129. what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
  130. Extra bands after protein expression/purification - (reply: 4)
  131. PCRed on restriction sites -- how do I purify it? - (reply: 8)
  132. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  133. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  134. Troubleshooting for DNA purification - (reply: 1)
  135. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  136. Insoluble Protein purification - (reply: 1)
  137. How to purify unstable protein? - (reply: 10)
  138. Purification of oligos for EMSA - (reply: 1)
  139. Hisx18 purification - (reply: 4)
  140. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  141. getting rid of imidazole in purified protein? - (reply: 2)
  142. BN-PAGE of purified membrane associated protein - (reply: 5)
  143. Anion exchange purification - (reply: 3)
  144. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  145. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  146. LDH purification - (reply: 1)
  147. NO yield after purification using Qiagen kits - Please help! - (reply: 1)
  148. A problem with IgG purification by Protein G chromatography - (reply: 4)
  149. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  150. Help: I am loosing the DNA during PCR purification - (reply: 7)
  151. FACS Sorting and Protein Purification - (reply: 5)
  152. His-Taq purification - (reply: 4)
  153. Protein purification buffer - (reply: 3)
  154. Protein purification in native conditions - (reply: 1)
  155. Butyl-agarose/sepharose purification - (reply: 1)
  156. methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
  157. type of IgG in my sample?? - proteinA purified sample (reply: 1)
  158. Help with small protein with his-tag purification - (reply: 7)
  159. Purification of DNA after MeDIP and WGA - (reply: 1)
  160. Protein purification from HEK 293 cells - (reply: 1)
  161. ~200kb Plasmid DNA purification is not working - (reply: 1)
  162. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  163. protein purification - (reply: 1)
  164. plant protein purification opinion - (reply: 3)
  165. Unable to Purify protein! - Protein purification (reply: 2)
  166. Problem with Pierce GST spin purification column - (reply: 6)
  167. HIS-tagged protein purification - (reply: 12)
  168. His-tagged protein purification problem - (reply: 2)
  169. Inclusion body purification - (reply: 1)
  170. Purification of recombinant proteins from insect cells - (reply: 2)
  171. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
  172. No DNA after PCR purification with QiaQuick? - (reply: 4)
  173. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  174. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
  175. Multiple bands from purified PCR product - (reply: 3)
  176. Troubleshooting Protein Purification using FPLC - (reply: 1)
  177. protein yield is very low after purification - protein purification (reply: 9)
  178. His tag protein purification - (reply: 7)
  179. Help with GST protein purification - (reply: 4)
  180. Purification of His tagged protein - (reply: 2)
  181. Protein purification. How to make function of recombinant protein? - (reply: 2)
  182. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
  183. purification of PCR product for cloning in a vector - (reply: 3)
  184. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
  185. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  186. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
  187. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  188. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  189. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  190. Decrease in concentration after protein purification - (reply: 3)
  191. inactive enzyme from purification - (reply: 1)
  192. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
  193. His-tagged protein purification - (reply: 3)
  194. protein purification - (reply: 3)
  195. Purification od insoluble protein (TIMP3) - (reply: 2)
  196. white residue after DNA purification - (reply: 3)
  197. Problems with qiaquick gel purification kit - cloning (reply: 10)
  198. problem in protein purification - (reply: 5)
  199. protein purification - (reply: 5)
  200. Help Me With Polyclonal Production - Rabbit Serum Purification (reply: 8)
  201. Problem with protein purification By FPLC - (reply: 4)
  202. uncut vector contamination in gel purified band - (reply: 2)
  203. Purify Chlorella Sample - (reply: 3)
  204. Purified lambda phages - (reply: 1)
  205. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  206. DNA purification from blood - (reply: 6)
  207. Preparation of Purified Inclusion Bodies - (reply: 2)
  208. salt for DNA precipitation - DNA purification (reply: 3)
  209. DNA quantification after gel purification - (reply: 6)
  210. Protein Purification by precipitation - (reply: 2)
  211. Problem with MBP and GST tag purification - (reply: 1)
  212. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
  213. Protein TAG for affinity purification - (reply: 1)
  214. M2 Flag purification - (reply: 2)
  215. Recombinant protein aggregation after purification - (reply: 2)
  216. best and simple method to purify protein - (reply: 38)
  217. strange problem with His purification - (reply: 1)
  218. ChIP purification method - (reply: 7)
  219. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
  220. antibody purification - ammonium sulfate precipitation - (reply: 7)
  221. Problem with Histag purification - (reply: 1)
  222. How to use promaga invitro translated pr. for Pierce EMSA? - translated Pr. purify needed? (reply: 3)
  223. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
  224. IMPACT system purification problem - (reply: 1)
  225. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  226. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
  227. Purification of Protein Extract for ELISA - (reply: 1)
  228. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  229. Help...purify and concentrate protein for IEF - (reply: 1)
  230. NI-NTA purification - (reply: 2)
  231. What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
  232. DNA purification - DNA purification dissolved in water (reply: 3)
  233. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  234. Purification problems his-tag - I have tried everything (reply: 3)
  235. weak purified antibody - (reply: 4)
  236. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
  237. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  238. His Tag Protein Purification - (reply: 6)
  239. Inclusion Body purification - (reply: 2)
  240. membrane protein purification - (reply: 6)
  241. Inseparable insert in gel purification of the vector - (reply: 3)
  242. Contamination in GST fusion protein purification - (reply: 2)
  243. Purification of scretory protein from bacteria - (reply: 2)
  244. Ni-NTA his tag protein purification - (reply: 1)
  245. Tips for antibody purification from crude serum? - (reply: 3)
  246. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
  247. Problems with affinity purification with M2 beads - (reply: 2)
  248. plasmid purification kit for transfection - (reply: 2)
  249. best plasmid purification kit for transfection - (reply: 1)
  250. Purify ChIP DNA using Qiagen spin filter - (reply: 8)
  251. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  252. problem in the GST fusion protein purification - (reply: 4)
  253. Troubleshooting GST purification - (reply: 4)
  254. Storing Purified Proteins - Seeking a Preservative (reply: 6)
  255. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  256. purification of a large amount of DNA - (reply: 3)
  257. Further DNA purification steps needed - Post DNAeasy Kit Purification (reply: 4)
  258. affinity purification and proteomics - (reply: 1)
  259. protein is degraded after purification - (reply: 2)
  260. problem in pcr and purification - (reply: 3)
  261. exosome purification - (reply: 2)
  262. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  263. PCR product purification - (reply: 3)
  264. Protein purification from Insect media - (reply: 1)
  265. Purifying a strange native protein - (reply: 6)
  266. Purification of virus particles - (reply: 2)
  267. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  268. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
  269. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  270. Couple questions about GST purification - (reply: 16)
  271. Rabbit IgG purification - Rabbit IgG purification (reply: 5)
  272. Trypsin Expression and Purification - (reply: 3)
  273. Purifying and cloning 69nt product - (reply: 5)
  274. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  275. dnase treatment before his-tag purification - (reply: 3)
  276. vortexing after phenol-chloroform-IMA purification - (reply: 3)
  277. His tag protein purification - (reply: 3)
  278. GST-fusion protein purification - How to purify it? (reply: 9)
  279. Gel Purification problem..Help~! - (reply: 7)
  280. mAb purification but precipitated - (reply: 13)
  281. Ab purification - (reply: 1)
  282. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  283. Problems in His-tag protein purification with TALON - (reply: 1)
  284. Endogenous protein purification - (reply: 2)
  285. purifying MMP9 - tagging sepharose beads with gelatin (reply: 1)
  286. Ask for help about the RNA purification protocol by phenol/chlorform methods - Ask for help about the RNA purification protocol by phenol/chlorform m (reply: 2)
  287. PCR and gel purification problem - did I screw this up (reply: 4)
  288. Purifying hydrogen bonded peripheral protein - (reply: 3)
  289. GST-tag protein purification - GST-tag protein purification (reply: 4)
  290. Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
  291. How to purify a transmembrane protein from others proteins? - (reply: 1)
  292. SDS Page Help - using purified protein (reply: 3)
  293. GST fusion protein purification - (reply: 1)
  294. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  295. B-per GST fusion protein purification kit - (reply: 2)
  296. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  297. Purifying protein with Neutravidin binding peptide tag - Protein purification (reply: 6)
  298. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
  299. how to concentrate/purify virus without ultracentrifugation? - (reply: 5)
  300. a doubt about PCR gel purification - (reply: 4)
  301. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
  302. Problem with DNA purification from agarose gel - (reply: 3)
  303. DNA purification - long fragments wanted - (reply: 1)
  304. purification of a basic protein - problem in elution (reply: 4)
  305. stabilization of purified protein solutions - (reply: 2)
  306. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  307. silica purification - problem with double bands after silica purification (reply: 1)
  308. Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
  309. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
  310. Gel purification of vector DNA - low yield - (reply: 26)
  311. gel purification or ligation problem - (reply: 3)
  312. Help with Purifying DNA Hairpin - (reply: 2)
  313. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  314. protein purification - (reply: 5)