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Purification Related Discussions
  1. Gel Purification: When is it safe to pause? - (reply: 2)
  2. after protein purification - (reply: 4)
  3. Who can advise me a kit that would allow me to purify amplicons from quantitativ - (reply: 3)
  4. Cloning purified PCR product into cells - (reply: 5)
  5. antibody/DNAconjugation and purification - (reply: 1)
  6. Affinity Purification of 5′ Capped mRNA by Using High-Affinity GST-4E - (reply: 1)
  7. Question about MBP purification - (reply: 2)
  8. RNA purification with TRI_ low yield - (reply: 1)
  9. Why Iím not getting any band after column purification? - (reply: 1)
  10. Proteinase extraction and purification - (reply: 1)
  11. Protocols to identify or purify a bacterial beta-hemolysin from blood agar? - (reply: 4)
  12. Purification of metallothioneins? - (reply: 1)
  13. Low 260/230 ratios from DNA purification question - (reply: 1)
  14. ChIP: Inconsistent reverse crosslinking and DNA purification result - (reply: 2)
  15. Please help me :Protein expression and purification - (reply: 1)
  16. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
  17. Bacterial DNA extraction/purification from animal tissue - (reply: 1)
  18. DNA virus purification from agarose failed! - (reply: 7)
  19. Any real difference between DNA purification kits? - (reply: 9)
  20. how much purify protein nedded for IP - (reply: 9)
  21. regarding protein purification using 8m urea and sds page - (reply: 2)
  22. Protein Purification from mammalian cells - (reply: 1)
  23. Which method for DNA purification b4 cloning - (reply: 4)
  24. Purification for TOPO - (reply: 1)
  25. Purified DNA fragments are bigger than unpurified - (reply: 2)
  26. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
  27. his-tag purification - (reply: 9)
  28. IgG purification from hybridomas - (reply: 2)
  29. DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
  30. faint dna band after pcr purification - (reply: 1)
  31. transformation and protein purification calculation help; very basic - (reply: 5)
  32. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  33. Plasmid digestion and gel purification using HindIII - (reply: 3)
  34. Do protein purification under partially denatured condition - (reply: 1)
  35. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
  36. Gel purification (DNA) - (reply: 1)
  37. Viral contamination in DNA purification columns - (reply: 1)
  38. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  39. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  40. Protein Purification-catastrophe while purifying? - (reply: 2)
  41. What is the membrane made of in DNA purification kits - (reply: 3)
  42. Protein purification for adherent mammalian cells - (reply: 2)
  43. pGEX-2t purification problem - (reply: 3)
  44. Problem with strep tagged protein purification - (reply: 5)
  45. protein purification in acanthamoeba lysate - (reply: 3)
  46. Polyclonal Ab purification - (reply: 1)
  47. purify protein from native gel - (reply: 1)
  48. purifying proteins with repeats - (reply: 3)
  49. PCR RFLP is PCR product purification necessary? - (reply: 3)
  50. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  51. Protein purification from greenish supernatant of Pichia pastoris - (reply: 10)
  52. Streptavidin purification in the lab - (reply: 4)
  53. adding Tween80 to bacterial culture for protein purification ?! - (reply: 3)
  54. Phenol Chloroform Isoamyl Alcohol (25:24:21) DNA purification - (reply: 3)
  55. No DNA after PCR product purification - (reply: 9)
  56. removal of ethanol in PCR product purification - (reply: 5)
  57. Trouble with purified recombinant protein bands - (reply: 3)
  58. Protein activation after purification - (reply: 4)
  59. Contaminants in protein purification.....help! - (reply: 3)
  60. Protein expression and purification - Please help - (reply: 2)
  61. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  62. tag for protein purification - (reply: 7)
  63. Low yield PCR product after gel purification - (reply: 8)
  64. Trouble in GST- fusion protein purification - (reply: 1)
  65. low concentration protein purification - (reply: 1)
  66. Calculating amount of Beads for purification of Ig - (reply: 3)
  67. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  68. PCR product purification - (reply: 4)
  69. EMSA: cell extract vs purified protein - (reply: 2)
  70. Protein purification method to isolate elicitor with biological activity - (reply: 1)
  71. purification and binding of protein in denaturing conditions - (reply: 2)
  72. problem with Enzyme purification - (reply: 4)
  73. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
  74. Protein Purification from Polyacrylamide Gels - (reply: 3)
  75. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  76. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  77. Purifying a "protein" - (reply: 2)
  78. Help! Purifying native protein-2 years and counting... - (reply: 4)
  79. Large scale Viral RNA purification Kit - (reply: 3)
  80. phage lysate purification - (reply: 2)
  81. the virus particles purification methods - (reply: 7)
  82. Silanized equipment when purifying tRNA? - (reply: 1)
  83. Plasmid Purification and Digestion Problem - (reply: 2)
  84. Purification of FC tagged proteins - (reply: 3)
  85. Hypo-osmotic lysisbuffer for "purification" of mitochondria. - (reply: 5)
  86. GST fusion protein purification - (reply: 2)
  87. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
  88. pcr purification - (reply: 1)
  89. pcr purification doubt - (reply: 4)
  90. Mouse IgG1 purification - Protein G - (reply: 1)
  91. How to purify antioxidants enzymes from plant tissue. - (reply: 1)
  92. pcr purification - (reply: 1)
  93. Use PCR purification kit to purify restriction digestion - (reply: 2)
  94. Help with His-Tag protein purification - (reply: 2)
  95. protien purification using flag affinity - (reply: 4)
  96. SDS precipitation after adding DNA binding buffer for column purification - (reply: 1)
  97. MW of PEG for Purification of DNA - (reply: 2)
  98. HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
  99. Reversed-Phase chromatography for protein purification - (reply: 3)
  100. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  101. problem with purification of gst-tagged protein - (reply: 2)
  102. Saliva and GCF DNA purification - (reply: 1)
  103. DNA isolation/purification troubleshooting. - (reply: 5)
  104. How to purify RNA from a handful number of cells? - (reply: 5)
  105. RNA purification from urine samples - (reply: 2)
  106. Purifying recombinant protein from pellet? - (reply: 2)
  107. Purifying RNA from Solution with RNEasy? - (reply: 2)
  108. Columns for ssDNA oligo purification - (reply: 2)
  109. low concentration of purified PCR product - (reply: 2)
  110. 5' RACE cDNA Concentration After SNAP Purification? - (reply: 3)
  111. what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
  112. Extra bands after protein expression/purification - (reply: 4)
  113. PCRed on restriction sites -- how do I purify it? - (reply: 8)
  114. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  115. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  116. Troubleshooting for DNA purification - (reply: 1)
  117. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  118. Insoluble Protein purification - (reply: 1)
  119. How to purify unstable protein? - (reply: 10)
  120. Purification of oligos for EMSA - (reply: 1)
  121. Hisx18 purification - (reply: 4)
  122. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  123. getting rid of imidazole in purified protein? - (reply: 2)
  124. BN-PAGE of purified membrane associated protein - (reply: 5)
  125. Anion exchange purification - (reply: 3)
  126. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  127. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  128. LDH purification - (reply: 1)
  129. NO yield after purification using Qiagen kits - Please help! - (reply: 1)
  130. A problem with IgG purification by Protein G chromatography - (reply: 4)
  131. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  132. Help: I am loosing the DNA during PCR purification - (reply: 7)
  133. FACS Sorting and Protein Purification - (reply: 5)
  134. His-Taq purification - (reply: 4)
  135. Protein purification buffer - (reply: 3)
  136. Protein purification in native conditions - (reply: 1)
  137. Butyl-agarose/sepharose purification - (reply: 1)
  138. methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
  139. type of IgG in my sample?? - proteinA purified sample (reply: 1)
  140. Help with small protein with his-tag purification - (reply: 7)
  141. Purification of DNA after MeDIP and WGA - (reply: 1)
  142. Protein purification from HEK 293 cells - (reply: 1)
  143. ~200kb Plasmid DNA purification is not working - (reply: 1)
  144. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  145. protein purification - (reply: 1)
  146. plant protein purification opinion - (reply: 3)
  147. Unable to Purify protein! - Protein purification (reply: 2)
  148. Problem with Pierce GST spin purification column - (reply: 6)
  149. HIS-tagged protein purification - (reply: 12)
  150. His-tagged protein purification problem - (reply: 2)
  151. Inclusion body purification - (reply: 1)
  152. Purification of recombinant proteins from insect cells - (reply: 2)
  153. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
  154. No DNA after PCR purification with QiaQuick? - (reply: 4)
  155. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  156. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
  157. Multiple bands from purified PCR product - (reply: 3)
  158. Troubleshooting Protein Purification using FPLC - (reply: 1)
  159. protein yield is very low after purification - protein purification (reply: 9)
  160. His tag protein purification - (reply: 7)
  161. Help with GST protein purification - (reply: 4)
  162. Purification of His tagged protein - (reply: 2)
  163. Protein purification. How to make function of recombinant protein? - (reply: 2)
  164. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
  165. purification of PCR product for cloning in a vector - (reply: 3)
  166. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
  167. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  168. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
  169. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  170. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  171. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  172. Decrease in concentration after protein purification - (reply: 3)
  173. inactive enzyme from purification - (reply: 1)
  174. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
  175. His-tagged protein purification - (reply: 3)
  176. protein purification - (reply: 3)
  177. Purification od insoluble protein (TIMP3) - (reply: 2)
  178. white residue after DNA purification - (reply: 3)
  179. Problems with qiaquick gel purification kit - cloning (reply: 10)
  180. problem in protein purification - (reply: 5)
  181. protein purification - (reply: 5)
  182. Help Me With Polyclonal Production - Rabbit Serum Purification (reply: 8)
  183. Problem with protein purification By FPLC - (reply: 4)
  184. uncut vector contamination in gel purified band - (reply: 2)
  185. Purify Chlorella Sample - (reply: 3)
  186. Purified lambda phages - (reply: 1)
  187. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  188. DNA purification from blood - (reply: 6)
  189. Preparation of Purified Inclusion Bodies - (reply: 2)
  190. salt for DNA precipitation - DNA purification (reply: 3)
  191. DNA quantification after gel purification - (reply: 6)
  192. Protein Purification by precipitation - (reply: 2)
  193. Problem with MBP and GST tag purification - (reply: 1)
  194. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
  195. Protein TAG for affinity purification - (reply: 1)
  196. M2 Flag purification - (reply: 2)
  197. Recombinant protein aggregation after purification - (reply: 2)
  198. best and simple method to purify protein - (reply: 38)
  199. strange problem with His purification - (reply: 1)
  200. ChIP purification method - (reply: 7)
  201. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
  202. antibody purification - ammonium sulfate precipitation - (reply: 7)
  203. Problem with Histag purification - (reply: 1)
  204. How to use promaga invitro translated pr. for Pierce EMSA? - translated Pr. purify needed? (reply: 3)
  205. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
  206. IMPACT system purification problem - (reply: 1)
  207. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  208. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
  209. Purification of Protein Extract for ELISA - (reply: 1)
  210. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  211. Help...purify and concentrate protein for IEF - (reply: 1)
  212. NI-NTA purification - (reply: 2)
  213. What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
  214. DNA purification - DNA purification dissolved in water (reply: 3)
  215. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  216. Purification problems his-tag - I have tried everything (reply: 3)
  217. weak purified antibody - (reply: 4)
  218. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
  219. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  220. His Tag Protein Purification - (reply: 6)
  221. Inclusion Body purification - (reply: 2)
  222. membrane protein purification - (reply: 6)
  223. Inseparable insert in gel purification of the vector - (reply: 3)
  224. Contamination in GST fusion protein purification - (reply: 2)
  225. Purification of scretory protein from bacteria - (reply: 2)
  226. Ni-NTA his tag protein purification - (reply: 1)
  227. Tips for antibody purification from crude serum? - (reply: 3)
  228. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
  229. Problems with affinity purification with M2 beads - (reply: 2)
  230. plasmid purification kit for transfection - (reply: 2)
  231. best plasmid purification kit for transfection - (reply: 1)
  232. Purify ChIP DNA using Qiagen spin filter - (reply: 8)
  233. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  234. problem in the GST fusion protein purification - (reply: 4)
  235. Troubleshooting GST purification - (reply: 4)
  236. Storing Purified Proteins - Seeking a Preservative (reply: 6)
  237. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  238. purification of a large amount of DNA - (reply: 3)
  239. Further DNA purification steps needed - Post DNAeasy Kit Purification (reply: 4)
  240. affinity purification and proteomics - (reply: 1)
  241. protein is degraded after purification - (reply: 2)
  242. problem in pcr and purification - (reply: 3)
  243. exosome purification - (reply: 2)
  244. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  245. PCR product purification - (reply: 3)
  246. Protein purification from Insect media - (reply: 1)
  247. Purifying a strange native protein - (reply: 6)
  248. Purification of virus particles - (reply: 2)
  249. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  250. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
  251. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  252. Couple questions about GST purification - (reply: 16)
  253. Rabbit IgG purification - Rabbit IgG purification (reply: 5)
  254. Trypsin Expression and Purification - (reply: 3)
  255. Purifying and cloning 69nt product - (reply: 5)
  256. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  257. dnase treatment before his-tag purification - (reply: 3)
  258. vortexing after phenol-chloroform-IMA purification - (reply: 3)
  259. His tag protein purification - (reply: 3)
  260. GST-fusion protein purification - How to purify it? (reply: 9)
  261. Gel Purification problem..Help~! - (reply: 7)
  262. mAb purification but precipitated - (reply: 13)
  263. Ab purification - (reply: 1)
  264. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  265. Problems in His-tag protein purification with TALON - (reply: 1)
  266. Endogenous protein purification - (reply: 2)
  267. purifying MMP9 - tagging sepharose beads with gelatin (reply: 1)
  268. Ask for help about the RNA purification protocol by phenol/chlorform methods - Ask for help about the RNA purification protocol by phenol/chlorform m (reply: 2)
  269. PCR and gel purification problem - did I screw this up (reply: 4)
  270. Purifying hydrogen bonded peripheral protein - (reply: 3)
  271. GST-tag protein purification - GST-tag protein purification (reply: 4)
  272. Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
  273. How to purify a transmembrane protein from others proteins? - (reply: 1)
  274. SDS Page Help - using purified protein (reply: 3)
  275. GST fusion protein purification - (reply: 1)
  276. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  277. B-per GST fusion protein purification kit - (reply: 2)
  278. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  279. Purifying protein with Neutravidin binding peptide tag - Protein purification (reply: 6)
  280. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
  281. how to concentrate/purify virus without ultracentrifugation? - (reply: 5)
  282. a doubt about PCR gel purification - (reply: 4)
  283. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
  284. Problem with DNA purification from agarose gel - (reply: 3)
  285. DNA purification - long fragments wanted - (reply: 1)
  286. purification of a basic protein - problem in elution (reply: 4)
  287. stabilization of purified protein solutions - (reply: 2)
  288. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  289. silica purification - problem with double bands after silica purification (reply: 1)
  290. Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
  291. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
  292. Gel purification of vector DNA - low yield - (reply: 26)
  293. gel purification or ligation problem - (reply: 3)
  294. Help with Purifying DNA Hairpin - (reply: 2)
  295. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  296. protein purification - (reply: 5)