Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Purification Related Discussions
  1. How to purify RNA from a handful number of cells? - (reply: 5)
  2. RNA purification from urine samples - (reply: 1)
  3. Purifying recombinant protein from pellet? - (reply: 2)
  4. Purifying RNA from Solution with RNEasy? - (reply: 2)
  5. Columns for ssDNA oligo purification - (reply: 2)
  6. low concentration of purified PCR product - (reply: 2)
  7. 5' RACE cDNA Concentration After SNAP Purification? - (reply: 3)
  8. what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
  9. Extra bands after protein expression/purification - (reply: 4)
  10. PCRed on restriction sites -- how do I purify it? - (reply: 8)
  11. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  12. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  13. Troubleshooting for DNA purification - (reply: 1)
  14. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  15. Insoluble Protein purification - (reply: 1)
  16. How to purify unstable protein? - (reply: 10)
  17. Purification of oligos for EMSA - (reply: 1)
  18. Hisx18 purification - (reply: 4)
  19. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  20. getting rid of imidazole in purified protein? - (reply: 2)
  21. BN-PAGE of purified membrane associated protein - (reply: 5)
  22. Anion exchange purification - (reply: 3)
  23. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  24. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  25. LDH purification - (reply: 1)
  26. NO yield after purification using Qiagen kits - Please help! - (reply: 1)
  27. A problem with IgG purification by Protein G chromatography - (reply: 4)
  28. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  29. Help: I am loosing the DNA during PCR purification - (reply: 7)
  30. FACS Sorting and Protein Purification - (reply: 5)
  31. His-Taq purification - (reply: 3)
  32. Protein purification buffer - (reply: 3)
  33. Protein purification in native conditions - (reply: 1)
  34. Butyl-agarose/sepharose purification - (reply: 1)
  35. methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
  36. type of IgG in my sample?? - proteinA purified sample (reply: 1)
  37. Help with small protein with his-tag purification - (reply: 7)
  38. Purification of DNA after MeDIP and WGA - (reply: 1)
  39. Protein purification from HEK 293 cells - (reply: 1)
  40. ~200kb Plasmid DNA purification is not working - (reply: 1)
  41. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  42. protein purification - (reply: 1)
  43. plant protein purification opinion - (reply: 3)
  44. Unable to Purify protein! - Protein purification (reply: 2)
  45. Problem with Pierce GST spin purification column - (reply: 6)
  46. HIS-tagged protein purification - (reply: 12)
  47. His-tagged protein purification problem - (reply: 2)
  48. Inclusion body purification - (reply: 1)
  49. Purification of recombinant proteins from insect cells - (reply: 2)
  50. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
  51. No DNA after PCR purification with QiaQuick? - (reply: 4)
  52. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  53. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
  54. Multiple bands from purified PCR product - (reply: 3)
  55. Troubleshooting Protein Purification using FPLC - (reply: 1)
  56. protein yield is very low after purification - protein purification (reply: 9)
  57. His tag protein purification - (reply: 7)
  58. Help with GST protein purification - (reply: 4)
  59. Purification of His tagged protein - (reply: 2)
  60. Protein purification. How to make function of recombinant protein? - (reply: 2)
  61. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
  62. purification of PCR product for cloning in a vector - (reply: 3)
  63. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
  64. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  65. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
  66. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  67. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  68. Really strange problem with sequencing and plasmid purification! - (reply: 7)
  69. Decrease in concentration after protein purification - (reply: 3)
  70. inactive enzyme from purification - (reply: 1)
  71. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
  72. His-tagged protein purification - (reply: 3)
  73. protein purification - (reply: 3)
  74. Purification od insoluble protein (TIMP3) - (reply: 2)
  75. white residue after DNA purification - (reply: 3)
  76. Problems with qiaquick gel purification kit - cloning (reply: 10)
  77. problem in protein purification - (reply: 5)
  78. protein purification - (reply: 5)
  79. Help Me With Polyclonal Production - Rabbit Serum Purification (reply: 8)
  80. Problem with protein purification By FPLC - (reply: 4)
  81. uncut vector contamination in gel purified band - (reply: 2)
  82. Purify Chlorella Sample - (reply: 3)
  83. Purified lambda phages - (reply: 1)
  84. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  85. DNA purification from blood - (reply: 6)
  86. Preparation of Purified Inclusion Bodies - (reply: 2)
  87. salt for DNA precipitation - DNA purification (reply: 3)
  88. DNA quantification after gel purification - (reply: 6)
  89. Protein Purification by precipitation - (reply: 2)
  90. Problem with MBP and GST tag purification - (reply: 1)
  91. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
  92. Protein TAG for affinity purification - (reply: 1)
  93. M2 Flag purification - (reply: 2)
  94. Recombinant protein aggregation after purification - (reply: 2)
  95. best and simple method to purify protein - (reply: 38)
  96. strange problem with His purification - (reply: 1)
  97. ChIP purification method - (reply: 7)
  98. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
  99. antibody purification - ammonium sulfate precipitation - (reply: 7)
  100. Problem with Histag purification - (reply: 1)
  101. How to use promaga invitro translated pr. for Pierce EMSA? - translated Pr. purify needed? (reply: 3)
  102. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
  103. IMPACT system purification problem - (reply: 1)
  104. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  105. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
  106. Purification of Protein Extract for ELISA - (reply: 1)
  107. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  108. Help...purify and concentrate protein for IEF - (reply: 1)
  109. NI-NTA purification - (reply: 2)
  110. What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
  111. DNA purification - DNA purification dissolved in water (reply: 3)
  112. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  113. Purification problems his-tag - I have tried everything (reply: 3)
  114. weak purified antibody - (reply: 4)
  115. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
  116. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  117. His Tag Protein Purification - (reply: 6)
  118. Inclusion Body purification - (reply: 2)
  119. membrane protein purification - (reply: 6)
  120. Inseparable insert in gel purification of the vector - (reply: 3)
  121. Contamination in GST fusion protein purification - (reply: 2)
  122. Purification of scretory protein from bacteria - (reply: 2)
  123. Ni-NTA his tag protein purification - (reply: 1)
  124. Tips for antibody purification from crude serum? - (reply: 3)
  125. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
  126. Problems with affinity purification with M2 beads - (reply: 2)
  127. plasmid purification kit for transfection - (reply: 2)
  128. best plasmid purification kit for transfection - (reply: 1)
  129. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  130. problem in the GST fusion protein purification - (reply: 4)
  131. Troubleshooting GST purification - (reply: 4)
  132. Storing Purified Proteins - Seeking a Preservative (reply: 7)
  133. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  134. purification of a large amount of DNA - (reply: 3)
  135. Further DNA purification steps needed - Post DNAeasy Kit Purification (reply: 4)
  136. affinity purification and proteomics - (reply: 1)
  137. protein is degraded after purification - (reply: 2)
  138. problem in pcr and purification - (reply: 3)
  139. exosome purification - (reply: 1)
  140. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  141. PCR product purification - (reply: 3)
  142. Protein purification from Insect media - (reply: 1)
  143. Purifying a strange native protein - (reply: 6)
  144. Purification of virus particles - (reply: 2)
  145. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  146. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
  147. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  148. Couple questions about GST purification - (reply: 16)
  149. Rabbit IgG purification - Rabbit IgG purification (reply: 5)
  150. Trypsin Expression and Purification - (reply: 3)
  151. Purifying and cloning 69nt product - (reply: 5)
  152. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  153. dnase treatment before his-tag purification - (reply: 3)
  154. vortexing after phenol-chloroform-IMA purification - (reply: 3)
  155. His tag protein purification - (reply: 3)
  156. GST-fusion protein purification - How to purify it? (reply: 9)
  157. Gel Purification problem..Help~! - (reply: 7)
  158. mAb purification but precipitated - (reply: 13)
  159. Ab purification - (reply: 1)
  160. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  161. Problems in His-tag protein purification with TALON - (reply: 1)
  162. Endogenous protein purification - (reply: 2)
  163. purifying MMP9 - tagging sepharose beads with gelatin (reply: 1)
  164. Ask for help about the RNA purification protocol by phenol/chlorform methods - Ask for help about the RNA purification protocol by phenol/chlorform m (reply: 2)
  165. PCR and gel purification problem - did I screw this up (reply: 4)
  166. Purifying hydrogen bonded peripheral protein - (reply: 3)
  167. GST-tag protein purification - GST-tag protein purification (reply: 4)
  168. Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
  169. How to purify a transmembrane protein from others proteins? - (reply: 1)
  170. SDS Page Help - using purified protein (reply: 3)
  171. GST fusion protein purification - (reply: 1)
  172. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  173. B-per GST fusion protein purification kit - (reply: 2)
  174. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  175. Purifying protein with Neutravidin binding peptide tag - Protein purification (reply: 6)
  176. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
  177. how to concentrate/purify virus without ultracentrifugation? - (reply: 5)
  178. a doubt about PCR gel purification - (reply: 4)
  179. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
  180. Problem with DNA purification from agarose gel - (reply: 3)
  181. DNA purification - long fragments wanted - (reply: 1)
  182. purification of a basic protein - problem in elution (reply: 4)
  183. stabilization of purified protein solutions - (reply: 2)
  184. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  185. silica purification - problem with double bands after silica purification (reply: 1)
  186. Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
  187. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
  188. Gel purification of vector DNA - low yield - (reply: 26)
  189. gel purification or ligation problem - (reply: 3)
  190. Help with Purifying DNA Hairpin - (reply: 2)
  191. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  192. protein purification - (reply: 5)