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vortexing after phenol-chloroform-IMA purification - (Jul/17/2009 )


is it recommended to vortex the sample after phenol-chloroform-isoamylalcohol addition during a plasmid prep? does it shear the DNA?


The protocol says to mix well as far as I can recall. I just shake the tubes hard for 15 sec.


I guess I wouldn't vortex my sample. The longer the DNA, the easier it is to shear it. I guess I will just invert my tubes for about 15 times.


I usually vortex after P:C:I addition and have had no troubles with pDNA or gDNA downstream.