Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
RT-PCR Related Discussions
  1. Length of amplicon for RT real time PCR - (reply: 2)
  2. Amount of cDNA input in RT-PCR - (reply: 1)
  3. testing primers efficiency for rt-PCR - (reply: 1)
  4. RT-PCR low efficiency - (reply: 1)
  5. RT-PCR electrophorasis - (reply: 1)
  6. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
  7. RT-PCR Kit's Validation - (reply: 1)
  8. Rt pcr - (reply: 4)
  9. Experimental design for RT-PCR - (reply: 8)
  10. RT-PCR analysis - (reply: 1)
  11. Absolute quant with TaqMan - Different RT & different plates? - (reply: 1)
  12. how to calculate amount of cDNA using RT-PCR - (reply: 2)
  13. need help setting up reverse transcriptase qPCR - (reply: 3)
  14. How does RT-PCR work? - (reply: 5)
  15. could you help me with my stem loop RT primer? - (reply: 1)
  16. RT-PCR help - (reply: 2)
  17. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
  18. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
  19. Need no RT control for all genes? - (reply: 1)
  20. Question about the RT PCR - (reply: 3)
  21. Internal control for miRNA RT-PCR - (reply: 1)
  22. Can somebody explain to me what "spiking" means in RT-PCR and why do you - (reply: 3)
  23. Reverse transcription 101 question! - (reply: 5)
  24. Quantitative RT-PCR statistics help - (reply: 1)
  25. RNA extraction for RT-PCR - (reply: 3)
  26. RT-PCR primer design - (reply: 7)
  27. skip 65 degrees protocol of Reverse transcription - (reply: 4)
  28. Need help with my real time RT-PCR Plate Set up - (reply: 8)
  29. RNA concentration to start RT reaction - (reply: 1)
  30. Random vs oligo primer in preamplification RT - (reply: 1)
  31. Sensitivity of RT-PCR and qPCR - (reply: 4)
  32. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  33. Detecting miRNA mimics by RT-PCR? - (reply: 4)
  34. A question for Reverse transcription experts - (reply: 5)
  35. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  36. RT-PCR product- no band - (reply: 4)
  37. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
  38. No amplification during RT-PCR - (reply: 4)
  39. Sequencing RT-PCR product - (reply: 3)
  40. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  41. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
  42. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  43. Differentiate between RT-PCR and PCR - (reply: 3)
  44. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
  45. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  46. Strange RT to qPCR results with new kit - (reply: 5)
  47. Plasmid DNA at RT for a few days - (reply: 4)
  48. Controls for RT-PCR reactions - (reply: 2)
  49. What is your preferred Reverse Transcriptase? - (reply: 4)
  50. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  51. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  52. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
  53. Error rate of commecial reverse transcriptase - (reply: 1)
  54. complicated RT-PCR issues - (reply: 4)
  55. RT-PCR vs plate reader for pathogen detection - (reply: 3)
  56. Buffer-composition One-Step RT-PCR - (reply: 4)
  57. RT / cDNA synthesis - (reply: 7)
  58. RT-PCR primer design - (reply: 1)
  59. urgent help plz with RT-PCR - (reply: 3)
  60. Problems with two step RT-PCR - (reply: 2)
  61. RT-PCR contamination in negative control. - (reply: 1)
  62. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
  63. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  64. cDNA synthesis or RT-PCR - (reply: 3)
  65. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
  66. RT-PCR in a single tube - (reply: 3)
  67. Maximum length of cDNA from RT reaction - (reply: 6)
  68. Strange RT-PCR amplification plots - (reply: 9)
  69. troubleshooting RT-PCR: possible nuclease issues? - (reply: 2)
  70. RT-PCR primers and the poly(A) tail - (reply: 2)
  71. RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
  72. real time RT-PCR problems - (reply: 2)
  73. RT-PCR - (reply: 1)
  74. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  75. Rt-PCR primer design - (reply: 7)
  76. staining at 37 degrees or RT - OK or really bad ? - (reply: 3)
  77. ROX reference dye for quantitative RT-PCR - (reply: 1)
  78. Comparative semiquantitative RT-PCR - (reply: 2)
  79. RT-PCR doesn't work with all RNA used - (reply: 1)
  80. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  81. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  82. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  83. RT-PCR calibrator - (reply: 2)
  84. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
  85. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  86. real time rt PCR - bloodyurine - inhibition - (reply: 1)
  87. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
  88. Stability of DNA at RT - (reply: 1)
  89. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
  90. RT-PCR primer design - Primer design and evaluation (reply: 7)
  91. Splice varient quantification in RT real time PCR? - (reply: 1)
  92. Cleaning up RT-PCR product before sequencing - (reply: 1)
  93. RT-PCR cDNA synthesis - (reply: 6)
  94. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
  95. RT-PCR t-test, and ANOVA - (reply: 1)
  96. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
  97. RT-PCR - (reply: 9)
  98. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
  99. mRNA Search for RT-PCR (U to T) - (reply: 2)
  100. RT-PCR primer - (reply: 3)
  101. Questions regarding RT-PCR optimization - (reply: 2)
  102. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
  103. Q-PCR and RT-PCR - (reply: 3)
  104. How long can diluted primary antibdoy remain effective at RT? - (reply: 7)
  105. RT-PCR result !! - (reply: 4)
  106. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
  107. cDNA storage and real time RT PCR - (reply: 3)
  108. Anitbiotic shelf life in liquid media at RT - (reply: 2)
  109. Question about RT... - (reply: 1)
  110. RT PCR for known snps detection? - SNPs detection (reply: 1)
  111. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)
  112. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
  113. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  114. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  115. use of housekeeping gene in RT PCR chIP - (reply: 3)
  116. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  117. Forgot to stop reverse transcription reaction - (reply: 4)
  118. RT-PCR contamination issue - (reply: 3)
  119. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  120. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  121. Dumb Reverse Transcription PCR Question - (reply: 2)
  122. Reverse transcription PCR - (reply: 1)
  123. PCR/RT-PCR beads - (reply: 2)
  124. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
  125. RT-PCR standard curve dilutions - (reply: 2)
  126. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  127. Embryo preservation for IHC at RT?? - (reply: 1)
  128. RT-PCR problem - (reply: 1)
  129. RT-PCR - New to RT-PCR info (reply: 2)
  130. silly control question - No RT controls (reply: 2)
  131. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  132. Relative RT-PCR, multiplate and calibration - (reply: 4)
  133. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
  134. Relative RT-PCR - (reply: 2)
  135. Relative real time RT PCR - (reply: 2)
  136. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
  137. How to do PCR to detect mRNA without RT? - (reply: 2)
  138. multiplexing taqman miRNA reverse transcription - (reply: 2)
  139. Problem cDNA synthesis/ 2 step RT - (reply: 6)
  140. Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
  141. Reverse transcription question - Volume for RT reaction (reply: 3)
  142. Antibody stability if left out at RT over Xmas break? - (reply: 5)
  143. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
  144. Does RT step smplify RNA - (reply: 2)
  145. reverse transcriptase and cDNA - (reply: 1)
  146. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  147. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  148. The perfect RT??? which control? - (reply: 4)
  149. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  150. RT-PCR problem - (reply: 4)
  151. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  152. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
  153. SYBR melting curve vs RT-PCR gel - (reply: 1)
  154. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  155. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  156. heating rna before rt reaction? - (reply: 1)
  157. Standard curve for RT-PCR - (reply: 1)
  158. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
  159. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 4)
  160. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  161. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
  162. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  163. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  164. RT-PCR Help - Protocol provided (reply: 4)
  165. RT qPCR Newbie, Weird Results - (reply: 1)
  166. truble shooting qiagen 1 step rt pcr kit - (reply: 2)
  167. Thiamine HCl 1M solution? - can I store it at RT? (reply: 2)
  168. RNA reverse transcription riddle - (reply: 6)
  169. Leaky RT-PCR - (reply: 4)
  170. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  171. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  172. amplification in negative control in RT PCR - (reply: 2)
  173. gene-specific RT-PCR - (reply: 4)
  174. RT-PCR Internal Standard - (reply: 2)
  175. RT-PCR - (reply: 3)
  176. RT-PCR vs. conventional PCR - (reply: 1)
  177. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  178. Gene-specific RT-PCR - (reply: 2)
  179. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  180. Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh - (reply: 3)
  181. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
  182. RT-PCR Negative controls - (reply: 1)
  183. Amplification dwindles using little template RT-PCR - (reply: 3)
  184. Running gel after RT-PCR - (reply: 5)
  185. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  186. gDNA or cDNA amplification in RT-PCR - (reply: 3)
  187. Reverse Transcription - (reply: 3)
  188. Need an UV260 RT-PCR instrument - (reply: 1)
  189. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  190. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  191. Serious issues with RT-PCR - (reply: 7)
  192. Analysis of ChIP RT-PCR data - (reply: 7)
  193. can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
  194. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  195. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  196. Ncode miRNA RT & qPCR system - can mRNA be reverse transcribed in same RT reaction? (reply: 4)
  197. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
  198. cDNA and RT-PCR - (reply: 8)
  199. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)
  200. RT-PCR - can you keep the PCR plate in the fridge? - Please help! (reply: 1)