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481. how to design pcr primers - (reply: 7)
482. RNA extraction from skin tissue - (reply: 2)
483. RT-PCR primer design and in silico PCR - (reply: 4)
484. DNA molar calculation - (reply: 3)
485. isolation of genomic DNA - (reply: 1)
486. RNA pellet can't dissolve in ethanol - (reply: 3)
487. how to know RNA concentration? - (reply: 4)
488. PCR yield, can assess by absorbance without electroforesis? - (reply: 2)
489. A260/280 - (reply: 3)
490. Checking mRNA expression by RT-PCR - (reply: 2)
491. Help with DIG UTP Pobe Construction In Vitro Transcription - (reply: 1)
492. Problem in RT-PCR - getting a band in the negative control (reply: 4)
493. methylation affects ligation? - (reply: 2)
494. DNA extraction vs purification - help ! (reply: 2)
495. Northern - really stuck here! - (reply: 3)
496. primer design - over expression (reply: 1)
497. Point Mutation, Protein Folding, Base Pairing - (reply: 3)
498. No mRNA or protein made by my mutant gene - (reply: 4)
499. Best Fluorescent Protein - (reply: 2)
500. qRT-PCR reproducibility of triplicates? - (reply: 6)
501. RT-PCR no amplification - (reply: 2)
502. Chromatin IP - What BSA to use for ChIP (reply: 1)
503. making INV110 competent cells - (reply: 1)
504. RNA isolation / Glycogen - (reply: 4)
505. Extracellular protein expression in E.coli - anyone knows a good commercial vector? (reply: 1)
506. quadraplex RT-PCR error - (reply: 1)
507. lux gene cloning using Sal I as restriction enzyme - (reply: 1)
508. RNA isolation from Chicken liver - (reply: 3)
509. nuclease activity assay - (reply: 1)
510. mRNA purification - (reply: 2)