5101. influence of MgCl2 in PCR - (reply: 7)
5103. cDNA quantification - (reply: 1)
5105. partial digestion - restriction conditions (reply: 2)
5107. Vector Vs Host - PGL2 Vs DH5alpha (reply: 1)
5109. Sequencing problems!! - homopolymeric region and slippage (reply: 8)
5111. Difference between Luria Agar and Luria-Bertani - Luria Agar and Luria-Bertani (reply: 2)
5113. please tell me how to eliminate interfering band ? - (reply: 1)
5115. gel stain and gel extraction - crystal violet (reply: 1)
5117. Chloroform-isoamyl extraction problem - Chloroform-isoamyl extraction problem (reply: 4)
5119. dNTPs - PCR (reply: 1)
5121. Problem in the Digestion - Bamh! and EcoR1 (reply: 3)
5123. Vector- Insert Ratio in Ligation - (reply: 2)
5125. Tripple Ligation - (reply: 7)
5127. Cloning into GFP vector - (reply: 2)
5129. Stable cell lines not overexpressing my gene of interest! - Technical problems (reply: 2)

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