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5461. single cell PCR - (reply: 8)
5462. Methylation and restriction digestion problem - (reply: 8)
5463. Annealing temperature for PCR - (reply: 3)
5464. poly(A)-RNA extraction from cells - (reply: 1)
5465. Invitrogen platinum pfx enzyme not so fantastic - problems with pcr product gel migration (reply: 1)
5466. inhibitor of glucose-6-phosphatase - help (reply: 1)
5467. DNA extraction - Is 2years ago extracted DNA still usable (reply: 2)
5468. PstI cutting - (reply: 3)
5469. Genomic DNA amplification - (reply: 4)
5470. Bands are like a "w" - (reply: 1)
5471. Ligation Reaction - (reply: 8)
5472. PCR works first time, then not! - (reply: 1)
5473. RT product check - Can I qualify my cDNA? (reply: 2)
5474. large fragment cloning - (reply: 2)
5475. wierd plasmids: maxi-prep never work - (reply: 13)
5476. Banding in agarose gel wells. - amplified fragment will not migrate! (reply: 7)
5477. A question about adenovirus infection 293 cells - (reply: 5)
5478. Cloning long fragments - (reply: 18)
5479. a question about mutagenesis - (reply: 5)
5480. RT-PCR, why two PCR steps? - two PCRs after reverse transcription?? (reply: 2)
5481. Annealing Temperatures - RT-PCR (reply: 1)
5482. total rna extraction from whole blood - for rt pcr (reply: 3)
5483. Does phenol affect Norhern blot? - need your advice (reply: 3)
5484. mRNA of bacteria - (reply: 1)
5485. DNA arrays for low gene expression ? - (reply: 1)
5486. PCR is6110 Tuberculosis - Non specific amplification (reply: 2)
5487. MC57G cells and transfection - (reply: 1)
5488. Na2Hpo4 , NaH2po4 or both in Church buffer ?! - (reply: 2)
5489. PCR from BAC - (reply: 1)
5490. Enhance specificity of 5´Race - (reply: 1)