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5011. How to increase electroporation efficiency? Please help (long!) - (reply: 2)
5012. southern hybridization temperature - (reply: 2)
5013. dna extraction from soil - (reply: 4)
5014. designing gene-specific primer for cDNA synthesis - (reply: 5)
5015. question on Northern blotting - (reply: 3)
5016. Determine the RNA concentraiton for linear PCR - (reply: 1)
5017. Klenow or T4 PNK to fill in? - (reply: 7)
5018. Purification of small (100bp) PCR products - (reply: 7)
5019. Primer design for unknown gene - (reply: 4)
5020. ligation problem - self ligation of vector - (reply: 5)
5021. TOPO TA pCR 2.1 cloning - (reply: 1)
5022. RACE artifacts - (reply: 1)
5023. Ligate two restriction fragments of dsDNA - (reply: 6)
5024. Blunting both vector and insert - klenow and CIPping (reply: 2)
5025. Sequential digestion with NheI and XhoI - Restrictive Digestion (reply: 3)
5026. Help!What's wrong with the endonuclease digestion of total DNA of - (reply: 2)
5027. CAT assay - WTF? (reply: 4)
5028. Gene expression - inducible Gene expression (reply: 1)
5029. problems purifying plasmid DNA after restriction digestion - (reply: 3)
5030. Agarose Gel Electrophoresis of plasmid DNA - Agarose Gel Electrophortesis of plasmid DNA (reply: 1)
5031. Question about cloning a gene coding an enzyme - (reply: 1)
5032. dsOligos annealing buffer - (reply: 2)
5033. Recombinant DNA - Problems with ligation (reply: 1)
5034. Leucine-rich repeats - (reply: 1)
5035. Real-time PCR problems - (reply: 3)
5036. Cloning rare splice variants - Molecular Biology (reply: 1)
5037. small sized restricted bands couldnt be visualized - restriction enzymes problems (reply: 22)
5038. Site Directed Mutagenesis Problem - (reply: 5)
5039. DNA purification kit for Microinjection - Anyone trying to purify fragements around 45 kb? (reply: 1)
5040. pQE30 Vs pET - Which is the better? (reply: 4)