Protocol Online logo
Top : Forum Archives: : Molecular-Biology
61. DNase removal prior to mRNA isolation - (reply: 4)
62. RNA extraction from frozen cells suspended in PBS - (reply: 2)
63. RNA clean up - RNA clean up (reply: 4)
64. RNA samples precipitates when I add 10X Buffer for Turbo Dnase treatment - RNA precipitation (reply: 2)
65. PCR disappeared - there is no amplification (reply: 11)
66. help for the meaning - pSHY2::shy2 and pSHY2:SHY2 (reply: 3)
67. plasmid preparation - (reply: 10)
68. double digestion time - (reply: 1)
69. Can I store DEPC water at -20? - I made 1mL aliquots of DEPC water. Can I freeze them? (reply: 2)
70. promoter cloning - (reply: 1)
71. cloning past stop codon - 3'end stop codon cloning (reply: 1)
72. deisgning primers with restriction site - (reply: 7)
73. MMLV RT - (reply: 2)
74. heating RNA in water - (reply: 1)
75. Plasmid isolation from bioluminescence bacteria - Alkaline lysis (reply: 5)
76. pGL-3 vector transformation - (reply: 5)
77. RNA, cDNA, PCR - RT Inconsistency - High Quality RNA, Working RT, Varying PCR Results (reply: 2)
78. RT-PCR works but not consistent - (reply: 3)
79. cDNA and primer Design Help - (reply: 6)
80. antibiotic stability in plates at room temperature? - (reply: 2)
81. inoculating liquid media from frozen cell stock ok? - (reply: 6)
82. RT-PCR - low mol.wt band (reply: 2)
83. Background amplification - (reply: 1)
84. tagging of gene with GUS gene - (reply: 1)
85. Toxicity of Transfection -> Endotoxin? - (reply: 4)
86. Should I chuck my competent cells? probably contaminated.. - (reply: 4)
87. dNTPS concentration - (reply: 2)
88. RFLP - BsaJ1 (reply: 3)
89. How to revive bacteria that are shipped on dry ice - (reply: 1)
90. QC in RNA extraction - Low A260/A230 ratio (reply: 2)