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2011. Transfection of expression vector for gel shift - how much vector should be transfected? (reply: 2)
2012. plasmid isolation help urgent.. - (reply: 4)
2013. dissolving DNA - (reply: 3)
2014. Long and Accurate PCR - (reply: 2)
2015. Identification by 16S rDNA - using short primer (reply: 2)
2016. PCR stopped working with new dNTP! - (I've tried all the usual suspects) (reply: 4)
2017. Trick in competent cell - make me crazy (reply: 8)
2018. pGM-T vector problem - (reply: 3)
2019. how to amplify a long gene? - (reply: 3)
2020. how to see splicing.. - what do you think about what I planned? (reply: 1)
2021. Positive control doesn’t work, sample does, but negative control still negative - I need help fast (reply: 6)
2022. DB3.1 resistance - (reply: 3)
2023. RNase treatment - genomic DNA sample lost during purification - RNase treatment troubleshooting (reply: 1)
2024. NTSYS Software for analysis - (reply: 1)
2025. STAT-3/phosphoSTAT-3 western blot - (reply: 6)
2026. PCR buffer and PCR efficiency - buffer pH and salt (reply: 2)
2027. How to get the double strand cDNA from bacterial - (reply: 1)
2028. yeast genomic DNA extraction - (reply: 2)
2029. Invitrogen GeneTailor Site-Directed Mutagenesis Reaction--HELP! - (reply: 1)
2030. sequencing - (reply: 3)
2031. lysing yeast - (reply: 2)
2032. TA cloning - (reply: 6)
2033. Protocols for RNA extraction from 5-10mg starting tissue samples - Molecular Biology (reply: 1)
2034. cell concentration calculation - (reply: 2)
2035. size of an endothelial cell - (reply: 2)
2036. Restriction digestion - (reply: 2)
2037. RNA extraction - didnt get ratio 2:1 ( 28s rRNA : 18 s rRNA) - RNA extraction from Tilapia (reply: 2)
2038. Screening of Mouse BAC Clone Libraries - Molecular Biology (reply: 2)
2039. restriction enzymes primers - (reply: 5)
2040. No virus titer and MSCV-mir30 vector problem - HELP (reply: 12)