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1411. how to explain this strange amplification chart? - the Ct value comes to be very low because of the unusual amplification (reply: 1)
1412. Insert only ligating in backward direction - (reply: 2)
1413. PCR Troubleshooting - (reply: 2)
1414. Transformation problem with newly ligated plasmids - (reply: 1)
1415. cDNA - amonut to use (reply: 3)
1416. RNA-chip - (reply: 1)
1417. Sequence editing - (reply: 1)
1418. Formaldehyde Gel Electrophoresis on non-denatured/denatured RNA - No/Faint RNA bands after denatration (reply: 1)
1419. My dot blot hybridization doesn't work! - (reply: 21)
1420. EMSA probe: two bands in free probe lane - (reply: 1)
1421. Changing Valine to Leucine - (reply: 1)
1422. Nested PCR - (reply: 5)
1423. DNAse I - Degrading my DNA (reply: 2)
1424. Need help about my subcloning pET15b. - (reply: 1)
1425. recipe for SB buffer for electrophoresis - (reply: 4)
1426. i dont get colonies - (reply: 3)
1427. primer design for PCR cloning - (reply: 1)
1428. how to make a sucrose gradient? - (reply: 1)
1429. What is the suitable product size for conventional RT-PCR analysis - products with size over 1 kb? (reply: 6)
1430. in vitro synthesis of RNA using T7 - (reply: 2)
1431. annealing s.s. DNA - (reply: 2)
1432. deletion PCR? - I need help (reply: 1)
1433. Differences seen in regular and real time machine - (reply: 2)
1434. Eukaryotic recombinant protein expression - (reply: 3)
1435. your experience with long range PCR - (reply: 3)
1436. Open Biosystems - anyone use thier libraries? (reply: 4)
1437. circularized mRNA for translation - Does the mRNA relinearize after large subunit docking? (reply: 1)
1438. PCR troubles - PCR works one day but not the next (reply: 10)
1439. short oligos ligation and transformation - (reply: 3)
1440. What % identity is enough between probe and template? - (reply: 2)