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121. DNA extraction from Filters with UltraClean Soil kit. - (reply: 2)
122. IPTG induction in phage display techniques - (reply: 2)
123. PCR of ssDNA - (reply: 2)
124. How to extract DNA for PFGE - (reply: 2)
125. DNA extraction from Fruit pulp - HELP !!!! (reply: 2)
126. Problems dissolving TriZol RNA pellet - (reply: 5)
127. genomic DNA and cDNA - question (reply: 4)
128. Analysing media - (reply: 2)
129. The vector became smaller - (reply: 4)
130. primer design software - primes for full lenth gene (reply: 3)
131. pcr amplification 5 kb - (reply: 4)
132. NF-kB binding activity in neutrophils and mononuclear cells - (reply: 1)
133. problem in X-gal plate - (reply: 2)
134. poplar - (reply: 2)
135. Bacterial culture - (reply: 8)
136. How to identify unknown sequence? - No BLAST identity!! (reply: 21)
137. Why DNA sequencing does not detect deletions - help! - Med student lost on her hw. Breast cancer gene (BRCA1) mutations (reply: 3)
138. eukaryotic inducible vector with GFP-tag fusion? - (reply: 3)
139. How to know the genome size? - (reply: 4)
140. contmination PCR? - bands in - control but no bands in samples (reply: 2)
141. Does PFU creat overhangs - (reply: 2)
142. C and N terminal - which is extracellular and which is intracellular (reply: 7)
143. RNA conc importance? - (reply: 2)
144. problem with phase separation using Tri-reagent - (reply: 3)
145. HSV1-Tk (mono/ployclonal) antibody. - (reply: 1)
146. Wnt/b-catenin pathway - LEf/TCF transcription activity (reply: 1)
147. how to find promoter - (reply: 3)
148. Sequencing non-existent DNA? - (reply: 9)
149. Tm of the primers - company sends primers with lower Tm than expected (reply: 3)
150. cDNA synthesis - (reply: 3)