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3601. Ligation Problems - (reply: 5)
3602. LB + Amp. preparation - Recipes (reply: 6)
3603. phenol Vs salting out - (reply: 1)
3604. BclI digestion - (reply: 4)
3605. ridiculous problem - protein purification (reply: 2)
3606. ligating adaptor in 26kb plasmid - (reply: 1)
3607. Platinum Taq, 3' A and proofreading - (reply: 6)
3608. No plasmid in HB101 - whereas DH5alpha does (reply: 2)
3609. protein purification - preserve pellet (reply: 2)
3610. blunting with no success! - blunt end ligation (reply: 1)
3611. TOPO TA cloning - (reply: 2)
3612. Plasmid purification - (reply: 5)
3613. polyA before IRES - bad or OK? - (reply: 6)
3614. Vector - Proteins Folding (reply: 2)
3615. Impact of temperature and time increment during PCR - (reply: 3)
3616. Digestion Problem - Cloning (reply: 5)
3617. Multiplex Primers - Primer primer interaction (reply: 1)
3618. PRoduct lenght determination - product length determination (reply: 2)
3619. Agarose gel - DEPC-induced fragility? - RNA gels (reply: 2)
3620. pCMV-CD19 plasmid map - (reply: 2)
3621. Housekeeping gene for RT-PCR on plant - RT PCR (reply: 1)
3622. inconsistent PCR results - (reply: 2)
3623. topo XL cloning - (reply: 5)
3624. PCR smear in samples and negative control - (reply: 5)
3625. Protein imputities in extracted genomic DNA - (reply: 1)
3626. Is it necessary to treat total RNA with DNAse I when isolating mRNA? - (reply: 16)
3627. Problem in RNA isolation, degraded, even though I pay extra attention to prevent - (reply: 14)
3628. pcr sequencing - (reply: 2)
3629. DNA extraction from oilseed plants - (reply: 3)
3630. Exon intron boundaries - where to check those (reply: 3)