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RNAi Related Discussions
  1. BLOCK-iT Pol II miR RNAi system: Issues with generating stable knockdown - (reply: 1)
  2. non-radioactive detection of siRNA in plants - (reply: 4)
  3. Subsequent DNA and siRNA transfection - (reply: 8)
  4. A help for siRNA experiment - (reply: 2)
  5. siRNA detection......which strand shuld labelled - (reply: 2)
  6. siRNA quantification using UV - (reply: 1)
  7. overexpress mature microRNA by siRNA vector - (reply: 2)
  8. siRNA Agarose gel electrophoresis - Protocol - (reply: 1)
  9. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
  10. Apoptosis inducing siRNA? - (reply: 1)
  11. siRNA in E.Coli - (reply: 2)
  12. siRNA resuspension in RNase-free water - (reply: 1)
  13. Wound Scratch Assay using siRNA - (reply: 4)
  14. Recommendation on siRNA transfection reagents - (reply: 2)
  15. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
  16. Problems in RNAi construction by using pUC19 - (reply: 3)
  17. DNA and siRNA transfection and cytotoxicity - (reply: 1)
  18. Hiperfect siRNA in HT-29 cells help - (reply: 2)
  19. SF9 transfection with siRNA - what transfection reagent has worked for you? - (reply: 3)
  20. siRNA modifications - (reply: 2)
  21. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
  22. transfecting siRNA thp-1 after differentiated to macrophage - (reply: 2)
  23. Converting shRNA sequence to siRNA - (reply: 1)
  24. target mRNA/protein levels increase with siRNA - (reply: 2)
  25. One siRNA sequence, many cell lines - (reply: 1)
  26. RNAi in combination with adenoviral-mediated over-expression - (reply: 1)
  27. Need an effective siRNA/ shRNA sequence for downregulating caveolin-1 - (reply: 2)
  28. Higher mRNA level after RNAi? - (reply: 1)
  29. question: RNAi for bacteria? - (reply: 3)
  30. RNAi problem - Gene is knocked down, but not protein - (reply: 8)
  31. Calculating siRNA Transfection Efficiency - (reply: 3)
  32. siRNA time - (reply: 1)
  33. New RNAi video from Nature - (reply: 1)
  34. What to do Post-transfection of cells with siRNA? - (reply: 2)
  35. Difference between <Antisense RNA> & <RNAi>? - (reply: 1)
  36. Which company to buy siRNA from? - (reply: 1)
  37. placing siRNA seq into miRNA stem-loop - (reply: 4)
  38. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
  39. problem with siRNA transfection of HEK cells - (reply: 5)
  40. Decreased siRNA performance at high concentrations: feasible? - (reply: 1)
  41. Phenotypes with two different siRNA but no knockdown? - trouble in showing a knock-down (reply: 2)
  42. siRNA transfection advise - Need some help on siRNA knockdowns (reply: 1)
  43. siRNA optimization - (reply: 5)
  44. siRNA transfection with lipofectamine 2000 - (reply: 1)
  45. Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
  46. siRNA screening and RNAse free - (reply: 1)
  47. miRNA/siRNA transfection doubts - (reply: 2)
  48. siRNA transfection - 7-day study - serum free media? (reply: 2)
  49. siRNA shearing during light sonication? - (reply: 1)
  50. RNAi showing upregulation via QPCR - Help needed (reply: 3)
  51. siRNA DNA co-transfection - (reply: 3)
  52. RNAi is somatic and germline cells - (reply: 3)
  53. Complete siRNA newbie!! - Muddling myself up!! (reply: 6)
  54. siRNA and Fugene6 - (reply: 1)
  55. how to check if siRNA is intact - (reply: 1)
  56. siRNA in vivo delivery - (reply: 2)
  57. siRNA newb - How to go about starting an siRNA project (reply: 3)
  58. Weird effect of siRNA on protein in cells - (reply: 3)
  59. siRNA to shRNA...trouble! - (reply: 1)
  60. How to Design a Negative Control siRNA - (reply: 1)
  61. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
  62. Importin alpha siRNA - Looking for siRNA for knockdown of all Importin alpha subtypes (reply: 2)
  63. siRNA overhangs - (reply: 2)
  64. siRNA in the Fridge - (reply: 2)
  65. Double transfection of siRNA - (reply: 2)
  66. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  67. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
  68. Designing siRNA from Sequence provided by Qiagen - How to design from a given Nucleotide sequence a siRNA?? (reply: 4)
  69. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
  70. siRNA againts Beta Actin Help PLease - (reply: 2)
  71. cell cryopreservation after siRNA transfection - (reply: 1)
  72. Luciferase GL4 siRNA - (reply: 5)
  73. siRNA staining? - (reply: 1)
  74. Question about the control for an RNAi Experiment - (reply: 3)
  75. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  76. Verified RNAi Sequences - (reply: 4)
  77. siRNA and plasmid cotransfection - (reply: 1)
  78. design parameters for multiple siRNA expression in vector - (reply: 4)
  79. siRNA with low mRNA levels - (reply: 7)
  80. si-Fi, Software for RNAi off-target prediction - (reply: 2)
  81. siRNA electroporation - (reply: 1)
  82. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
  83. Is it possible to reuse electroporation cuvette to transfect siRNA - (reply: 4)
  84. Silencer® GFP (eGFP) siRNA – AM4626 - (reply: 2)
  85. HepG2 siRNA transfection - (reply: 1)
  86. siRNA - (reply: 2)
  87. in vitro siRNA - wich is the best company for a siRNA for in vitro assay? (reply: 2)
  88. RNAi - (reply: 1)
  89. protein complex and siRNA - (reply: 2)
  90. Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
  91. why using siRNA knock down,but get the mRNA products? - (reply: 3)
  92. Designing new RNAi - (reply: 2)
  93. siRNA on primary DRG cell - transfection agent/protocol (reply: 3)
  94. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  95. So confused with RNAi - (reply: 3)
  96. RNAi in Zebrafish - vector for zebrafish knock-down (reply: 4)
  97. siRNA concentration in divinding-growing cells - (reply: 2)
  98. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
  99. siRNA resistant gene - How many bp to change? (reply: 4)
  100. siRNA cell function examples - (reply: 1)
  101. Good SiRNA Gene silencing - (reply: 1)
  102. cellular siRNA localization - (reply: 4)
  103. Define Trasnsfection efficiency interms of siRNA experiment - Sirna uptake Vs transfection efficiency (reply: 12)
  104. Amaxa siRNA - (reply: 3)
  105. Old threads on "siRNA, microRNA and RNAi" are achived here - (reply: 3)
  106. Identification of miRNA and siRNA - (reply: 1)
  107. RNAi in bacteria - Why no finding for gene silencing in bacteria? (reply: 1)