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E. Coli Related Discussions
  1. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
  2. Identifying unknown e. coli - (reply: 3)
  3. Affordable source of LB broth for e.coli storage? - (reply: 8)
  4. Proper source of ampicillin for e.coli growth medium? - (reply: 2)
  5. Antibody against E.coli lysate - (reply: 1)
  6. Efficient lysis of E.coli cells - (reply: 4)
  7. Differences between DH5a strain of E. Coli and BL21 strain of E.Coli - (reply: 2)
  8. Expression of plant protein in Ecoli - (reply: 1)
  9. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
  10. E coli ST131 - (reply: 2)
  11. Transformation E.Coli help - (reply: 2)
  12. Collagen binding protein expression E. coli His-tag - (reply: 1)
  13. TOP10F Chemically Competent E. coli - (reply: 1)
  14. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
  15. Can't isolate plasmid from cloned E.coli - (reply: 4)
  16. Screen for F plasmid in e. coli - (reply: 1)
  17. vector propagation in E coli - (reply: 1)
  18. E coli culture cannot grow overnight. - (reply: 11)
  19. genotype of e coli strain for protein expression - (reply: 2)
  20. Identification of L.Mono and E.coli - (reply: 4)
  21. Fluoroquinolone resistant ecoli - (reply: 1)
  22. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
  23. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  24. Expression in different E.coli strains - (reply: 3)
  25. Maintaining E. Coli (ATCC 25922) - (reply: 5)
  26. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
  27. E coli. and Staph identification problem - (reply: 1)
  28. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
  29. E. coli culture - (reply: 1)
  30. E coli, bacterial strain problem questions - (reply: 2)
  31. Phosphorylation of recombinant proteins in E. coli - (reply: 1)
  32. production of phosphorylated proteins in E.coli host system - (reply: 1)
  33. Selection of E.coli competent cells - (reply: 2)
  34. E. coli (ATCC 25922) growth in Minimal Medium - (reply: 5)
  35. Cloning cDNA construct into E.Coli. - (reply: 4)
  36. Grow multiple Ecoli colonies in a single liquid media tube - (reply: 4)
  37. Plasmid stability in Ecoli during storage - (reply: 3)
  38. Problems in expression of target protein in E. coli - (reply: 3)
  39. reference gene for qPCR in E.coli - (reply: 2)
  40. How to select competent E. coli cells? - (reply: 7)
  41. heterologous gene integration into e.coli genome - (reply: 1)
  42. Very small peptide expression in E. coli - (reply: 2)
  43. Continuous growth of E. coli on AMP LB plate - (reply: 2)
  44. Protein expression in e.coli - (reply: 5)
  45. PTM in E. coli - (reply: 1)
  46. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  47. siRNA in E.Coli - (reply: 2)
  48. Protein Extraction: Extremely Sticky e coli lysate - (reply: 1)
  49. Problems with Ecoli glycerol stock - (reply: 6)
  50. Unable to clone in E. coli cells - (reply: 3)
  51. Cleaning/eliminating E.coli adsorbed onto substrates - (reply: 1)
  52. SURE E. coli competent cells - (reply: 1)
  53. E. coli growing on plates and bot not in LB medium - (reply: 10)
  54. Dam-/+ e. coli strains - (reply: 6)
  55. E. coli strain for conjugation - (reply: 1)
  56. pkD46 vector growth in E.coli problem - (reply: 8)
  57. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  58. Polymerase for E. coli screenings - (reply: 5)
  59. Unknown contaminant in Ecoli culture - (reply: 4)
  60. Would E. Coli cells be stable at room temp, if in media with glycerol? - (reply: 1)
  61. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
  62. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  63. How to insert genes into chromosome of E. coli - (reply: 1)
  64. e coli vector and host selection - (reply: 1)
  65. E.coli grow on neg. control - (reply: 1)
  66. Co-expression of 2 plasmids in E.coli - (reply: 1)
  67. Ecoli bacteria storage - (reply: 3)
  68. transporting E coli on filter paper - (reply: 5)
  69. Problem with Transforming E. coli DH10Bac - (reply: 8)
  70. Plasmid vector for yeast and E. coli - (reply: 2)
  71. E. coli cells refuse to grow! - (reply: 2)
  72. blast bacterial genomes like e.coli - (reply: 1)
  73. What OD600 is suitable for assaying Ecoli viability - (reply: 9)
  74. ask for a strain for the suicide vector called E. coli S17-1 pir - (reply: 1)
  75. What do you expect to see in E. coli digested with EcoRI? - (reply: 8)
  76. Fermentation with E.coli XL10gold - (reply: 9)
  77. gene expression in E coli with PET21a vector - (reply: 1)
  78. co-expression of 2 plasmids in E.coli - (reply: 3)
  79. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
  80. protocol for making e.coli competent cells under low temperature - (reply: 1)
  81. Any competent or certain E Coli for mutagenesis transformation? - (reply: 1)
  82. No activity of fungi protein in E.coli - (reply: 15)
  83. Dropped e.coli (BL21) culture on clothes - (reply: 3)
  84. e.coli growth curve in microtiter plate - (reply: 1)
  85. protein expression level and OD600 in E.coli - (reply: 1)
  86. Strange E.coli growth - (reply: 3)
  87. stop codon readthrough in E.coli - (reply: 8)
  88. How to improve protein solubility in E. coli? - (reply: 11)
  89. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
  90. Upscaling protein expression in E.coli - (reply: 2)
  91. E coli K 12 - (reply: 1)
  92. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  93. E.coli cells lyse in minimal media - (reply: 3)
  94. e coli plate left too long at 37C - very small clones appeared (reply: 4)
  95. How much slower does E coli grow in M9 vs LB? - (reply: 2)
  96. Growth conditions for E. coli DB3.1 cells - (reply: 1)
  97. complementing a double knockout in E. coli - (reply: 1)
  98. Chemical competent E.coli always from single colony ? - (reply: 2)
  99. Expressing human protein in E.Coli - (reply: 12)
  100. Detergent-based lysis buffer reciipe for E coli - (reply: 6)
  101. growth of E.coli in minimal media(m9) is very less - growth of E.coli in minimal media(m9) is very less (reply: 2)
  102. E.Coli glycerol stock - (reply: 1)
  103. Cheap procedure for gene deletion in E coli? - (reply: 2)
  104. recombinant ecoli in log phase overnight media suggestion - (reply: 2)
  105. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  106. E coli CFUs - 48h incubation - silly Q! (reply: 2)
  107. Alcohol effect on genetic manipulated E.coli. - (reply: 1)
  108. Differentiating Ecoli from Agrobacteria quickly. - Noob mistake, I am paying for now. (reply: 2)
  109. E.coli suddenly death?? - (reply: 6)
  110. E.Coli growth at 4 degrees? - (reply: 8)
  111. Inclusion bodies in e.coli - (reply: 1)
  112. lysis of e.coli by sonication - (reply: 2)
  113. E. coli not growing in liquid cultures :( - (reply: 4)
  114. E.coli expression - (reply: 5)
  115. E coli growth at 30 C - (reply: 2)
  116. E coli lysis by lysozyme - (reply: 2)
  117. Pellet Formation - pellet formation for different concentrations of e.coli (reply: 1)
  118. e coli strain selection for protein expression - (reply: 2)
  119. e.coli contamination - (reply: 2)
  120. do e.coli cells get ruptured during high spin - (reply: 1)
  121. E. Coli culture in microplate - (reply: 5)
  122. functionality of yeast promoter in E. coli - (reply: 1)
  123. Good antibody for E. coli surface marker - phagocytosis assay (reply: 2)
  124. E coli transformation - (reply: 8)
  125. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
  126. Incompatible restriction sites - Will E.coli ligate for me? (reply: 2)
  127. How to ask the author for an E. coli strains used in his paper? - (reply: 6)
  128. ccdB survival E. coli transformation - (reply: 2)
  129. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
  130. cempetent e.coli and transformation - (reply: 2)
  131. 4 reasons why E.coli is selected as indicator of water potability? - *I've tried few* (reply: 3)
  132. prepare sample protein for 2d from E.coli - (reply: 2)
  133. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  134. Picking strain for e coli competent cells - (reply: 6)
  135. Removal of Methionine in E.coli - (reply: 1)
  136. E.Coli expressing GFP - (reply: 4)
  137. Amount of inoculum? - for protein expression in E.coli (reply: 3)
  138. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  139. Best way to resuspend E. coli? - (reply: 7)
  140. look for E.coli DB3.1 bacteria - (reply: 2)
  141. Plasmid instability in E.coli BL21? - (reply: 3)
  142. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  143. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
  144. Phage removal of E.coli - (reply: 8)
  145. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  146. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
  147. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  148. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  149. freezing of E.coli culture prior DNA prep - (reply: 5)
  150. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
  151. Yellow E. coli colonies on MACCONKEY AGAR - (reply: 2)
  152. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
  153. Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
  154. Mammalian protein expression in E. coli - (reply: 1)
  155. Bacterial conjugation - between E. coli and Salmonella Typhimurium (reply: 1)
  156. E. coli Strains - (reply: 4)
  157. Multiple colony types from streaking 1 E.coli colony - (reply: 5)
  158. What's the best temperature for killing E.coli? - (reply: 4)
  159. making competent E coli DH5a cells - (reply: 6)
  160. E. coli colonies didn't grow up in liquid culture. -HELP- - (reply: 9)