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E. Coli Related Discussions
  1. slimy cell paste of recombinant e.coli - (reply: 3)
  2. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
  3. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
  4. Identifying unknown e. coli - (reply: 3)
  5. Affordable source of LB broth for e.coli storage? - (reply: 8)
  6. Proper source of ampicillin for e.coli growth medium? - (reply: 2)
  7. Antibody against E.coli lysate - (reply: 1)
  8. Efficient lysis of E.coli cells - (reply: 4)
  9. Differences between DH5a strain of E. Coli and BL21 strain of E.Coli - (reply: 2)
  10. Expression of plant protein in Ecoli - (reply: 1)
  11. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
  12. E coli ST131 - (reply: 2)
  13. Transformation E.Coli help - (reply: 2)
  14. Collagen binding protein expression E. coli His-tag - (reply: 1)
  15. TOP10F Chemically Competent E. coli - (reply: 1)
  16. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
  17. Can't isolate plasmid from cloned E.coli - (reply: 4)
  18. Screen for F plasmid in e. coli - (reply: 1)
  19. vector propagation in E coli - (reply: 1)
  20. E coli culture cannot grow overnight. - (reply: 11)
  21. genotype of e coli strain for protein expression - (reply: 2)
  22. Identification of L.Mono and E.coli - (reply: 4)
  23. Fluoroquinolone resistant ecoli - (reply: 1)
  24. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
  25. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  26. Expression in different E.coli strains - (reply: 3)
  27. Maintaining E. Coli (ATCC 25922) - (reply: 5)
  28. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
  29. E coli. and Staph identification problem - (reply: 1)
  30. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
  31. E. coli culture - (reply: 1)
  32. E coli, bacterial strain problem questions - (reply: 2)
  33. Phosphorylation of recombinant proteins in E. coli - (reply: 1)
  34. production of phosphorylated proteins in E.coli host system - (reply: 1)
  35. Selection of E.coli competent cells - (reply: 2)
  36. E. coli (ATCC 25922) growth in Minimal Medium - (reply: 5)
  37. Cloning cDNA construct into E.Coli. - (reply: 4)
  38. Grow multiple Ecoli colonies in a single liquid media tube - (reply: 4)
  39. Plasmid stability in Ecoli during storage - (reply: 3)
  40. Problems in expression of target protein in E. coli - (reply: 3)
  41. reference gene for qPCR in E.coli - (reply: 2)
  42. How to select competent E. coli cells? - (reply: 7)
  43. heterologous gene integration into e.coli genome - (reply: 1)
  44. Very small peptide expression in E. coli - (reply: 2)
  45. Continuous growth of E. coli on AMP LB plate - (reply: 2)
  46. Protein expression in e.coli - (reply: 5)
  47. PTM in E. coli - (reply: 1)
  48. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  49. siRNA in E.Coli - (reply: 2)
  50. Protein Extraction: Extremely Sticky e coli lysate - (reply: 1)
  51. Problems with Ecoli glycerol stock - (reply: 6)
  52. Unable to clone in E. coli cells - (reply: 3)
  53. Cleaning/eliminating E.coli adsorbed onto substrates - (reply: 1)
  54. SURE E. coli competent cells - (reply: 1)
  55. E. coli growing on plates and bot not in LB medium - (reply: 10)
  56. Dam-/+ e. coli strains - (reply: 6)
  57. E. coli strain for conjugation - (reply: 1)
  58. pkD46 vector growth in E.coli problem - (reply: 8)
  59. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  60. Polymerase for E. coli screenings - (reply: 5)
  61. Unknown contaminant in Ecoli culture - (reply: 4)
  62. Would E. Coli cells be stable at room temp, if in media with glycerol? - (reply: 1)
  63. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
  64. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  65. How to insert genes into chromosome of E. coli - (reply: 1)
  66. e coli vector and host selection - (reply: 1)
  67. E.coli grow on neg. control - (reply: 1)
  68. Co-expression of 2 plasmids in E.coli - (reply: 1)
  69. Ecoli bacteria storage - (reply: 3)
  70. transporting E coli on filter paper - (reply: 5)
  71. Problem with Transforming E. coli DH10Bac - (reply: 8)
  72. Plasmid vector for yeast and E. coli - (reply: 2)
  73. E. coli cells refuse to grow! - (reply: 2)
  74. blast bacterial genomes like e.coli - (reply: 1)
  75. What OD600 is suitable for assaying Ecoli viability - (reply: 9)
  76. ask for a strain for the suicide vector called E. coli S17-1 pir - (reply: 1)
  77. What do you expect to see in E. coli digested with EcoRI? - (reply: 8)
  78. Fermentation with E.coli XL10gold - (reply: 9)
  79. gene expression in E coli with PET21a vector - (reply: 1)
  80. co-expression of 2 plasmids in E.coli - (reply: 3)
  81. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
  82. protocol for making e.coli competent cells under low temperature - (reply: 1)
  83. Any competent or certain E Coli for mutagenesis transformation? - (reply: 1)
  84. No activity of fungi protein in E.coli - (reply: 15)
  85. Dropped e.coli (BL21) culture on clothes - (reply: 3)
  86. e.coli growth curve in microtiter plate - (reply: 1)
  87. protein expression level and OD600 in E.coli - (reply: 1)
  88. Strange E.coli growth - (reply: 3)
  89. stop codon readthrough in E.coli - (reply: 8)
  90. How to improve protein solubility in E. coli? - (reply: 11)
  91. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
  92. Upscaling protein expression in E.coli - (reply: 2)
  93. E coli K 12 - (reply: 1)
  94. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  95. E.coli cells lyse in minimal media - (reply: 3)
  96. e coli plate left too long at 37C - very small clones appeared (reply: 4)
  97. How much slower does E coli grow in M9 vs LB? - (reply: 2)
  98. Growth conditions for E. coli DB3.1 cells - (reply: 1)
  99. complementing a double knockout in E. coli - (reply: 1)
  100. Chemical competent E.coli always from single colony ? - (reply: 2)
  101. Expressing human protein in E.Coli - (reply: 12)
  102. Detergent-based lysis buffer reciipe for E coli - (reply: 6)
  103. growth of E.coli in minimal media(m9) is very less - growth of E.coli in minimal media(m9) is very less (reply: 2)
  104. E.Coli glycerol stock - (reply: 1)
  105. Cheap procedure for gene deletion in E coli? - (reply: 2)
  106. recombinant ecoli in log phase overnight media suggestion - (reply: 2)
  107. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  108. E coli CFUs - 48h incubation - silly Q! (reply: 2)
  109. Alcohol effect on genetic manipulated E.coli. - (reply: 1)
  110. Differentiating Ecoli from Agrobacteria quickly. - Noob mistake, I am paying for now. (reply: 2)
  111. E.coli suddenly death?? - (reply: 6)
  112. E.Coli growth at 4 degrees? - (reply: 8)
  113. Inclusion bodies in e.coli - (reply: 1)
  114. lysis of e.coli by sonication - (reply: 2)
  115. E. coli not growing in liquid cultures :( - (reply: 4)
  116. E.coli expression - (reply: 5)
  117. E coli growth at 30 C - (reply: 2)
  118. E coli lysis by lysozyme - (reply: 2)
  119. Pellet Formation - pellet formation for different concentrations of e.coli (reply: 1)
  120. e coli strain selection for protein expression - (reply: 2)
  121. e.coli contamination - (reply: 2)
  122. do e.coli cells get ruptured during high spin - (reply: 1)
  123. E. Coli culture in microplate - (reply: 5)
  124. functionality of yeast promoter in E. coli - (reply: 1)
  125. Good antibody for E. coli surface marker - phagocytosis assay (reply: 2)
  126. E coli transformation - (reply: 8)
  127. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
  128. Incompatible restriction sites - Will E.coli ligate for me? (reply: 2)
  129. How to ask the author for an E. coli strains used in his paper? - (reply: 6)
  130. ccdB survival E. coli transformation - (reply: 2)
  131. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
  132. cempetent e.coli and transformation - (reply: 2)
  133. 4 reasons why E.coli is selected as indicator of water potability? - *I've tried few* (reply: 3)
  134. prepare sample protein for 2d from E.coli - (reply: 2)
  135. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  136. Picking strain for e coli competent cells - (reply: 6)
  137. Removal of Methionine in E.coli - (reply: 1)
  138. E.Coli expressing GFP - (reply: 4)
  139. Amount of inoculum? - for protein expression in E.coli (reply: 3)
  140. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  141. Best way to resuspend E. coli? - (reply: 7)
  142. look for E.coli DB3.1 bacteria - (reply: 2)
  143. Plasmid instability in E.coli BL21? - (reply: 3)
  144. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  145. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
  146. Phage removal of E.coli - (reply: 8)
  147. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  148. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
  149. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  150. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  151. freezing of E.coli culture prior DNA prep - (reply: 5)
  152. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
  153. Yellow E. coli colonies on MACCONKEY AGAR - (reply: 2)
  154. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
  155. Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
  156. Mammalian protein expression in E. coli - (reply: 1)
  157. Bacterial conjugation - between E. coli and Salmonella Typhimurium (reply: 1)
  158. E. coli Strains - (reply: 4)
  159. Multiple colony types from streaking 1 E.coli colony - (reply: 5)
  160. What's the best temperature for killing E.coli? - (reply: 4)
  161. making competent E coli DH5a cells - (reply: 6)
  162. E. coli colonies didn't grow up in liquid culture. -HELP- - (reply: 9)