Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
E. Coli Related Discussions
  1. activity optimalisation of proteinase K by E. coli - (reply: 1)
  2. slimy cell paste of recombinant e.coli - (reply: 3)
  3. An unusual "buffer" in a protocol for making chemically competent E.coli - (reply: 3)
  4. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
  5. Identifying unknown e. coli - (reply: 3)
  6. Affordable source of LB broth for e.coli storage? - (reply: 8)
  7. Proper source of ampicillin for e.coli growth medium? - (reply: 2)
  8. Antibody against E.coli lysate - (reply: 1)
  9. Efficient lysis of E.coli cells - (reply: 4)
  10. Differences between DH5a strain of E. Coli and BL21 strain of E.Coli - (reply: 2)
  11. Expression of plant protein in Ecoli - (reply: 1)
  12. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
  13. E coli ST131 - (reply: 2)
  14. Transformation E.Coli help - (reply: 2)
  15. Collagen binding protein expression E. coli His-tag - (reply: 1)
  16. TOP10F Chemically Competent E. coli - (reply: 1)
  17. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
  18. Can't isolate plasmid from cloned E.coli - (reply: 4)
  19. Screen for F plasmid in e. coli - (reply: 1)
  20. vector propagation in E coli - (reply: 1)
  21. E coli culture cannot grow overnight. - (reply: 11)
  22. genotype of e coli strain for protein expression - (reply: 2)
  23. Identification of L.Mono and E.coli - (reply: 4)
  24. Fluoroquinolone resistant ecoli - (reply: 1)
  25. E.coli culture in -4c for 2 hours means it will affect or not? what happened in - (reply: 3)
  26. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  27. Expression in different E.coli strains - (reply: 3)
  28. Maintaining E. Coli (ATCC 25922) - (reply: 5)
  29. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
  30. E coli. and Staph identification problem - (reply: 1)
  31. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
  32. E. coli culture - (reply: 1)
  33. E coli, bacterial strain problem questions - (reply: 2)
  34. Phosphorylation of recombinant proteins in E. coli - (reply: 1)
  35. production of phosphorylated proteins in E.coli host system - (reply: 1)
  36. Selection of E.coli competent cells - (reply: 2)
  37. E. coli (ATCC 25922) growth in Minimal Medium - (reply: 5)
  38. Cloning cDNA construct into E.Coli. - (reply: 4)
  39. Grow multiple Ecoli colonies in a single liquid media tube - (reply: 4)
  40. Plasmid stability in Ecoli during storage - (reply: 3)
  41. Problems in expression of target protein in E. coli - (reply: 3)
  42. reference gene for qPCR in E.coli - (reply: 2)
  43. How to select competent E. coli cells? - (reply: 7)
  44. heterologous gene integration into e.coli genome - (reply: 1)
  45. Very small peptide expression in E. coli - (reply: 2)
  46. Continuous growth of E. coli on AMP LB plate - (reply: 2)
  47. Protein expression in e.coli - (reply: 5)
  48. PTM in E. coli - (reply: 1)
  49. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  50. siRNA in E.Coli - (reply: 2)
  51. Protein Extraction: Extremely Sticky e coli lysate - (reply: 1)
  52. Problems with Ecoli glycerol stock - (reply: 6)
  53. Unable to clone in E. coli cells - (reply: 3)
  54. Cleaning/eliminating E.coli adsorbed onto substrates - (reply: 1)
  55. SURE E. coli competent cells - (reply: 1)
  56. E. coli growing on plates and bot not in LB medium - (reply: 10)
  57. Dam-/+ e. coli strains - (reply: 6)
  58. E. coli strain for conjugation - (reply: 1)
  59. pkD46 vector growth in E.coli problem - (reply: 8)
  60. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  61. Polymerase for E. coli screenings - (reply: 5)
  62. Unknown contaminant in Ecoli culture - (reply: 4)
  63. Would E. Coli cells be stable at room temp, if in media with glycerol? - (reply: 1)
  64. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
  65. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  66. How to insert genes into chromosome of E. coli - (reply: 1)
  67. e coli vector and host selection - (reply: 1)
  68. E.coli grow on neg. control - (reply: 1)
  69. Co-expression of 2 plasmids in E.coli - (reply: 1)
  70. Ecoli bacteria storage - (reply: 3)
  71. transporting E coli on filter paper - (reply: 5)
  72. Problem with Transforming E. coli DH10Bac - (reply: 8)
  73. Plasmid vector for yeast and E. coli - (reply: 2)
  74. E. coli cells refuse to grow! - (reply: 2)
  75. blast bacterial genomes like e.coli - (reply: 1)
  76. What OD600 is suitable for assaying Ecoli viability - (reply: 9)
  77. ask for a strain for the suicide vector called E. coli S17-1 pir - (reply: 1)
  78. What do you expect to see in E. coli digested with EcoRI? - (reply: 8)
  79. Fermentation with E.coli XL10gold - (reply: 9)
  80. gene expression in E coli with PET21a vector - (reply: 1)
  81. co-expression of 2 plasmids in E.coli - (reply: 3)
  82. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
  83. protocol for making e.coli competent cells under low temperature - (reply: 1)
  84. Any competent or certain E Coli for mutagenesis transformation? - (reply: 1)
  85. No activity of fungi protein in E.coli - (reply: 15)
  86. Dropped e.coli (BL21) culture on clothes - (reply: 3)
  87. e.coli growth curve in microtiter plate - (reply: 1)
  88. protein expression level and OD600 in E.coli - (reply: 1)
  89. Strange E.coli growth - (reply: 3)
  90. stop codon readthrough in E.coli - (reply: 8)
  91. How to improve protein solubility in E. coli? - (reply: 11)
  92. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
  93. Upscaling protein expression in E.coli - (reply: 2)
  94. E coli K 12 - (reply: 1)
  95. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  96. E.coli cells lyse in minimal media - (reply: 3)
  97. e coli plate left too long at 37C - very small clones appeared (reply: 4)
  98. How much slower does E coli grow in M9 vs LB? - (reply: 2)
  99. Growth conditions for E. coli DB3.1 cells - (reply: 1)
  100. complementing a double knockout in E. coli - (reply: 1)
  101. Chemical competent E.coli always from single colony ? - (reply: 2)
  102. Expressing human protein in E.Coli - (reply: 12)
  103. Detergent-based lysis buffer reciipe for E coli - (reply: 6)
  104. growth of E.coli in minimal media(m9) is very less - growth of E.coli in minimal media(m9) is very less (reply: 2)
  105. E.Coli glycerol stock - (reply: 1)
  106. Cheap procedure for gene deletion in E coli? - (reply: 2)
  107. recombinant ecoli in log phase overnight media suggestion - (reply: 2)
  108. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  109. E coli CFUs - 48h incubation - silly Q! (reply: 2)
  110. Alcohol effect on genetic manipulated E.coli. - (reply: 1)
  111. Differentiating Ecoli from Agrobacteria quickly. - Noob mistake, I am paying for now. (reply: 2)
  112. E.coli suddenly death?? - (reply: 6)
  113. E.Coli growth at 4 degrees? - (reply: 8)
  114. Inclusion bodies in e.coli - (reply: 1)
  115. lysis of e.coli by sonication - (reply: 2)
  116. E. coli not growing in liquid cultures :( - (reply: 4)
  117. E.coli expression - (reply: 5)
  118. E coli growth at 30 C - (reply: 2)
  119. E coli lysis by lysozyme - (reply: 2)
  120. Pellet Formation - pellet formation for different concentrations of e.coli (reply: 1)
  121. e coli strain selection for protein expression - (reply: 2)
  122. e.coli contamination - (reply: 2)
  123. do e.coli cells get ruptured during high spin - (reply: 1)
  124. E. Coli culture in microplate - (reply: 5)
  125. functionality of yeast promoter in E. coli - (reply: 1)
  126. Good antibody for E. coli surface marker - phagocytosis assay (reply: 2)
  127. E coli transformation - (reply: 8)
  128. E. coli strain TB1 vs JM83 - Question about the hsdR mutation in TB1 (reply: 1)
  129. Incompatible restriction sites - Will E.coli ligate for me? (reply: 2)
  130. How to ask the author for an E. coli strains used in his paper? - (reply: 6)
  131. ccdB survival E. coli transformation - (reply: 2)
  132. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
  133. cempetent e.coli and transformation - (reply: 2)
  134. 4 reasons why E.coli is selected as indicator of water potability? - *I've tried few* (reply: 3)
  135. prepare sample protein for 2d from E.coli - (reply: 2)
  136. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  137. Picking strain for e coli competent cells - (reply: 6)
  138. Removal of Methionine in E.coli - (reply: 1)
  139. E.Coli expressing GFP - (reply: 4)
  140. Amount of inoculum? - for protein expression in E.coli (reply: 3)
  141. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  142. Best way to resuspend E. coli? - (reply: 7)
  143. look for E.coli DB3.1 bacteria - (reply: 2)
  144. Plasmid instability in E.coli BL21? - (reply: 3)
  145. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  146. Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) - (reply: 4)
  147. Phage removal of E.coli - (reply: 8)
  148. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  149. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
  150. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  151. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  152. freezing of E.coli culture prior DNA prep - (reply: 5)
  153. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
  154. Yellow E. coli colonies on MACCONKEY AGAR - (reply: 2)
  155. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
  156. Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
  157. Mammalian protein expression in E. coli - (reply: 1)
  158. Bacterial conjugation - between E. coli and Salmonella Typhimurium (reply: 1)
  159. E. coli Strains - (reply: 4)
  160. Multiple colony types from streaking 1 E.coli colony - (reply: 5)
  161. What's the best temperature for killing E.coli? - (reply: 4)
  162. making competent E coli DH5a cells - (reply: 6)
  163. E. coli colonies didn't grow up in liquid culture. -HELP- - (reply: 9)