E coli. and Staph identification problem - (Nov/13/2013 )
I have been given e coli, staphylococcus aureus and a selection of agars (see below) and I have to come up with an order in which I would culture them to start determining that the bacteria are in fact e coli and staphylococcus aureus as if I had no idea.
The agars are,
- Blood agar (incubate at 37oC overnight)
- Baird Parker agar (incubate at 37oC overnight)
- Slanetz and Bartley agar (incubate at 37oC overnight)
- CLED agar (incubate at 37oC overnight)
- Mueller Hinton agar (incubate at 37oC overnight)
- Sabouraud agar (incubate at 25oC for 3 days)
- TSC agar (incubate anaerobically at 37oC overnight)
- Nutrient broth (incubate at 37oC overnight)
I am able to use the oxoid to determine what each of these do however, I have no idea how I'd use them to determine e coli or staph if I didn't know what they were from the beginning,
Any help would be massively appreciated!!!
So what do the different media do? Are some differential and some selective (or both?)