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121. Setting the baseline / threshold in Opticon Monitor 3.1 - (reply: 1)
122. Internal Standards for RT-pCR - (reply: 11)
123. real time pcr newbie - (reply: 3)
124. How to get perfect amplification peaks - Share my long QPCR troubleshooting experience (reply: 1)
125. Amplification Effeciency - solve the problem with E value (reply: 2)
126. TaqMan probes specificity - I need to amplify and quantify specifically homologous genes (reply: 4)
127. Crazy dissociation curve, please help ! - Strange peaks in dissociation curve (reply: 6)
128. geNorm problem - VNF graph empty (reply: 2)
129. Reverse transcriptation for qPCR with some specific primers. Should I use primer - (reply: 2)
130. Calculating Relative Expression - how should I analyze my data????? (reply: 3)
131. Quantitation of non-polyA mRNA - (reply: 3)
132. What is the optimum region of the curve ? - (reply: 8)
133. Removing genomic DNA contamination -- Ambion Turbo DNase or Qiagen RNase-free DN - (reply: 4)
134. how to optimize primer and cDNA concentration for real-time - (reply: 2)
135. Help -designing primers for real time RT-PCR - (reply: 1)
136. Using real-time to differentiate between SNPs - (reply: 5)
137. how to calculate expression when control dont express - (reply: 3)
138. Bad primer efficiency during multiplex qPCR - (reply: 1)
139. Measuring cDNA concentration - (reply: 2)
140. re-pcr (2nd) different with pcr (1st) - (reply: 5)
141. Inefficient RT-PCR, ┬┐bad cDNA? - don't know why (reply: 1)
142. diluition 1:2 for standard curve - (reply: 1)
143. Great signal but nothing on gel - (reply: 4)
144. Problems with amplification curve - (reply: 5)
145. calculation of qRT-PCR - to Roche LC480 users (reply: 1)
146. Optimising Taqman probe - PCR is successful using SYBR but not with hydrolysis probe (reply: 3)
147. different transcription from 5' UTR than 3' UTR primers - (reply: 8)
148. What Ct value is accepted as expression? - qRT-PCR Ct values (reply: 3)
149. recommended primers for caspase 8 & 9 - human (reply: 4)
150. qRT-PCR first timer, controls? - (reply: 9)

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