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691. Self-made qRT-PCR enzyme-/mastermix? - (reply: 8)
692. RT issues and efficiency of cDNA synthesis - qRT-PCR (reply: 1)
693. Real time mastermix Storage condition - The temperature for storage (reply: 1)
694. EMERGENCY: ovine GAPDH NTC contamination - (reply: 7)
695. TaqMan probe - (reply: 4)
696. agarose-gel artefact - (reply: 1)
697. Volume of Real time PCR mastermix and reaction - (reply: 4)
698. Decreasing Amplification Curve for Standards - (reply: 2)
699. Home-made Sybr green mix for real-time PCR - (reply: 1)
700. Problem with calculation using comparative method - When housekeeping gene is expressed less than sample (reply: 1)
701. Reverse Transcription question - ssproduct or dsproduct (reply: 3)
702. Plasmid DNA purification - Lithium chlorid (reply: 1)
703. Why not optimise on the real time machine? - (reply: 5)
704. 16S rRNA primers for E. coli - (reply: 2)
705. Should I dilute cDNA for real-time PCR? - (reply: 3)
706. Real-time PCR problems - RT_PCR (reply: 7)
707. 1-step vs. 2-step Real Time PCR - pros and cons (reply: 4)
708. Starting real time rt-pcr - working with rna virus (reply: 7)
709. LinReg calculations - Does anybody have experience using LinReg with Light Cycler data? (reply: 2)
710. strange contamination in real-time PCR in negative control? - (reply: 1)
711. quantity of RNA to use in RT reaction - (reply: 3)
712. Using the (cheaper) Roche SYBR plate mastermix in capillaries... - Ever tried this? (reply: 1)
713. Homemade RealTime Syb-GreenI-Mix - (reply: 23)
714. SYBR Green PCR Master Mix storage - (reply: 3)
715. SYBR Green I - (reply: 7)
716. Absolute quantification method? - (reply: 3)
717. How to normalize with 2 housekeeping genes? - (reply: 4)
718. Problem with QPCR - control gene does not amplify - (reply: 2)
719. qPCR for HKG and traditional PCR for GOI - (reply: 1)
720. Smeary bands in real-time RT-PCR - (reply: 7)
692. RT issues and efficiency of cDNA synthesis - qRT-PCR (reply: 1)
693. Real time mastermix Storage condition - The temperature for storage (reply: 1)
694. EMERGENCY: ovine GAPDH NTC contamination - (reply: 7)
695. TaqMan probe - (reply: 4)
696. agarose-gel artefact - (reply: 1)
697. Volume of Real time PCR mastermix and reaction - (reply: 4)
698. Decreasing Amplification Curve for Standards - (reply: 2)
699. Home-made Sybr green mix for real-time PCR - (reply: 1)
700. Problem with calculation using comparative method - When housekeeping gene is expressed less than sample (reply: 1)
701. Reverse Transcription question - ssproduct or dsproduct (reply: 3)
702. Plasmid DNA purification - Lithium chlorid (reply: 1)
703. Why not optimise on the real time machine? - (reply: 5)
704. 16S rRNA primers for E. coli - (reply: 2)
705. Should I dilute cDNA for real-time PCR? - (reply: 3)
706. Real-time PCR problems - RT_PCR (reply: 7)
707. 1-step vs. 2-step Real Time PCR - pros and cons (reply: 4)
708. Starting real time rt-pcr - working with rna virus (reply: 7)
709. LinReg calculations - Does anybody have experience using LinReg with Light Cycler data? (reply: 2)
710. strange contamination in real-time PCR in negative control? - (reply: 1)
711. quantity of RNA to use in RT reaction - (reply: 3)
712. Using the (cheaper) Roche SYBR plate mastermix in capillaries... - Ever tried this? (reply: 1)
713. Homemade RealTime Syb-GreenI-Mix - (reply: 23)
714. SYBR Green PCR Master Mix storage - (reply: 3)
715. SYBR Green I - (reply: 7)
716. Absolute quantification method? - (reply: 3)
717. How to normalize with 2 housekeeping genes? - (reply: 4)
718. Problem with QPCR - control gene does not amplify - (reply: 2)
719. qPCR for HKG and traditional PCR for GOI - (reply: 1)
720. Smeary bands in real-time RT-PCR - (reply: 7)