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451. Sybergreen vs taqman? - Comparable? (reply: 1)
452. what are some general guidelines for manipulating Taq? - (reply: 2)
453. 18S Melt curve - sorry for the cross post.. this forum gets more traffic. (reply: 1)
454. Validating amplification efficiencies of the target and the housekeeping genes - (reply: 1)
455. Help with standard curve Ct values. - (reply: 7)
456. optimization of cDNA template for real time PCR array - (reply: 2)
457. normalization between 4 different run - (reply: 3)
458. qPCR vs RNA protection assay - (reply: 1)
459. Help of Q-PCR - (reply: 1)
460. REST-MCS data entry problems - (reply: 3)
461. cytokines primers - (reply: 1)
462. which method for relative quantitation? - (reply: 4)
463. Can you use Picogreen in a QPCR instrument? - (reply: 1)
464. amplicon size... when is too small too small? - (reply: 1)
465. Real Time PCR : Bacterial gene quantification, can it be done? - (reply: 2)
466. plasmids to quantify copies of virus - (reply: 4)
467. standards for copy number - please help (reply: 3)
468. Overloading a PCR? - Is this possible? (reply: 3)
469. different primer efficiency using cDNA and plasmid/PCR product - (reply: 4)
470. Efficiency greater than 100% using taqman probes? - (reply: 4)
471. Endogenous control validation - (reply: 1)
472. concentration of primers - (reply: 5)
473. RNA extraction from mouse splenocytes in 96 well plate - (reply: 1)
474. Agrobacterium tumefaciens 33970 mediated transformation of medicinal plant - Agrobacterium tumefaciens 33970 (reply: 8)
475. A strange phenomenon using differen reagents - (reply: 2)
476. housekeeping gene for lymphocyte - (reply: 1)
477. Problem - same ct values in my RT positive and negative samples - (reply: 3)
478. Two dissociation curve peaks in my standard curve - (reply: 1)
479. Taq Polymerase storage conditions - (reply: 1)
480. Primers & probes of Applied Biosystems for real time - (reply: 2)

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