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661. need helps on probe design--human ip10 primers and probe. - (reply: 2)
662. qPCR-growth curve - (reply: 2)
663. RT PCR on ALM patients - help for designing primers for detect PML RAR (reply: 2)
664. Multiple unknown amplification products - (reply: 2)
665. Primer design with incomplete sequence - (reply: 2)
666. Endogenous controls (housekeeping) for immunologists - (reply: 2)
667. HELP! confused with validation of efficiencies - (reply: 2)
668. generation of gDNA Std curves in qPCR - ABI 7300 qPCR (reply: 1)
669. Determine slope values for efficiency quantification using ABI 7500 - (reply: 8)
670. RT-PCR machine/setup problems - (reply: 2)
671. Melt curve analysis - (reply: 4)
672. Cheaper SYBR green mix? - (reply: 5)
673. Minimum amount of RNA for real-time PCR - (reply: 4)
674. Primer dimers - (reply: 1)
675. is cDNA concentration 200 ng/ul too high for qRT-PCR - (reply: 17)
676. Help. Real- time against nested - I need to run a real time experiment (reply: 1)
677. Better efficiency but bad melt curve - (reply: 1)
678. genomic dna in real-time pcr - (reply: 3)
679. More about PCR dimers! - (reply: 1)
680. HLA typing using qPCR - looking for references (reply: 2)
681. dissociation curve analysis - (reply: 2)
682. RNA extraction from small number of cells - (reply: 3)
683. Linear Regression analysis - need help interpreting results (reply: 1)
684. How reliable is 18S RNA as reference - (reply: 4)
685. design of standard-curves with low variance at very low copy numbers - (reply: 3)
686. quantifying cDNA for Real Time PCR - (reply: 3)
687. What should I do for more samples need to be tested by real-time PCR? - (reply: 1)
688. RNA integrity - (reply: 4)
689. Melt Curve Analysis - (reply: 2)
690. Not enough cDNA for qRT-PCR - how to increase cDNA yield or save cDNA? - (reply: 12)

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