geNorm problem - VNF graph empty (Jun/16/2008 )

hi everyone,

i have tested a 4 HSG in 2 samples in duplicate. Genorm was applied to
this data and it presented HPRT and B2M as most stable genes.

But why is the VNF chart empty? [attachment=4836:Results_geNorm.pdf]
the suitable reference gene-pair was calculated, so i'm wondering why the second chart is empty? my input data were delta-Ct values (efficiency corrected).

thank you for your help!

-strangefruit-

some time ago i had exactly the same problem.

I replaced the commas in my input file against points e.g. "1.0" instead of "1,0" and then it worked.

-Ned Land-

QUOTE (Ned Land @ Jun 16 2008, 07:57 PM)

some time ago i had exactly the same problem.

I replaced the commas in my input file against points e.g. "1.0" instead of "1,0" and then it worked.

thank you ned land,

INPUT FILE.. that's it! i was using the manual input option (with points as separator). with an input file everything works fine.

-strangefruit-