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241. Does ABI's Power SYBR Green need vortex befor used? - Vortex is necessary? (reply: 2)
242. SYBR green concentration for qPCR - (reply: 2)
243. Primers from RT-PCR database - RT-PCRdatabase primers giving primer dimers (reply: 1)
244. PCR efficiency for qRT-PCR analysis - PCR efficiency for qRT-PCR analysis (reply: 4)
245. qrt-pcr on bacterial transcripts - (reply: 1)
246. Genorm - Validation of housekeeping genes - (reply: 1)
247. Melting Temperature of Oligos - (reply: 1)
248. gradient PCR - PCR (reply: 4)
249. Real time PCR - RT-PCR vs Western blot (reply: 8)
250. real time PCR data analysis - method I have never heard of before (reply: 3)
251. Cleanign up RNA isolation - (reply: 3)
252. Is fluorochrome use to tag with a probe are light sensitive? - (reply: 1)
253. Can I run q RT real-time PCR based on cDNA amount? - (reply: 1)
254. Which program should I use - (reply: 1)
255. Problems with SYBR green assay - (reply: 4)
256. changing the syber green reagent - (reply: 1)
257. qPCR on low to non-existant template - (reply: 4)
258. elp with qPCR standard curve - (reply: 5)
259. SYBR Real time PCR - peak with shoulder - what to do? (reply: 9)
260. HELP with qPCR results - (reply: 3)
261. QPCR primer Pairs - (reply: 1)
262. Real Time gDNA allelic discrimination - help needed - (reply: 3)
263. How to design relative quantification study? - (reply: 2)
264. New to rt pcr real time - guidelines and advice (reply: 3)
265. realtime from different starting tissue masses - (reply: 1)
266. Identifying Long terminal repeats (LTRs) - (reply: 1)
267. qPCR Exp. Design - Reruning Exp. Control Samples - (reply: 3)
268. How to know tissue specific gene expression by real time PCR - (reply: 3)
269. sequence retrieval for primer design - (reply: 2)
270. Statistics: real-time RT-PCR - (reply: 4)

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