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631. ASAP: ONE STEP Real time RT PCR kit for TWO step RT-PCR method? - (reply: 1)
632. qPCR detection for single nucleotide substitution - (reply: 5)
633. do plasmids amplify more efficiently than genomic DNA? - (reply: 3)
634. bias optimalization - (reply: 1)
635. Question about Realtime PCR primer concentration.... - (reply: 5)
636. plasmid amplification - exons and primers (reply: 1)
637. plasmid DNA dilution for absolute quantitation - (reply: 2)
638. Efficiency>2! What does this mean? - (reply: 2)
639. Reverse Transcription with oligo dT or hexamer primers? - What do you prefer and why? (reply: 3)
640. Has anyone testet PrimerBank? - (reply: 12)
641. new to real time PCR- where to start? - help me (reply: 5)
642. PCR dry mixture - (reply: 3)
643. Use PCR products to determine efficiency of amplification? - (reply: 7)
644. Strange results with beta actin melt curve - Please Help... (reply: 3)
645. standard curve construction/input - specifically on BioRad iCycler (reply: 5)
646. Q-PCR questions: Primer design and melting curve - (reply: 13)
647. how to reconstitute water in this lyophillized oligodT? - (reply: 2)
648. Reference Gene - How close is close? - (reply: 4)
649. General idea about RT-PCR - RT-PCR (reply: 2)
650. taqman assays and linreg - (reply: 1)
651. PCR - free primer designation program (reply: 2)
652. Best product size for real-time RT-PCR - (reply: 4)
653. house keeping gene in skeletal muscle - (reply: 2)
654. Statistical evaluations for qRT-PCR - The comp. delta-delta CT method (reply: 5)
655. Which housekeeping genes for qRT-PCR control? - (reply: 17)
656. REST software/Pfaffl Analysis - (reply: 2)
657. Replication of qRT-PCR experiments - (reply: 5)
658. need helps on probe design--human ip10 primers and probe. - (reply: 2)
659. qPCR-growth curve - (reply: 2)
660. RT PCR on ALM patients - help for designing primers for detect PML RAR (reply: 2)

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