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181. TaqMan single v duplexing - (reply: 5)
182. Problems with cDNA - How long can I keep cDNA on 37°C? (reply: 3)
183. Housekeeping gene not affected by HGF treatment - (reply: 2)
184. Realtime PCR-negative control and dissociation curve! - (reply: 2)
185. primer design from mRNA - (reply: 12)
186. analysing real Time data - Induction Ver percentage (reply: 1)
187. paper request - (reply: 2)
188. standard curves sticking together - (reply: 5)
189. standard curve method for relative qPCR quantitation - (reply: 2)
190. q-RT-PCR on ds-cDNA? - (reply: 1)
191. How much cDNA from RNA toata?! - (reply: 2)
192. RNA extraction from laser capture microdissections - how to see low expressed genes from trought RT/qPCR on laser capture m (reply: 2)
193. Old samples - (reply: 2)
194. Primer concentration is quantitative Real-time PCR - (reply: 1)
195. Real time pcr in Rotor gene 6000- not getting positive results - (reply: 1)
196. choosing real-time PCR machine - (reply: 13)
197. Problems with Housekeeping/Endogenous Control - (reply: 4)
198. Lyticase and crude lyticase? - (reply: 1)
199. efficiency about 120% ?!? How? and WHY? - (reply: 2)
200. What Real Time-PCR data analysis method must I use? - (reply: 5)
201. Housekeeping genes for RT-PCR in soybean - (reply: 6)
202. iQ5 dynamic well factors for TaqMan probes - (reply: 5)
203. Real-Time PCR and Housekeeping gene - (reply: 9)
204. Can do reverse transcription by gene-specific primers? - (reply: 3)
205. TaqMan - 18S curve not sigmoidal, replicates are different - (reply: 4)
206. Taqman PCR master mix - (reply: 4)
207. Primer design (long transcripts) - (reply: 2)
208. Primers for Real time PCR - Really need some advice (reply: 1)
209. Standard curve needed? - (reply: 1)
210. Cell lysis buffer for real time RT-PCR - Request for cell lysis buffer recipe or relevant scientific paper (reply: 1)

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