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541. RT-PCR primer design - (reply: 1)
542. Primers work for regulary PCR but not real-time PCR - why? (reply: 8)
543. Where is my spike? - (reply: 2)
544. Real Time Contamination/Background Issues - (reply: 1)
545. standard curve and differentiation asays - Real Time PCR (reply: 2)
546. allele-specific amplification - (reply: 1)
547. Can you correct my abstract in English, please.. - (reply: 6)
548. qPCR Analysis - Standard Error of Individual dCT vs Group average expression (reply: 4)
549. Optical Adhesive Covers - bubbles (reply: 2)
550. Standard Curve Issues with ABI 7500 qPCR - (reply: 4)
551. change in expression of House keeping gene - change in expression of House keeping gene (reply: 2)
552. SYBR green problem.. - real time PCR SYBR (reply: 2)
553. primer dimer in pcr product - cloning for a gene. analyzing pcr product shows primer dimer (reply: 8)
554. relative quantification - (reply: 3)
555. relative quantitation - validation experiment - (reply: 1)
556. evaporation problem - (reply: 1)
557. what is fold change - (reply: 6)
558. Pre-Preparation of qPCR plates - Pros and Cons (reply: 4)
559. RT-PCR control for Vitis vinifera - (reply: 2)
560. Housekeeping gene always on the same plate as samples? - (reply: 1)
561. syber green with real time PCR - (reply: 17)
562. Inter-assay positional variance - Ct values seem to be dependent upon position of wells (reply: 4)
563. Re-amplify PCR product by realtime PCR? - (reply: 13)
564. genotyping SNP assays - (reply: 2)
565. Bands appear poor/not at all and do not appear consistantly & primer bands a - (reply: 2)
566. housekeeping gene in real-time PCR - (reply: 2)
567. Problem with DNase digestion of RNA - (reply: 6)
568. look at my efficiency! It sucks! - (reply: 1)
569. Trouble with real-time PCR standard curve - (reply: 4)
570. no RT controls - can I run on separate plate and for just one primer set? (reply: 1)

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