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Virus Related Discussions
  1. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
  2. Viral RNA in total RNA eluate for transfection - (reply: 1)
  3. nested PCR for low viral load- HBV patient sample - (reply: 2)
  4. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
  5. DNA virus purification from agarose failed! - (reply: 7)
  6. NGS of bi-partite viral genome - (reply: 2)
  7. Do I need to have a biological replicate for sequencing Virus - (reply: 3)
  8. How to inactivate H1N1 virus in Cell culture supernatant ? - (reply: 3)
  9. Virus sample - (reply: 3)
  10. Looking for Clontech Adeno-X-AcGFP1 control marker virus (#632504) - (reply: 6)
  11. How to design a coculture system with transwell insert for influenza virus studi - (reply: 2)
  12. Influenza virus - (reply: 3)
  13. effect of substance on viral infection - (reply: 4)
  14. DNA contamination from adenovirus afted DNase treatment - (reply: 2)
  15. Protein repeatedly does not express from pMX retroviral vector-advice needed des - (reply: 10)
  16. Viral contamination in DNA purification columns - (reply: 1)
  17. Adshuttle vectors and adenoviruses - (reply: 1)
  18. What is Role of HA in the biology of influenza virus and how this protein influe - (reply: 1)
  19. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
  20. Determining viral titer - (reply: 4)
  21. Will inactive virus particles make rabbit produce antibody? - (reply: 2)
  22. question about viral infection - (reply: 4)
  23. shRNA interference using Lenti virus vector - (reply: 1)
  24. Adenovirus infection, MOI and Transduction Efficiency - (reply: 1)
  25. Problem amplifying viral gene - (reply: 5)
  26. virus and cell surface receptor interaction - (reply: 4)
  27. Viral RNA extraction/isolation methods from plant tissue - (reply: 4)
  28. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
  29. Adenovirus for protease inhibitor studies. - (reply: 1)
  30. 9CFR virus testing (on bovine serum) - (reply: 3)
  31. Large scale Viral RNA purification Kit - (reply: 3)
  32. Quantifying Viral mRNAs at Different Time Points? - (reply: 2)
  33. Viral genomic DNA isolation ? - (reply: 3)
  34. the virus particles purification methods - (reply: 7)
  35. Chicken Egg Virus Harvest (Yolk Problem) - (reply: 3)
  36. How to quantify virus by PCR - (reply: 12)
  37. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  38. Recovery of viral RNA - (reply: 3)
  39. unable to amplify an infectious retrovirus clone - (reply: 4)
  40. Use dialysis tube to concentrate virus - (reply: 8)
  41. What is titration of viral progeny? - (reply: 8)
  42. Increased transcriptional activity upon retroviral infection - (reply: 2)
  43. virus transduction vs plasmid transfection - (reply: 4)
  44. i need help with virus pcr - (reply: 2)
  45. transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
  46. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
  47. adenoviral cre or lentiviral cre for mouse primary cells? - (reply: 2)
  48. Infecting cells with virus - (reply: 1)
  49. Retrovirus production & media color (pH) - (reply: 3)
  50. Viral RNA Extraction from Tissue Culture Supernatant - (reply: 5)
  51. Retroviral work - (reply: 1)
  52. Retrovirus/Lentivirus compatibility - (reply: 1)
  53. orientation of promoter into viral construct - (reply: 1)
  54. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  55. Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
  56. Viral transfection to neurosphere in vitro - (reply: 1)
  57. Looking for an affordable source for adenovirus service - (reply: 2)
  58. viral entry - (reply: 9)
  59. infection with two retroviruses? - Delivery of two Genes in distinct virus constructs, does it work? (reply: 3)
  60. Viral contamination - (reply: 3)
  61. Viral beta actin - Viral genomics (reply: 2)
  62. Fixation of virus for TEM - (reply: 3)
  63. Is production of recombinant virus difficult? - anybody done before? (reply: 4)
  64. Virus with serum - viral transfection (reply: 1)
  65. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  66. Strand specific probe design for Sindbis virus - (reply: 4)
  67. Filter for viral transfection - filter 0.22 or 0.45 (reply: 4)
  68. Viral cDNA Library - (reply: 3)
  69. TRIPZ lentiviral production - problems with viral production (reply: 5)
  70. shRNA produce virus - (reply: 2)
  71. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
  72. Problem with Lentiviral production - I have problems getting good virus titer!! (reply: 6)
  73. What is the proper way to infect cell culture with virus? - (reply: 5)
  74. Cell cycle analysis after viral transduction - Shift of peak (reply: 1)
  75. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  76. Calculating MOI of viral vector - (reply: 1)
  77. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  78. virus with human gene to mouse cell - Does it work? (reply: 2)
  79. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  80. lenti virus transfected cell dies... - (reply: 4)
  81. Retroviral transduction of T cells - (reply: 4)
  82. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
  83. Tissue homogenization buffer to keep viral RNA intact - (reply: 6)
  84. Retrovirus Production - I am not getting any virus from my packaging cells! (reply: 4)
  85. Viral DNA extraction - (reply: 1)
  86. Retrovirus production - How to produce retrovirus with Transient transfection? (reply: 2)
  87. plaque assay polio virus - plaque assay polio virus (reply: 2)
  88. Does a viral gene have introns? - (reply: 4)
  89. Virus titer - (reply: 1)
  90. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  91. stable cell line - selection after retroviral infection (reply: 1)
  92. shipping retrovirus supernatant - (reply: 2)
  93. how to calculate viral titer? - (reply: 1)
  94. Please save me, have trouble to make adenovirus from 293A cells - troubleshooting adenovirus production (reply: 1)
  95. ELISA of Sindbis virus from media - is that possible? (reply: 2)
  96. extraction of viral rna from plant tissue - (reply: 1)
  97. How to estimate viral titre? - (reply: 1)
  98. Stable Mammalian Expression with Retrovirus - (reply: 2)
  99. Virus envelope - way to eliminate virus envelope (reply: 1)
  100. RCAS virus infection - (reply: 3)
  101. RCAS virus stability - (reply: 2)
  102. viral vector - retroviral vectors (reply: 1)
  103. Two virus method for transfection - (reply: 1)
  104. Cos7 Cells to produce retrovirus - (reply: 2)
  105. What retrovirus vetor can have 7.5kb insert - (reply: 1)
  106. retrovirus shRNA - (reply: 1)
  107. No retrovirus?! Please help! - (reply: 7)
  108. Viral genome sequencing - (reply: 10)
  109. Purification of virus particles - (reply: 2)
  110. How to do viral titre counts? - (reply: 2)
  111. biochemistry of H1N1 virus - (reply: 5)
  112. adeno virus infection - (reply: 5)
  113. retroviral concentration - (reply: 1)
  114. Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
  115. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  116. retroviral generation of stable cell line - cells just don't seem to be growing (reply: 3)
  117. Accuracy of virus counting methods - (reply: 2)
  118. Invitrogen's BLOCK-IT lentiviral kit - problems getting virus (reply: 5)
  119. How to concentrate lenti or retroviral particules - (reply: 3)
  120. Lenti viral Vector with GFP - (reply: 4)
  121. how to concentrate/purify virus without ultracentrifugation? - (reply: 5)
  122. low viral load in HIV positive sample - low viral load in HIV positive sample (reply: 1)
  123. Retroviral vector with luciferase - (reply: 1)
  124. Virus storage - (reply: 9)
  125. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  126. Chikungunya Virus Culture - Chikungunya Virus culture in mammalian cells form serum sample of huma (reply: 2)
  127. PEG adenovirus purification - enough pure for retinal injection in rat?? (reply: 5)
  128. What is the biggest insert for a viral vector? - (reply: 15)