Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Virus Related Discussions
  1. Sequencing virus from liver samples - (reply: 1)
  2. Virus vs protein purification - (reply: 3)
  3. How many NGS readings for a virus genome? - (reply: 6)
  4. What gene to insert in my anti-cancer virus? - (reply: 2)
  5. Viral Quantification - (reply: 1)
  6. Discuss a possible way to cure virus disease - (reply: 1)
  7. Lab technique to document implication of a virus in a disease? - (reply: 2)
  8. Inconsistent results with retroviral transduction - (reply: 1)
  9. How to fully identify the virus? - (reply: 1)
  10. Virus processing (filtration) of blood - How to? - (reply: 5)
  11. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
  12. Ebola Virus - (reply: 1)
  13. Viral RNA in total RNA eluate for transfection - (reply: 1)
  14. nested PCR for low viral load- HBV patient sample - (reply: 2)
  15. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
  16. DNA virus purification from agarose failed! - (reply: 7)
  17. NGS of bi-partite viral genome - (reply: 2)
  18. Do I need to have a biological replicate for sequencing Virus - (reply: 3)
  19. How to inactivate H1N1 virus in Cell culture supernatant ? - (reply: 3)
  20. Virus sample - (reply: 3)
  21. Looking for Clontech Adeno-X-AcGFP1 control marker virus (#632504) - (reply: 6)
  22. How to design a coculture system with transwell insert for influenza virus studi - (reply: 2)
  23. Influenza virus - (reply: 3)
  24. effect of substance on viral infection - (reply: 4)
  25. DNA contamination from adenovirus afted DNase treatment - (reply: 2)
  26. Protein repeatedly does not express from pMX retroviral vector-advice needed des - (reply: 10)
  27. Viral contamination in DNA purification columns - (reply: 1)
  28. Adshuttle vectors and adenoviruses - (reply: 1)
  29. What is Role of HA in the biology of influenza virus and how this protein influe - (reply: 1)
  30. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
  31. Determining viral titer - (reply: 4)
  32. Will inactive virus particles make rabbit produce antibody? - (reply: 2)
  33. question about viral infection - (reply: 4)
  34. shRNA interference using Lenti virus vector - (reply: 1)
  35. Adenovirus infection, MOI and Transduction Efficiency - (reply: 1)
  36. Problem amplifying viral gene - (reply: 5)
  37. virus and cell surface receptor interaction - (reply: 4)
  38. Viral RNA extraction/isolation methods from plant tissue - (reply: 4)
  39. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
  40. Adenovirus for protease inhibitor studies. - (reply: 1)
  41. 9CFR virus testing (on bovine serum) - (reply: 3)
  42. Large scale Viral RNA purification Kit - (reply: 3)
  43. Quantifying Viral mRNAs at Different Time Points? - (reply: 2)
  44. Viral genomic DNA isolation ? - (reply: 3)
  45. the virus particles purification methods - (reply: 7)
  46. Chicken Egg Virus Harvest (Yolk Problem) - (reply: 3)
  47. How to quantify virus by PCR - (reply: 12)
  48. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  49. Recovery of viral RNA - (reply: 3)
  50. unable to amplify an infectious retrovirus clone - (reply: 4)
  51. Use dialysis tube to concentrate virus - (reply: 8)
  52. What is titration of viral progeny? - (reply: 8)
  53. Increased transcriptional activity upon retroviral infection - (reply: 2)
  54. virus transduction vs plasmid transfection - (reply: 4)
  55. i need help with virus pcr - (reply: 2)
  56. transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
  57. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
  58. adenoviral cre or lentiviral cre for mouse primary cells? - (reply: 2)
  59. Infecting cells with virus - (reply: 1)
  60. Retrovirus production & media color (pH) - (reply: 3)
  61. Viral RNA Extraction from Tissue Culture Supernatant - (reply: 5)
  62. Retroviral work - (reply: 1)
  63. Retrovirus/Lentivirus compatibility - (reply: 1)
  64. orientation of promoter into viral construct - (reply: 1)
  65. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  66. Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
  67. Viral transfection to neurosphere in vitro - (reply: 1)
  68. Looking for an affordable source for adenovirus service - (reply: 2)
  69. viral entry - (reply: 9)
  70. infection with two retroviruses? - Delivery of two Genes in distinct virus constructs, does it work? (reply: 3)
  71. Viral contamination - (reply: 3)
  72. Viral beta actin - Viral genomics (reply: 2)
  73. Fixation of virus for TEM - (reply: 3)
  74. Is production of recombinant virus difficult? - anybody done before? (reply: 4)
  75. Virus with serum - viral transfection (reply: 1)
  76. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  77. Strand specific probe design for Sindbis virus - (reply: 4)
  78. Filter for viral transfection - filter 0.22 or 0.45 (reply: 4)
  79. Viral cDNA Library - (reply: 3)
  80. TRIPZ lentiviral production - problems with viral production (reply: 5)
  81. shRNA produce virus - (reply: 2)
  82. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
  83. Problem with Lentiviral production - I have problems getting good virus titer!! (reply: 6)
  84. What is the proper way to infect cell culture with virus? - (reply: 5)
  85. Cell cycle analysis after viral transduction - Shift of peak (reply: 1)
  86. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  87. Calculating MOI of viral vector - (reply: 1)
  88. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  89. virus with human gene to mouse cell - Does it work? (reply: 2)
  90. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  91. lenti virus transfected cell dies... - (reply: 4)
  92. Retroviral transduction of T cells - (reply: 4)
  93. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
  94. Tissue homogenization buffer to keep viral RNA intact - (reply: 6)
  95. Retrovirus Production - I am not getting any virus from my packaging cells! (reply: 4)
  96. Viral DNA extraction - (reply: 1)
  97. Retrovirus production - How to produce retrovirus with Transient transfection? (reply: 2)
  98. plaque assay polio virus - plaque assay polio virus (reply: 2)
  99. Does a viral gene have introns? - (reply: 4)
  100. Virus titer - (reply: 1)
  101. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  102. stable cell line - selection after retroviral infection (reply: 1)
  103. shipping retrovirus supernatant - (reply: 2)
  104. how to calculate viral titer? - (reply: 1)
  105. Please save me, have trouble to make adenovirus from 293A cells - troubleshooting adenovirus production (reply: 1)
  106. ELISA of Sindbis virus from media - is that possible? (reply: 2)
  107. extraction of viral rna from plant tissue - (reply: 1)
  108. How to estimate viral titre? - (reply: 1)
  109. Stable Mammalian Expression with Retrovirus - (reply: 2)
  110. Virus envelope - way to eliminate virus envelope (reply: 1)
  111. RCAS virus infection - (reply: 3)
  112. RCAS virus stability - (reply: 2)
  113. viral vector - retroviral vectors (reply: 1)
  114. Two virus method for transfection - (reply: 1)
  115. Cos7 Cells to produce retrovirus - (reply: 2)
  116. What retrovirus vetor can have 7.5kb insert - (reply: 1)
  117. retrovirus shRNA - (reply: 1)
  118. No retrovirus?! Please help! - (reply: 7)
  119. Viral genome sequencing - (reply: 10)
  120. Purification of virus particles - (reply: 2)
  121. How to do viral titre counts? - (reply: 2)
  122. biochemistry of H1N1 virus - (reply: 5)
  123. adeno virus infection - (reply: 5)
  124. retroviral concentration - (reply: 1)
  125. Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
  126. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  127. retroviral generation of stable cell line - cells just don't seem to be growing (reply: 3)
  128. Accuracy of virus counting methods - (reply: 2)
  129. Invitrogen's BLOCK-IT lentiviral kit - problems getting virus (reply: 5)
  130. How to concentrate lenti or retroviral particules - (reply: 3)
  131. Lenti viral Vector with GFP - (reply: 4)
  132. how to concentrate/purify virus without ultracentrifugation? - (reply: 5)
  133. low viral load in HIV positive sample - low viral load in HIV positive sample (reply: 1)
  134. Retroviral vector with luciferase - (reply: 1)
  135. Virus storage - (reply: 9)
  136. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  137. Chikungunya Virus Culture - Chikungunya Virus culture in mammalian cells form serum sample of huma (reply: 2)
  138. PEG adenovirus purification - enough pure for retinal injection in rat?? (reply: 5)
  139. What is the biggest insert for a viral vector? - (reply: 15)