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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
361. Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin - (reply: 4)
362. novel microRNA prediction - (reply: 2)
363. pri-miR transcript - where does transcription start? (reply: 1)
364. siRNA electroporation - (reply: 1)
365. small RNA Northern - duration of transfer (reply: 1)
366. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
367. microRNAs - Extraction methods (reply: 3)
368. Is it possible to reuse electroporation cuvette to transfect siRNA - (reply: 4)
369. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
370. Problems with gene knockdown - No knockdown with validated shRNAs :( (reply: 6)
371. translation of cleaved miRNA targets - (reply: 3)
372. Silencer® GFP (eGFP) siRNA – AM4626 - (reply: 2)
373. Hammerhead ribozyme cleavage - (reply: 2)
374. siRNA - (reply: 2)
375. in vitro siRNA - wich is the best company for a siRNA for in vitro assay? (reply: 2)
376. prediction of novel microRNAs from deep sequencing data? - microRNA deep sequencing (reply: 1)
377. RNAi - (reply: 1)
378. pcDNA/pSUPER - (reply: 1)
379. ready made or customize miRNA microarray chip - (reply: 2)
380. shRNA stable lines - no stable reduction seen (reply: 3)
381. miRNA isolation from liquid samples - (reply: 18)
382. Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
383. annotated 3'utr? - (reply: 1)
384. Max. length for 3'UTR in luciferase assay? - (reply: 1)
385. target protector and site mutagenesis - how to make it? (reply: 6)
386. Complementation experiment - (reply: 4)
387. Why the fluoresence is dim or dispear in miRNA vector? - miRNA (reply: 3)
388. miRNA mimics - (reply: 2)
389. why using siRNA knock down,but get the mRNA products? - (reply: 3)
390. In vitro shRNA screening trouble - (reply: 4)

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