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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
301. about pre-miRNA cloned into lentivirus vectors - (reply: 4)
302. How to get pri-microRNA sequence of plants - (reply: 1)
303. shRNA negative control - (reply: 2)
304. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
305. One miRNA modulating expression of about 600 genes - Link to paper (reply: 1)
306. Cos7 Cells to produce retrovirus - (reply: 2)
307. miRNA gene UTRs? - (reply: 3)
308. Verified RNAi Sequences - (reply: 4)
309. Transfection Efficacy - FAM-labelled NT or Lamin? (reply: 2)
310. siRNA and plasmid cotransfection - (reply: 1)
311. rna from plasmid - (reply: 1)
312. shRNA stable line - need better reduction of target (reply: 8)
313. T cell transduction - (reply: 3)
314. retrovirus shRNA - (reply: 1)
315. design parameters for multiple siRNA expression in vector - (reply: 4)
316. Pronounciation of miRNA - (reply: 2)
317. miRNA TARGETS - (reply: 4)
318. 3'UTR constructs - any available commercially (reply: 1)
319. Concatemerization of miRNA... - (reply: 1)
320. Co-transfection of miRNA and vector expressing target sequence - (reply: 2)
321. How to overexpress star miRNA (miRNA*) from pre-miRNA construct - (reply: 4)
322. Who are the big names in miRNA research? - (reply: 5)
323. ShRNA vectors for other than mammalian systems - shRNA for S. pombe (reply: 6)
324. MiRna - How to screen the MiRNA Targets (reply: 2)
325. Is my primer going to work for qRT-PCR? - (reply: 6)
326. mature star miRNA - (reply: 2)
327. ? degenerate base codes, e.g., D = U, G, A - (reply: 1)
328. Can Oligofectamine be used in this way? - (reply: 1)
329. Inhibition of miRNA doesn't enhance its target expression - (reply: 4)
330. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 4)

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