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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
301. shRNA negative control - (reply: 2)
302. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
303. One miRNA modulating expression of about 600 genes - Link to paper (reply: 1)
304. Cos7 Cells to produce retrovirus - (reply: 2)
305. miRNA gene UTRs? - (reply: 3)
306. Verified RNAi Sequences - (reply: 4)
307. Transfection Efficacy - FAM-labelled NT or Lamin? (reply: 2)
308. siRNA and plasmid cotransfection - (reply: 1)
309. rna from plasmid - (reply: 1)
310. shRNA stable line - need better reduction of target (reply: 8)
311. T cell transduction - (reply: 3)
312. retrovirus shRNA - (reply: 1)
313. design parameters for multiple siRNA expression in vector - (reply: 4)
314. Pronounciation of miRNA - (reply: 2)
315. miRNA TARGETS - (reply: 4)
316. 3'UTR constructs - any available commercially (reply: 1)
317. Concatemerization of miRNA... - (reply: 1)
318. Co-transfection of miRNA and vector expressing target sequence - (reply: 2)
319. How to overexpress star miRNA (miRNA*) from pre-miRNA construct - (reply: 4)
320. Who are the big names in miRNA research? - (reply: 5)
321. ShRNA vectors for other than mammalian systems - shRNA for S. pombe (reply: 6)
322. MiRna - How to screen the MiRNA Targets (reply: 2)
323. Is my primer going to work for qRT-PCR? - (reply: 6)
324. mature star miRNA - (reply: 2)
325. ? degenerate base codes, e.g., D = U, G, A - (reply: 1)
326. Can Oligofectamine be used in this way? - (reply: 1)
327. Inhibition of miRNA doesn't enhance its target expression - (reply: 4)
328. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 4)
329. siRNA with low mRNA levels - (reply: 7)
330. shRNA plasmid - which company is good? (reply: 2)

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