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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
121. mir30-based shrna cloning-mismatch - (reply: 1)
122. New RNAi video from Nature - (reply: 1)
123. checking whther there is any miRNA in introns of a specific gene. - (reply: 1)
124. What to do Post-transfection of cells with siRNA? - (reply: 2)
125. miRNA in serum - (reply: 1)
126. Difference between <Antisense RNA> & <RNAi>? - (reply: 1)
127. How to predict targets from a novel miRNA - (reply: 1)
128. Knock down efficiency - (reply: 2)
129. Let-7 and miR-143 miRNA? - (reply: 1)
130. protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
131. Targets of mirna* - (reply: 1)
132. Which company to buy siRNA from? - (reply: 1)
133. How to predict targets of unkown miRNAs in Drosophila - (reply: 2)
134. shRNA rescue with site directed mutagenesis - oligo design - (reply: 4)
135. How to explain the difference between microarray results and qPCR data - (reply: 1)
136. placing siRNA seq into miRNA stem-loop - (reply: 4)
137. How many RNA I need for detecting plasma miR expression? - (reply: 1)
138. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
139. Totai RNA dilution - (reply: 8)
140. Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
141. problem with miRNA functional study - (reply: 1)
142. problem with siRNA transfection of HEK cells - (reply: 5)
143. Decreased siRNA performance at high concentrations: feasible? - (reply: 1)
144. Basic question: sc- vs. sc+ - (reply: 2)
145. 3'UTR sequence of a gene - (reply: 3)
146. how does the binding of miRNA at 3'UTR regulates the gene - (reply: 3)
147. Promoter activity of microRNA - (reply: 3)
148. renilla values are going down with firefly values after miRNA transfection - (reply: 1)
149. shRNA screening - (reply: 2)
150. Phenotypes with two different siRNA but no knockdown? - trouble in showing a knock-down (reply: 2)

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