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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
121. placing siRNA seq into miRNA stem-loop - (reply: 4)
122. How many RNA I need for detecting plasma miR expression? - (reply: 1)
123. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
124. Totai RNA dilution - (reply: 8)
125. Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
126. problem with miRNA functional study - (reply: 1)
127. problem with siRNA transfection of HEK cells - (reply: 5)
128. Decreased siRNA performance at high concentrations: feasible? - (reply: 1)
129. Basic question: sc- vs. sc+ - (reply: 2)
130. 3'UTR sequence of a gene - (reply: 3)
131. how does the binding of miRNA at 3'UTR regulates the gene - (reply: 3)
132. Promoter activity of microRNA - (reply: 3)
133. renilla values are going down with firefly values after miRNA transfection - (reply: 1)
134. shRNA screening - (reply: 2)
135. Phenotypes with two different siRNA but no knockdown? - trouble in showing a knock-down (reply: 2)
136. Which miRNA probe to choose? - (reply: 1)
137. Sigma esiRNA induces target gene expression - (reply: 1)
138. miRNA knockdown experiment controls - Very confused (reply: 2)
139. How to explain this luciferase result?! - (reply: 2)
140. Help on mIcroRNA expression levels - concentration needed (reply: 1)
141. siRNA optimization - (reply: 5)
142. designing mutant 3'UTR - (reply: 2)
143. problem with obtaining iRNA: AT rich sequences??? - (reply: 1)
144. miRNA from serum - (reply: 1)
145. siRNA transfection with lipofectamine 2000 - (reply: 1)
146. Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
147. Will transfected plasmids induce cytokine production? - (reply: 1)
148. miRNA knockdown approaches - (reply: 6)
149. siRNA screening and RNAse free - (reply: 1)
150. ShRNA transfection - (reply: 1)

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