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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
391. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
392. about the total RNA isolation (trizol methods) and further Dnase I - (reply: 3)
393. Qiagen miRNeasy kit - (reply: 2)
394. So confused with RNAi - (reply: 3)
395. Probe size for miRNA Northern blot? - (reply: 8)
396. Lenti viral Vector with GFP - (reply: 4)
397. inducible shRNA vector system - (reply: 7)
398. To Fizban - miRNA (reply: 13)
399. shRNA from SIGMA - (reply: 5)
400. Could I use transcriptomics data to look for pri-miRNA sequence - (reply: 2)
401. RNAi in Zebrafish - vector for zebrafish knock-down (reply: 4)
402. How many bp should be left at 5' and 3' flanking regions needed for miRN - miRNA (reply: 4)
403. siRNA concentration in divinding-growing cells - (reply: 2)
404. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
405. miRNA Northern Blot gel - (reply: 4)
406. How to design probes for microRNA Northern Blot - (reply: 4)
407. siRNA resistant gene - How many bp to change? (reply: 4)
408. siRNA cell function examples - (reply: 1)
409. shRNA transgenic mice - (reply: 1)
410. Cloning multiple miR30 based shRNAs into a Lenti - (reply: 2)
411. Good SiRNA Gene silencing - (reply: 1)
412. miRNA turnover rate - how long are endogenous miRs 'viable' (reply: 1)
413. ? definition of: thermodynamic stability at the 5' end - (reply: 2)
414. To Fizban: miRNA validation - (reply: 5)
415. cellular siRNA localization - (reply: 4)
416. Ncode miRNA RT & qPCR system - can mRNA be reverse transcribed in same RT reaction? (reply: 4)
417. Define Trasnsfection efficiency interms of siRNA experiment - Sirna uptake Vs transfection efficiency (reply: 12)
418. Amaxa siRNA - (reply: 3)
419. Dicer-KO - are lines available commercially (reply: 4)
420. workflow - start from microarray - (reply: 4)

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