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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
211. siRNA newb - How to go about starting an siRNA project (reply: 3)
212. lentivirus - possible to use 2 transfer vectors? (reply: 1)
213. problem in reporducing my result!!! - miRNA expression (reply: 1)
214. Same backbone different GFP intensity - (reply: 2)
215. Lentivirus-based Knockdown for miRNAs? - for hard-to-transfect cells (reply: 10)
216. How about Exiqon miRCURY miRNA PCR system? - (reply: 2)
217. Lentivirus toxicity - (reply: 3)
218. injection pri-mirna into Cytoplasm - (reply: 5)
219. Retrovirus Production - I am not getting any virus from my packaging cells! (reply: 4)
220. Help: protocols for shRNA knockdown effect on Ag-specific Treg differentiation - (reply: 1)
221. Weird effect of siRNA on protein in cells - (reply: 3)
222. How to overexpress miRs - (reply: 1)
223. siRNA to shRNA...trouble! - (reply: 1)
224. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
225. Silencing of a miRNA family - (reply: 1)
226. miRNA overexpression for target validation - (reply: 1)
227. nonreproducible knockdown efficiency with Plemir-micorRNA from Open Biosystem - (reply: 3)
228. How to Design a Negative Control siRNA - (reply: 1)
229. MiRNA detection and info - An research on MiRNA detection (reply: 5)
230. pre-miRNA (ambion) transfection troubleshooting - I am getting opposite results than expected (reply: 6)
231. QUESTION-CAPLE OF siRNAs sequences - (reply: 2)
232. transfecting cells with mature miRNA or precursor miRNAs? - (reply: 5)
233. microRNA functional classification - (reply: 1)
234. miRNAs and antibiotics - (reply: 1)
235. Retrovirus production - How to produce retrovirus with Transient transfection? (reply: 2)
236. Obtaining 3'UTR and perform microRNA target prediction - How to commence? (reply: 2)
237. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
238. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
239. how long will it takes to silence target gene after induction of shRNA - (reply: 2)
240. Protein shows KD but RNA level is higher - (reply: 4)

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