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91. problem with siRNA transfection of HEK cells - (reply: 5)
92. Decreased siRNA performance at high concentrations: feasible? - (reply: 1)
93. Basic question: sc- vs. sc+ - (reply: 2)
94. 3'UTR sequence of a gene - (reply: 3)
95. how does the binding of miRNA at 3'UTR regulates the gene - (reply: 3)
96. Promoter activity of microRNA - (reply: 3)
97. renilla values are going down with firefly values after miRNA transfection - (reply: 1)
98. shRNA screening - (reply: 2)
99. Phenotypes with two different siRNA but no knockdown? - trouble in showing a knock-down (reply: 2)
100. Which miRNA probe to choose? - (reply: 1)
101. Sigma esiRNA induces target gene expression - (reply: 1)
102. miRNA knockdown experiment controls - Very confused (reply: 2)
103. How to explain this luciferase result?! - (reply: 2)
104. Help on mIcroRNA expression levels - concentration needed (reply: 1)
105. siRNA optimization - (reply: 5)
106. designing mutant 3'UTR - (reply: 2)
107. problem with obtaining iRNA: AT rich sequences??? - (reply: 1)
108. miRNA from serum - (reply: 1)
109. siRNA transfection with lipofectamine 2000 - (reply: 1)
110. Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
111. Will transfected plasmids induce cytokine production? - (reply: 1)
112. miRNA knockdown approaches - (reply: 6)
113. siRNA screening and RNAse free - (reply: 1)
114. ShRNA transfection - (reply: 1)
115. miRNA/siRNA transfection doubts - (reply: 2)
116. Sequencing miRNA qRT-PCR Products - (reply: 5)
117. PCR of microRNA first strand cDNA - (reply: 1)
118. optimal size for 3'UTR cloning - miRNA and 3'UTR cloning (reply: 2)
119. How to express a rare microRNA - (reply: 2)
120. miRNA LNA - how can I designed a scramble ? (reply: 1)
92. Decreased siRNA performance at high concentrations: feasible? - (reply: 1)
93. Basic question: sc- vs. sc+ - (reply: 2)
94. 3'UTR sequence of a gene - (reply: 3)
95. how does the binding of miRNA at 3'UTR regulates the gene - (reply: 3)
96. Promoter activity of microRNA - (reply: 3)
97. renilla values are going down with firefly values after miRNA transfection - (reply: 1)
98. shRNA screening - (reply: 2)
99. Phenotypes with two different siRNA but no knockdown? - trouble in showing a knock-down (reply: 2)
100. Which miRNA probe to choose? - (reply: 1)
101. Sigma esiRNA induces target gene expression - (reply: 1)
102. miRNA knockdown experiment controls - Very confused (reply: 2)
103. How to explain this luciferase result?! - (reply: 2)
104. Help on mIcroRNA expression levels - concentration needed (reply: 1)
105. siRNA optimization - (reply: 5)
106. designing mutant 3'UTR - (reply: 2)
107. problem with obtaining iRNA: AT rich sequences??? - (reply: 1)
108. miRNA from serum - (reply: 1)
109. siRNA transfection with lipofectamine 2000 - (reply: 1)
110. Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
111. Will transfected plasmids induce cytokine production? - (reply: 1)
112. miRNA knockdown approaches - (reply: 6)
113. siRNA screening and RNAse free - (reply: 1)
114. ShRNA transfection - (reply: 1)
115. miRNA/siRNA transfection doubts - (reply: 2)
116. Sequencing miRNA qRT-PCR Products - (reply: 5)
117. PCR of microRNA first strand cDNA - (reply: 1)
118. optimal size for 3'UTR cloning - miRNA and 3'UTR cloning (reply: 2)
119. How to express a rare microRNA - (reply: 2)
120. miRNA LNA - how can I designed a scramble ? (reply: 1)