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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
91. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
92. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)
93. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
94. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)
95. validation of microRNA target gene - (reply: 6)
96. Can I use custom made synthetic miRNA for Overexpression? - (reply: 1)
97. Little knockdown but big effect on proliferation - (reply: 2)
98. Knockdown induce apoptosis - (reply: 5)
99. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
100. Converting shRNA sequence to siRNA - (reply: 1)
101. Can not reduce protein level - (reply: 1)
102. target mRNA/protein levels increase with siRNA - (reply: 2)
103. One siRNA sequence, many cell lines - (reply: 1)
104. miRNA from plasma_purity problem after RNA isolation - (reply: 1)
105. Substituting miRNA with ODNs for testing efficiency of dendrimers...? - (reply: 4)
106. RNAi in combination with adenoviral-mediated over-expression - (reply: 1)
107. microRNA exiqon individual assays - (reply: 1)
108. Oligo primers for shRNA construction - (reply: 1)
109. Antagomir design tool - (reply: 3)
110. Guide vs passenger strand sorting - (reply: 1)
111. How to explain this result? - (reply: 1)
112. Need an effective siRNA/ shRNA sequence for downregulating caveolin-1 - (reply: 2)
113. Higher mRNA level after RNAi? - (reply: 1)
114. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
115. question: RNAi for bacteria? - (reply: 3)
116. RNAi problem - Gene is knocked down, but not protein - (reply: 9)
117. Calculating siRNA Transfection Efficiency - (reply: 3)
118. miRNA extraction from cell-conditioned media? - (reply: 1)
119. si/shRNA sequences against eGFP - (reply: 1)
120. microRNA and 3' UTR - (reply: 2)

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