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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
31. miRNA-mediated competing endogenous RNAs (ceRNAs) networks - (reply: 4)
32. software for drawing small RNA molecules and nucleotides - (reply: 2)
33. miRNA knockdown in a mouse, tail vein injection (~50% KD) - (reply: 2)
34. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
35. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
36. Help with shRNAmiR30 based constructs (for a simple MD) - (reply: 3)
37. Question: How to differentiate siRNAs and mature miRNAs in plant by Northern blo - (reply: 3)
38. synthetic microRNA stability - (reply: 1)
39. siRNA detection......which strand shuld labelled - (reply: 2)
40. scrambeled miR sequence - (reply: 5)
41. miRNA overexpression troubleshooting - (reply: 2)
42. siRNA quantification using UV - (reply: 1)
43. overexpress mature microRNA by siRNA vector - (reply: 2)
44. MiRNA obtaining - (reply: 1)
45. Gene of interest targeting micrornas - (reply: 6)
46. Trouble with shRNA transfection - (reply: 2)
47. need help for trouble shooting the contamination in miR-X assay from serum - (reply: 1)
48. miRNA sequencing from FFPE tissue - (reply: 3)
49. which to use: miR or miRexpression vector - (reply: 1)
50. RNA journals - (reply: 4)
51. Detecting miRNA mimics by RT-PCR? - (reply: 4)
52. shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages - (reply: 1)
53. RNA alcohol/salt precipitation...low temperature - (reply: 1)
54. To small amount of cells used for shRNA transfection - (reply: 1)
55. shRNA interference using Lenti virus vector - (reply: 1)
56. Dual Luciferase Assay - (reply: 1)
57. Overexpressing miR - rescue by co-overexpressing its target - (reply: 1)
58. miRNA quantification - (reply: 3)
59. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
60. Gene knockdown expression levels shRNAmirs - (reply: 2)

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