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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
31. Question: How to differentiate siRNAs and mature miRNAs in plant by Northern blo - (reply: 3)
32. synthetic microRNA stability - (reply: 1)
33. siRNA detection......which strand shuld labelled - (reply: 2)
34. scrambeled miR sequence - (reply: 5)
35. miRNA overexpression troubleshooting - (reply: 2)
36. siRNA quantification using UV - (reply: 1)
37. overexpress mature microRNA by siRNA vector - (reply: 2)
38. MiRNA obtaining - (reply: 1)
39. Gene of interest targeting micrornas - (reply: 6)
40. Trouble with shRNA transfection - (reply: 2)
41. need help for trouble shooting the contamination in miR-X assay from serum - (reply: 1)
42. miRNA sequencing from FFPE tissue - (reply: 3)
43. which to use: miR or miRexpression vector - (reply: 1)
44. RNA journals - (reply: 4)
45. Detecting miRNA mimics by RT-PCR? - (reply: 4)
46. shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages - (reply: 1)
47. RNA alcohol/salt precipitation...low temperature - (reply: 1)
48. To small amount of cells used for shRNA transfection - (reply: 1)
49. shRNA interference using Lenti virus vector - (reply: 1)
50. Dual Luciferase Assay - (reply: 1)
51. Overexpressing miR - rescue by co-overexpressing its target - (reply: 1)
52. miRNA quantification - (reply: 3)
53. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
54. Gene knockdown expression levels shRNAmirs - (reply: 2)
55. siRNA in E.Coli - (reply: 2)
56. siRNA resuspension in RNase-free water - (reply: 1)
57. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
58. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
59. microRNA promoter based expression vector? - (reply: 1)
60. miRNA expression profiling - (reply: 3)

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