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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
31. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
32. Help with shRNAmiR30 based constructs (for a simple MD) - (reply: 3)
33. Question: How to differentiate siRNAs and mature miRNAs in plant by Northern blo - (reply: 3)
34. synthetic microRNA stability - (reply: 1)
35. siRNA detection......which strand shuld labelled - (reply: 2)
36. scrambeled miR sequence - (reply: 5)
37. miRNA overexpression troubleshooting - (reply: 2)
38. siRNA quantification using UV - (reply: 1)
39. overexpress mature microRNA by siRNA vector - (reply: 2)
40. MiRNA obtaining - (reply: 1)
41. Gene of interest targeting micrornas - (reply: 6)
42. Trouble with shRNA transfection - (reply: 2)
43. need help for trouble shooting the contamination in miR-X assay from serum - (reply: 1)
44. miRNA sequencing from FFPE tissue - (reply: 3)
45. which to use: miR or miRexpression vector - (reply: 1)
46. RNA journals - (reply: 4)
47. Detecting miRNA mimics by RT-PCR? - (reply: 4)
48. shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages - (reply: 1)
49. RNA alcohol/salt precipitation...low temperature - (reply: 1)
50. To small amount of cells used for shRNA transfection - (reply: 1)
51. shRNA interference using Lenti virus vector - (reply: 1)
52. Dual Luciferase Assay - (reply: 1)
53. Overexpressing miR - rescue by co-overexpressing its target - (reply: 1)
54. miRNA quantification - (reply: 3)
55. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
56. Gene knockdown expression levels shRNAmirs - (reply: 2)
57. siRNA in E.Coli - (reply: 2)
58. siRNA resuspension in RNase-free water - (reply: 1)
59. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
60. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)

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