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31. What is the best way to overexpress miRNAs? - (reply: 1)
32. Hiperfect siRNA in HT-29 cells help - (reply: 2)
33. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
34. Different amount of oligonucleotide - (reply: 1)
35. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
36. siRNA modifications - (reply: 2)
37. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
38. shRNA problem - Higher expression at the mRNA level ! - (reply: 4)
39. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
40. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)
41. validation of microRNA target gene - (reply: 6)
42. Can I use custom made synthetic miRNA for Overexpression? - (reply: 1)
43. Little knockdown but big effect on proliferation - (reply: 2)
44. Knockdown induce apoptosis - (reply: 5)
45. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
46. Converting shRNA sequence to siRNA - (reply: 1)
47. Can not reduce protein level - (reply: 1)
48. target mRNA/protein levels increase with siRNA - (reply: 2)
49. One siRNA sequence, many cell lines - (reply: 1)
50. miRNA from plasma_purity problem after RNA isolation - (reply: 1)
51. Substituting miRNA with ODNs for testing efficiency of dendrimers...? - (reply: 4)
52. RNAi in combination with adenoviral-mediated over-expression - (reply: 1)
53. microRNA exiqon individual assays - (reply: 1)
54. Oligo primers for shRNA construction - (reply: 1)
55. Antagomir design tool - (reply: 3)
56. Guide vs passenger strand sorting - (reply: 1)
57. How to explain this result? - (reply: 1)
58. Need an effective siRNA/ shRNA sequence for downregulating caveolin-1 - (reply: 2)
59. Higher mRNA level after RNAi? - (reply: 1)
60. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
32. Hiperfect siRNA in HT-29 cells help - (reply: 2)
33. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
34. Different amount of oligonucleotide - (reply: 1)
35. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
36. siRNA modifications - (reply: 2)
37. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
38. shRNA problem - Higher expression at the mRNA level ! - (reply: 4)
39. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
40. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)
41. validation of microRNA target gene - (reply: 6)
42. Can I use custom made synthetic miRNA for Overexpression? - (reply: 1)
43. Little knockdown but big effect on proliferation - (reply: 2)
44. Knockdown induce apoptosis - (reply: 5)
45. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
46. Converting shRNA sequence to siRNA - (reply: 1)
47. Can not reduce protein level - (reply: 1)
48. target mRNA/protein levels increase with siRNA - (reply: 2)
49. One siRNA sequence, many cell lines - (reply: 1)
50. miRNA from plasma_purity problem after RNA isolation - (reply: 1)
51. Substituting miRNA with ODNs for testing efficiency of dendrimers...? - (reply: 4)
52. RNAi in combination with adenoviral-mediated over-expression - (reply: 1)
53. microRNA exiqon individual assays - (reply: 1)
54. Oligo primers for shRNA construction - (reply: 1)
55. Antagomir design tool - (reply: 3)
56. Guide vs passenger strand sorting - (reply: 1)
57. How to explain this result? - (reply: 1)
58. Need an effective siRNA/ shRNA sequence for downregulating caveolin-1 - (reply: 2)
59. Higher mRNA level after RNAi? - (reply: 1)
60. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)