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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
31. MiRNA obtaining - (reply: 1)
32. Gene of interest targeting micrornas - (reply: 6)
33. Trouble with shRNA transfection - (reply: 2)
34. need help for trouble shooting the contamination in miR-X assay from serum - (reply: 1)
35. miRNA sequencing from FFPE tissue - (reply: 3)
36. which to use: miR or miRexpression vector - (reply: 1)
37. RNA journals - (reply: 4)
38. Detecting miRNA mimics by RT-PCR? - (reply: 4)
39. shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages - (reply: 1)
40. RNA alcohol/salt precipitation...low temperature - (reply: 1)
41. To small amount of cells used for shRNA transfection - (reply: 1)
42. shRNA interference using Lenti virus vector - (reply: 1)
43. Dual Luciferase Assay - (reply: 1)
44. Overexpressing miR - rescue by co-overexpressing its target - (reply: 1)
45. miRNA quantification - (reply: 3)
46. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
47. Gene knockdown expression levels shRNAmirs - (reply: 2)
48. siRNA in E.Coli - (reply: 2)
49. siRNA resuspension in RNase-free water - (reply: 1)
50. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
51. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
52. microRNA promoter based expression vector? - (reply: 1)
53. miRNA expression profiling - (reply: 3)
54. microRNA validation in vivo - (reply: 1)
55. in vivo naked-mRNA delivery - (reply: 4)
56. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
57. Recommendation on siRNA transfection reagents - (reply: 2)
58. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
59. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
60. Transfection reagent stored at -20C - (reply: 2)

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