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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
31. overexpress mature microRNA by siRNA vector - (reply: 2)
32. MiRNA obtaining - (reply: 1)
33. Gene of interest targeting micrornas - (reply: 6)
34. Trouble with shRNA transfection - (reply: 2)
35. need help for trouble shooting the contamination in miR-X assay from serum - (reply: 1)
36. miRNA sequencing from FFPE tissue - (reply: 3)
37. which to use: miR or miRexpression vector - (reply: 1)
38. RNA journals - (reply: 4)
39. Detecting miRNA mimics by RT-PCR? - (reply: 4)
40. shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages - (reply: 1)
41. RNA alcohol/salt precipitation...low temperature - (reply: 1)
42. To small amount of cells used for shRNA transfection - (reply: 1)
43. shRNA interference using Lenti virus vector - (reply: 1)
44. Dual Luciferase Assay - (reply: 1)
45. Overexpressing miR - rescue by co-overexpressing its target - (reply: 1)
46. miRNA quantification - (reply: 3)
47. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
48. Gene knockdown expression levels shRNAmirs - (reply: 2)
49. siRNA in E.Coli - (reply: 2)
50. siRNA resuspension in RNase-free water - (reply: 1)
51. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
52. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
53. microRNA promoter based expression vector? - (reply: 1)
54. miRNA expression profiling - (reply: 3)
55. microRNA validation in vivo - (reply: 1)
56. in vivo naked-mRNA delivery - (reply: 4)
57. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
58. Recommendation on siRNA transfection reagents - (reply: 2)
59. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
60. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)

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