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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
31. miRNA overexpression troubleshooting - (reply: 2)
32. siRNA quantification using UV - (reply: 1)
33. overexpress mature microRNA by siRNA vector - (reply: 2)
34. MiRNA obtaining - (reply: 1)
35. Gene of interest targeting micrornas - (reply: 6)
36. Trouble with shRNA transfection - (reply: 2)
37. need help for trouble shooting the contamination in miR-X assay from serum - (reply: 1)
38. miRNA sequencing from FFPE tissue - (reply: 3)
39. which to use: miR or miRexpression vector - (reply: 1)
40. RNA journals - (reply: 4)
41. Detecting miRNA mimics by RT-PCR? - (reply: 4)
42. shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages - (reply: 1)
43. RNA alcohol/salt precipitation...low temperature - (reply: 1)
44. To small amount of cells used for shRNA transfection - (reply: 1)
45. shRNA interference using Lenti virus vector - (reply: 1)
46. Dual Luciferase Assay - (reply: 1)
47. Overexpressing miR - rescue by co-overexpressing its target - (reply: 1)
48. miRNA quantification - (reply: 3)
49. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
50. Gene knockdown expression levels shRNAmirs - (reply: 2)
51. siRNA in E.Coli - (reply: 2)
52. siRNA resuspension in RNase-free water - (reply: 1)
53. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
54. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
55. microRNA promoter based expression vector? - (reply: 1)
56. miRNA expression profiling - (reply: 3)
57. microRNA validation in vivo - (reply: 1)
58. in vivo naked-mRNA delivery - (reply: 4)
59. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
60. Recommendation on siRNA transfection reagents - (reply: 2)

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