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181. Obtaining 3'UTR and perform microRNA target prediction - How to commence? (reply: 2)
182. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
183. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
184. how long will it takes to silence target gene after induction of shRNA - (reply: 2)
185. Protein shows KD but RNA level is higher - (reply: 4)
186. miRNA spike-in control - (reply: 6)
187. shRNAs work in vitro, not in vivo - (reply: 10)
188. Importin alpha siRNA - Looking for siRNA for knockdown of all Importin alpha subtypes (reply: 2)
189. miRNA 3ŽUTR target expression vectors - (reply: 6)
190. shRNA - (reply: 4)
191. nucleic acid extraction from silenced cells - (reply: 6)
192. Is there any pre-miRNA located in exon? - (reply: 1)
193. Differences between lentiviral packaging kits - (reply: 1)
194. siRNA overhangs - (reply: 2)
195. Can miRNAs regulate miRNA expression - (reply: 3)
196. Problem in shRNA Annelaing ( Help Required ) - (reply: 2)
197. siRNA in the Fridge - (reply: 2)
198. Lentivirus production with LNCX vector - Do I have to use Packaging cell lines? (reply: 3)
199. miRNA directed upregulation?! - (reply: 3)
200. microRNA profiling using ABI taqman low density microRNA array (TLDA) - (reply: 1)
201. Transfection - (reply: 1)
202. Double transfection of siRNA - (reply: 2)
203. AntimiR caused increased expression of miRNA - ANTIMIR (ambion) (reply: 6)
204. how to expression mirna in a specific tissue ？ - (reply: 1)
205. Problems ligating shRNA primers - Sequencing reveals damaged DNA (reply: 1)
206. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
207. the necessity of microRNA mutation design - (reply: 1)
208. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
209. transfection efficiency - (reply: 1)
210. Designing siRNA from Sequence provided by Qiagen - How to design from a given Nucleotide sequence a siRNA?? (reply: 4)
182. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
183. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
184. how long will it takes to silence target gene after induction of shRNA - (reply: 2)
185. Protein shows KD but RNA level is higher - (reply: 4)
186. miRNA spike-in control - (reply: 6)
187. shRNAs work in vitro, not in vivo - (reply: 10)
188. Importin alpha siRNA - Looking for siRNA for knockdown of all Importin alpha subtypes (reply: 2)
189. miRNA 3ŽUTR target expression vectors - (reply: 6)
190. shRNA - (reply: 4)
191. nucleic acid extraction from silenced cells - (reply: 6)
192. Is there any pre-miRNA located in exon? - (reply: 1)
193. Differences between lentiviral packaging kits - (reply: 1)
194. siRNA overhangs - (reply: 2)
195. Can miRNAs regulate miRNA expression - (reply: 3)
196. Problem in shRNA Annelaing ( Help Required ) - (reply: 2)
197. siRNA in the Fridge - (reply: 2)
198. Lentivirus production with LNCX vector - Do I have to use Packaging cell lines? (reply: 3)
199. miRNA directed upregulation?! - (reply: 3)
200. microRNA profiling using ABI taqman low density microRNA array (TLDA) - (reply: 1)
201. Transfection - (reply: 1)
202. Double transfection of siRNA - (reply: 2)
203. AntimiR caused increased expression of miRNA - ANTIMIR (ambion) (reply: 6)
204. how to expression mirna in a specific tissue ？ - (reply: 1)
205. Problems ligating shRNA primers - Sequencing reveals damaged DNA (reply: 1)
206. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
207. the necessity of microRNA mutation design - (reply: 1)
208. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
209. transfection efficiency - (reply: 1)
210. Designing siRNA from Sequence provided by Qiagen - How to design from a given Nucleotide sequence a siRNA?? (reply: 4)