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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
61. To small amount of cells used for shRNA transfection - (reply: 1)
62. shRNA interference using Lenti virus vector - (reply: 1)
63. Dual Luciferase Assay - (reply: 1)
64. Overexpressing miR - rescue by co-overexpressing its target - (reply: 1)
65. miRNA quantification - (reply: 3)
66. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
67. Gene knockdown expression levels shRNAmirs - (reply: 2)
68. siRNA in E.Coli - (reply: 2)
69. siRNA resuspension in RNase-free water - (reply: 1)
70. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
71. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
72. microRNA promoter based expression vector? - (reply: 1)
73. miRNA expression profiling - (reply: 3)
74. microRNA validation in vivo - (reply: 1)
75. in vivo naked-mRNA delivery - (reply: 4)
76. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
77. Recommendation on siRNA transfection reagents - (reply: 2)
78. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
79. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
80. Transfection reagent stored at -20C - (reply: 2)
81. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
82. Synthetic microrna for normalization in serum samples - (reply: 1)
83. DNA and siRNA transfection and cytotoxicity - (reply: 1)
84. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
85. What is the best way to overexpress miRNAs? - (reply: 1)
86. Hiperfect siRNA in HT-29 cells help - (reply: 2)
87. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
88. Different amount of oligonucleotide - (reply: 1)
89. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
90. siRNA modifications - (reply: 2)

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