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61. si/shRNA sequences against eGFP - (reply: 1)
62. microRNA and 3' UTR - (reply: 2)
63. Designing primers for miRNA located on minus strand. - (reply: 2)
64. Need help with extracting miRNA from human serum using mirVana kit - (reply: 2)
65. siRNA time - (reply: 1)
66. mir30-based shrna cloning-mismatch - (reply: 1)
67. New RNAi video from Nature - (reply: 1)
68. checking whther there is any miRNA in introns of a specific gene. - (reply: 1)
69. What to do Post-transfection of cells with siRNA? - (reply: 2)
70. miRNA in serum - (reply: 1)
71. Difference between <Antisense RNA> & <RNAi>? - (reply: 1)
72. How to predict targets from a novel miRNA - (reply: 1)
73. Knock down efficiency - (reply: 2)
74. Let-7 and miR-143 miRNA? - (reply: 1)
75. protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
76. Targets of mirna* - (reply: 1)
77. Which company to buy siRNA from? - (reply: 1)
78. How to predict targets of unkown miRNAs in Drosophila - (reply: 2)
79. shRNA rescue with site directed mutagenesis - oligo design - (reply: 4)
80. How to explain the difference between microarray results and qPCR data - (reply: 1)
81. placing siRNA seq into miRNA stem-loop - (reply: 4)
82. How many RNA I need for detecting plasma miR expression? - (reply: 1)
83. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
84. Totai RNA dilution - (reply: 8)
85. Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
86. problem with miRNA functional study - (reply: 1)
87. problem with siRNA transfection of HEK cells - (reply: 5)
88. Decreased siRNA performance at high concentrations: feasible? - (reply: 1)
89. Basic question: sc- vs. sc+ - (reply: 2)
90. 3'UTR sequence of a gene - (reply: 3)
62. microRNA and 3' UTR - (reply: 2)
63. Designing primers for miRNA located on minus strand. - (reply: 2)
64. Need help with extracting miRNA from human serum using mirVana kit - (reply: 2)
65. siRNA time - (reply: 1)
66. mir30-based shrna cloning-mismatch - (reply: 1)
67. New RNAi video from Nature - (reply: 1)
68. checking whther there is any miRNA in introns of a specific gene. - (reply: 1)
69. What to do Post-transfection of cells with siRNA? - (reply: 2)
70. miRNA in serum - (reply: 1)
71. Difference between <Antisense RNA> & <RNAi>? - (reply: 1)
72. How to predict targets from a novel miRNA - (reply: 1)
73. Knock down efficiency - (reply: 2)
74. Let-7 and miR-143 miRNA? - (reply: 1)
75. protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
76. Targets of mirna* - (reply: 1)
77. Which company to buy siRNA from? - (reply: 1)
78. How to predict targets of unkown miRNAs in Drosophila - (reply: 2)
79. shRNA rescue with site directed mutagenesis - oligo design - (reply: 4)
80. How to explain the difference between microarray results and qPCR data - (reply: 1)
81. placing siRNA seq into miRNA stem-loop - (reply: 4)
82. How many RNA I need for detecting plasma miR expression? - (reply: 1)
83. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
84. Totai RNA dilution - (reply: 8)
85. Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
86. problem with miRNA functional study - (reply: 1)
87. problem with siRNA transfection of HEK cells - (reply: 5)
88. Decreased siRNA performance at high concentrations: feasible? - (reply: 1)
89. Basic question: sc- vs. sc+ - (reply: 2)
90. 3'UTR sequence of a gene - (reply: 3)