Protocol Online logo
Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
61. RNA alcohol/salt precipitation...low temperature - (reply: 1)
62. To small amount of cells used for shRNA transfection - (reply: 1)
63. shRNA interference using Lenti virus vector - (reply: 1)
64. Dual Luciferase Assay - (reply: 1)
65. Overexpressing miR - rescue by co-overexpressing its target - (reply: 1)
66. miRNA quantification - (reply: 3)
67. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
68. Gene knockdown expression levels shRNAmirs - (reply: 2)
69. siRNA in E.Coli - (reply: 2)
70. siRNA resuspension in RNase-free water - (reply: 1)
71. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
72. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
73. microRNA promoter based expression vector? - (reply: 1)
74. miRNA expression profiling - (reply: 3)
75. microRNA validation in vivo - (reply: 1)
76. in vivo naked-mRNA delivery - (reply: 4)
77. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
78. Recommendation on siRNA transfection reagents - (reply: 2)
79. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
80. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
81. Transfection reagent stored at -20C - (reply: 2)
82. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
83. Synthetic microrna for normalization in serum samples - (reply: 1)
84. DNA and siRNA transfection and cytotoxicity - (reply: 1)
85. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
86. What is the best way to overexpress miRNAs? - (reply: 1)
87. Hiperfect siRNA in HT-29 cells help - (reply: 2)
88. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
89. Different amount of oligonucleotide - (reply: 1)
90. Looking for tool that searches a list of genes for common MiRs - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15