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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
61. miRNA quantification - (reply: 3)
62. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
63. Gene knockdown expression levels shRNAmirs - (reply: 2)
64. siRNA in E.Coli - (reply: 2)
65. siRNA resuspension in RNase-free water - (reply: 1)
66. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
67. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
68. microRNA promoter based expression vector? - (reply: 1)
69. miRNA expression profiling - (reply: 3)
70. microRNA validation in vivo - (reply: 1)
71. in vivo naked-mRNA delivery - (reply: 4)
72. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
73. Recommendation on siRNA transfection reagents - (reply: 2)
74. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
75. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
76. Transfection reagent stored at -20C - (reply: 2)
77. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
78. Synthetic microrna for normalization in serum samples - (reply: 1)
79. DNA and siRNA transfection and cytotoxicity - (reply: 1)
80. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
81. What is the best way to overexpress miRNAs? - (reply: 1)
82. Hiperfect siRNA in HT-29 cells help - (reply: 2)
83. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
84. Different amount of oligonucleotide - (reply: 1)
85. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
86. siRNA modifications - (reply: 2)
87. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
88. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)
89. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
90. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)

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