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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
61. siRNA resuspension in RNase-free water - (reply: 1)
62. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
63. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
64. microRNA promoter based expression vector? - (reply: 1)
65. miRNA expression profiling - (reply: 3)
66. microRNA validation in vivo - (reply: 1)
67. in vivo naked-mRNA delivery - (reply: 4)
68. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
69. Recommendation on siRNA transfection reagents - (reply: 2)
70. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
71. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
72. Transfection reagent stored at -20C - (reply: 2)
73. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
74. Synthetic microrna for normalization in serum samples - (reply: 1)
75. DNA and siRNA transfection and cytotoxicity - (reply: 1)
76. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
77. What is the best way to overexpress miRNAs? - (reply: 1)
78. Hiperfect siRNA in HT-29 cells help - (reply: 2)
79. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
80. Different amount of oligonucleotide - (reply: 1)
81. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
82. siRNA modifications - (reply: 2)
83. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
84. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)
85. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
86. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)
87. validation of microRNA target gene - (reply: 6)
88. Can I use custom made synthetic miRNA for Overexpression? - (reply: 1)
89. Little knockdown but big effect on proliferation - (reply: 2)
90. Knockdown induce apoptosis - (reply: 5)

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