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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
61. in vivo naked-mRNA delivery - (reply: 4)
62. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
63. Recommendation on siRNA transfection reagents - (reply: 2)
64. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
65. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
66. Transfection reagent stored at -20C - (reply: 2)
67. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
68. Synthetic microrna for normalization in serum samples - (reply: 1)
69. DNA and siRNA transfection and cytotoxicity - (reply: 1)
70. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
71. What is the best way to overexpress miRNAs? - (reply: 1)
72. Hiperfect siRNA in HT-29 cells help - (reply: 2)
73. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
74. Different amount of oligonucleotide - (reply: 1)
75. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
76. siRNA modifications - (reply: 2)
77. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
78. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)
79. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
80. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)
81. validation of microRNA target gene - (reply: 6)
82. Can I use custom made synthetic miRNA for Overexpression? - (reply: 1)
83. Little knockdown but big effect on proliferation - (reply: 2)
84. Knockdown induce apoptosis - (reply: 5)
85. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
86. Converting shRNA sequence to siRNA - (reply: 1)
87. Can not reduce protein level - (reply: 1)
88. target mRNA/protein levels increase with siRNA - (reply: 2)
89. One siRNA sequence, many cell lines - (reply: 1)
90. miRNA from plasma_purity problem after RNA isolation - (reply: 1)

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