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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
61. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
62. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
63. microRNA promoter based expression vector? - (reply: 1)
64. miRNA expression profiling - (reply: 3)
65. microRNA validation in vivo - (reply: 1)
66. in vivo naked-mRNA delivery - (reply: 4)
67. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
68. Recommendation on siRNA transfection reagents - (reply: 2)
69. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
70. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
71. Transfection reagent stored at -20C - (reply: 2)
72. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
73. Synthetic microrna for normalization in serum samples - (reply: 1)
74. DNA and siRNA transfection and cytotoxicity - (reply: 1)
75. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
76. What is the best way to overexpress miRNAs? - (reply: 1)
77. Hiperfect siRNA in HT-29 cells help - (reply: 2)
78. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
79. Different amount of oligonucleotide - (reply: 1)
80. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
81. siRNA modifications - (reply: 2)
82. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
83. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)
84. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
85. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)
86. validation of microRNA target gene - (reply: 6)
87. Can I use custom made synthetic miRNA for Overexpression? - (reply: 1)
88. Little knockdown but big effect on proliferation - (reply: 2)
89. Knockdown induce apoptosis - (reply: 5)
90. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)

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