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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
61. Dual Luciferase Assay - (reply: 1)
62. Overexpressing miR - rescue by co-overexpressing its target - (reply: 1)
63. miRNA quantification - (reply: 3)
64. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
65. Gene knockdown expression levels shRNAmirs - (reply: 2)
66. siRNA in E.Coli - (reply: 2)
67. siRNA resuspension in RNase-free water - (reply: 1)
68. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
69. Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
70. microRNA promoter based expression vector? - (reply: 1)
71. miRNA expression profiling - (reply: 3)
72. microRNA validation in vivo - (reply: 1)
73. in vivo naked-mRNA delivery - (reply: 4)
74. shRNA with 100% marker expression but no knockdown efficiency at all - (reply: 7)
75. Recommendation on siRNA transfection reagents - (reply: 2)
76. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
77. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
78. Transfection reagent stored at -20C - (reply: 2)
79. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
80. Synthetic microrna for normalization in serum samples - (reply: 1)
81. DNA and siRNA transfection and cytotoxicity - (reply: 1)
82. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
83. What is the best way to overexpress miRNAs? - (reply: 1)
84. Hiperfect siRNA in HT-29 cells help - (reply: 2)
85. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
86. Different amount of oligonucleotide - (reply: 1)
87. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
88. siRNA modifications - (reply: 2)
89. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
90. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)

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