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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
61. DNA and siRNA transfection and cytotoxicity - (reply: 1)
62. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
63. What is the best way to overexpress miRNAs? - (reply: 1)
64. Hiperfect siRNA in HT-29 cells help - (reply: 2)
65. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
66. Different amount of oligonucleotide - (reply: 1)
67. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
68. siRNA modifications - (reply: 2)
69. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
70. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)
71. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
72. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)
73. validation of microRNA target gene - (reply: 6)
74. Can I use custom made synthetic miRNA for Overexpression? - (reply: 1)
75. Little knockdown but big effect on proliferation - (reply: 2)
76. Knockdown induce apoptosis - (reply: 5)
77. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
78. Converting shRNA sequence to siRNA - (reply: 1)
79. Can not reduce protein level - (reply: 1)
80. target mRNA/protein levels increase with siRNA - (reply: 2)
81. One siRNA sequence, many cell lines - (reply: 1)
82. miRNA from plasma_purity problem after RNA isolation - (reply: 1)
83. Substituting miRNA with ODNs for testing efficiency of dendrimers...? - (reply: 4)
84. RNAi in combination with adenoviral-mediated over-expression - (reply: 1)
85. microRNA exiqon individual assays - (reply: 1)
86. Oligo primers for shRNA construction - (reply: 1)
87. Antagomir design tool - (reply: 3)
88. Guide vs passenger strand sorting - (reply: 1)
89. How to explain this result? - (reply: 1)
90. Need an effective siRNA/ shRNA sequence for downregulating caveolin-1 - (reply: 2)

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