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Top : New Forum Archives (2009-): : siRNA--microRNA-and-RNAi
61. Transfection reagent stored at -20C - (reply: 2)
62. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
63. Synthetic microrna for normalization in serum samples - (reply: 1)
64. DNA and siRNA transfection and cytotoxicity - (reply: 1)
65. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
66. What is the best way to overexpress miRNAs? - (reply: 1)
67. Hiperfect siRNA in HT-29 cells help - (reply: 2)
68. Is the distance between U6 promoter and shRNA sequence critical? - (reply: 6)
69. Different amount of oligonucleotide - (reply: 1)
70. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
71. siRNA modifications - (reply: 2)
72. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
73. shRNA problem - Higher expression at the mRNA level ! - (reply: 5)
74. 3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
75. Cannot get good knockdown with multiple shRNA constructs - (reply: 4)
76. validation of microRNA target gene - (reply: 6)
77. Can I use custom made synthetic miRNA for Overexpression? - (reply: 1)
78. Little knockdown but big effect on proliferation - (reply: 2)
79. Knockdown induce apoptosis - (reply: 5)
80. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
81. Converting shRNA sequence to siRNA - (reply: 1)
82. Can not reduce protein level - (reply: 1)
83. target mRNA/protein levels increase with siRNA - (reply: 2)
84. One siRNA sequence, many cell lines - (reply: 1)
85. miRNA from plasma_purity problem after RNA isolation - (reply: 1)
86. Substituting miRNA with ODNs for testing efficiency of dendrimers...? - (reply: 4)
87. RNAi in combination with adenoviral-mediated over-expression - (reply: 1)
88. microRNA exiqon individual assays - (reply: 1)
89. Oligo primers for shRNA construction - (reply: 1)
90. Antagomir design tool - (reply: 3)

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