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Top : New Forum Archives (2009-): : Tissue-and-Cell-Culture
1021. U-937 scale up - (reply: 11)
1022. Fixing tissue - (reply: 7)
1023. Transwell plates - How to work properly (reply: 4)
1024. Cell migration assay with 3 cell types - (reply: 1)
1025. Conditioned media preparation - (reply: 3)
1026. How to infect 7.5kb insert? - (reply: 7)
1027. Do histology staningings work in cell culture? - (reply: 2)
1028. Stimulating/Drugging Mammilian Cells - What is the proper way to add drugs or stimulants to a cell culture? (reply: 3)
1029. Primary cerebellar neurons and proteasome assay - (reply: 2)
1030. G418 and C6 Glioma Death / Stable C6 Line - (reply: 1)
1031. Co-transfection of two plasmids - Need the protocol for lipofectamine 2000 based transfection (reply: 5)
1032. Subculture in low glucose media - (reply: 2)
1033. type of rat hepatic cell for toxicology study - seeking opinion on what type of cell to be used (reply: 1)
1034. CHO Cell contamination - (reply: 6)
1035. solve MCF-7 culture trouble - (reply: 4)
1036. Prevention of fungal contamination - (reply: 4)
1037. Primary muscle cultures - Keeping myoblasts alive! (reply: 1)
1038. HL-60 cultures - (reply: 1)
1039. DMSO and cellular culture - (reply: 6)
1040. DMEM vs RPMI for primary mouse lymphocytes - Which media for primary mouse lymphocyte? (reply: 2)
1041. How to coat matrigel more smoothly? - (reply: 2)
1042. MTT OD values: 490nm? 570nm? or 630nm? - (reply: 1)
1043. Cell culture contamination - "outsider I.D." (reply: 2)
1044. Volume of 24 well plate - (reply: 1)
1045. Alternative media for PC-12 cells - (reply: 2)
1046. viability of adherent cells - (reply: 3)
1047. Background Immunofluorescence - Suggestions on how to reduce non-specific background (reply: 3)
1048. Trypsin the cell killer - How much death does trypsin actually cause (reply: 2)
1049. How to keep from causing damage? - (reply: 1)
1050. How to grow cell in the iron-deficient environment? - 4T1 cell. (reply: 1)