Plateau or saturation density - (Jun/06/2010 )
Can anyone tell how to calculate the plateau or suspension density for suspension cells in a T25 flask?
I know it's number of cells divided per cubic centimeter. but what is the volume of a T25 flask?
Density is a measure of number (of cells/particles anything really) per unit (area/volume/time/etc) it is independent of the total volume of your culture. For suspension cells you could culture cells in a t25 in as little as 3 ml or as much as 100 ml, with equal densities, but the total cell number will change depending on the volume.
to convert density to total cell number multiply the density (cells per ml) by the volume.