Protocol Online logo
Top : New Forum Archives (2009-): : Tissue-and-Cell-Culture
271. Subculture issue of primary cell - (reply: 6)
272. Differentiating mouse from human cells with PCR - (reply: 5)
273. Problems with extraction of RNA from primary culture - (reply: 4)
274. Manual/ User guide for opticell from thermoscientific - (reply: 2)
275. What to do about mycoplasma? - (reply: 9)
276. collagen coating of transwell inserts - (reply: 2)
277. Mycoplasma Test Kit - (reply: 1)
278. How to transform adherent cells to suspension (CHO cells) - (reply: 2)
279. Can I make an SDS stock in media and store? - (reply: 3)
280. Cell attachement occuring after 48 hours, not 24 hours. - (reply: 3)
281. How to graph or plot MTT assay data - (reply: 10)
282. Quick question about making culture media. - (reply: 5)
283. New to organotypic slice cultures: my slices seem to disintegrate during stainin - (reply: 1)
284. Procedures to replace vial of cells and passage number. - (reply: 4)
285. Immunofluorescence on cells - can I keep the coverslips unstained in PFA? - (reply: 4)
286. Using Labtek for Bone marrow macrophages - (reply: 1)
287. Generation of single cell suspensions from neural tissue - (reply: 2)
288. Is this contamination or cell death? - (reply: 7)
289. THP-1 differentiated cells detach when washing with PBS (Mg and Ca free PBS) - (reply: 2)
290. unwanted cell lysis during LB+Amp incubation - (reply: 5)
291. Cells spontaneously detaching? - (reply: 1)
292. Maintainence of confluent cultures - (reply: 1)
293. different type of media for cell culture - (reply: 1)
294. How to treat cells with TNFa - (reply: 2)
295. CLOUDINESS of RIN5F cell culture - (reply: 7)
296. Which hydrogen peroxide? - (reply: 1)
297. U87MG cells not growing well - (reply: 3)
298. Concentrating low volume of cells - (reply: 8)
299. How does fibronectin "crash out" of solution? - (reply: 1)
300. Preparing a conditioned medium from cancer cells - (reply: 3)