Protocol Online logo
Top : New Forum Archives (2009-): : Tissue-and-Cell-Culture
601. Quenstion-Do you add the sodium pyruvate into the DMEM or 1640 meduim? - (reply: 6)
602. Different cell lines different protein stability - (reply: 1)
603. protein extraction from transwell inserts - (reply: 2)
604. TZM-bl Assay Problem - (reply: 2)
605. THP1 monocytes cultured forms "vacuols-like" compartments - (reply: 1)
606. Question about suspension cells - (reply: 7)
607. Calu-3 cell line do not grow properly - (reply: 1)
608. Counting and plating cells - (reply: 3)
609. cells die 4-6 days after thawing - (reply: 8)
610. what is the trypsin X for subculturing - (reply: 1)
611. Fugene6 Calculations aren't working....please advise??? - (reply: 6)
612. Oil red O staining?? - (reply: 1)
613. long term storage of cells at -80 - (reply: 1)
614. How long do growth factors last in complete media? - (reply: 3)
615. Antibiotic concentration in primary culture - (reply: 4)
616. Hacat Seeding into 8-well chamber slide - (reply: 1)
617. cell plating density - (reply: 2)
618. What kind of cell culture contamination is it? - (reply: 8)
619. Problem with preparing unicellular suspension from MCF7 cells - (reply: 3)
620. Seeking advice Regarding contamination - (reply: 2)
621. xenograft cells harvested from spleen of SCID/NOD mice - (reply: 1)
622. 3T3-L1 differenciation and maintenance - (reply: 4)
623. Culturing cells on a slide - (reply: 5)
624. Can you freeze-thaw DMEM and it still work? - (reply: 7)
625. How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
626. What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
627. Could the use of different FBS affact my cells - (reply: 5)
628. Does DNA have to be supercoiled for transient protein expression? - (reply: 4)
629. Differences between J774.1 and RAW 264.7 murine macrophages? - (reply: 3)
630. High glucose for primary astrocyte or neurons? - (reply: 1)