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Top : New Forum Archives (2009-): : Tissue-and-Cell-Culture
181. Setting up two drug kill curve for selection of stable cells - (reply: 1)
182. using DMSO and Ethanol at the same time - (reply: 3)
183. No expression in stable transfected cell line - (reply: 2)
184. Cell line contamination? - (reply: 5)
185. Opti-MEM I Reduced Serum Medium - (reply: 1)
186. Puromycin selection versus FACS - (reply: 1)
187. Inducing apoptosis by puromycin selection: toxic to the selected cells? - (reply: 1)
188. Measuring cell proliferation from daily aliquots - (reply: 1)
189. Etoposide Drug assay - (reply: 1)
190. Help please, what is this unknown seen under my microscope - (reply: 2)
191. Cell culture of MYC immortalised HDFa - need advice - (reply: 1)
192. Harvesting cells - (reply: 12)
193. Tiny black dots inside cell vacuoles - (reply: 1)
194. I always have a cell depris floating in the supernatent of MDCK cells - (reply: 3)
195. can i use 50ml tube contain isopropanol alchol - (reply: 4)
196. Why do we use glycerol for bacterial stocks (frozen) and DMSO for eukaryotic cel - (reply: 1)
197. Treating suspension cells - (reply: 2)
198. How can i seed 2000 cell /well? (Using 200 microliter media and 96 plate, and as - (reply: 1)
199. Establishing human neural stem cells - (reply: 3)
200. Long term storage at -80C - (reply: 2)
201. FCS in Serum free medium for better Cell Adhesion? - (reply: 1)
202. Transient transfection with large plasmid - (reply: 3)
203. transfection using lipofectamine 2000 - (reply: 3)
204. can buffer ever go bad? - (reply: 2)
205. 293s vs HEK/293 cells - (reply: 2)
206. PBMC and granulocyte isolation from human whole blood - (reply: 1)
207. co-transfection - (reply: 2)
208. Cell culture medium contamination? - (reply: 1)
209. What do sick 293t cells look like? - (reply: 3)
210. How to design a time course experiment - (reply: 2)